PMID- 25413315
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141121
LR  - 20181113
IS  - 2051-817X (Print)
IS  - 2051-817X (Electronic)
IS  - 2051-817X (Linking)
VI  - 2
IP  - 11
DP  - 2014 Nov 1
TI  - MCP-1 downregulates MMP-9 export via vesicular redistribution to lysosomes in rat 
      portal fibroblasts.
LID - 10.14814/phy2.12153 [doi]
LID - e12153
AB  - Portal fibroblasts (PF) are one of the two primary cell types contributing to the 
      myofibroblast population of the liver and are thus essential to the pathogenesis 
      of liver fibrosis. Monocyte chemoattractant protein-1 (MCP-1) is a known 
      profibrogenic chemokine that may be of particular importance in biliary fibrosis. 
      We examined the effect of MCP-1 on release of matrix metalloproteinase-9 (MMP-9) 
      by rat PF. We found that MCP-1 blocks PF release of MMP-9 in a posttranslational 
      fashion. We employed an optical and electron microscopic approach to determine 
      the mechanism of this downregulation. Our data demonstrated that, in the presence 
      of MCP-1, MMP-9-containing vesicles were shunted to a lysosome-like compartment. 
      This is the first report of a secretory protein to be so regulated in fibrogenic 
      cells.
CI  - (c) 2014 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on 
      behalf of the American Physiological Society and The Physiological Society.
FAU - Hickman, DaShawn A
AU  - Hickman DA
AD  - Division of Gastroenterology and Hepatology, Department of Internal Medicine, 
      University of Arkansas for Medical Sciences, Little Rock, Arkansas.
FAU - Syal, Gaurav
AU  - Syal G
AD  - Division of Gastroenterology and Hepatology, Department of Internal Medicine, 
      University of Arkansas for Medical Sciences, Little Rock, Arkansas.
FAU - Fausther, Michel
AU  - Fausther M
AD  - Division of Gastroenterology and Hepatology, Department of Internal Medicine, 
      University of Arkansas for Medical Sciences, Little Rock, Arkansas.
FAU - Lavoie, Elise G
AU  - Lavoie EG
AD  - Division of Gastroenterology and Hepatology, Department of Internal Medicine, 
      University of Arkansas for Medical Sciences, Little Rock, Arkansas.
FAU - Goree, Jessica R
AU  - Goree JR
AD  - Division of Gastroenterology and Hepatology, Department of Internal Medicine, 
      University of Arkansas for Medical Sciences, Little Rock, Arkansas.
FAU - Storrie, Brian
AU  - Storrie B
AD  - Department of Physiology and Biophysics, University of Arkansas for Medical 
      Sciences, Little Rock, Arkansas.
FAU - Dranoff, Jonathan A
AU  - Dranoff JA
AD  - Division of Gastroenterology and Hepatology, Department of Internal Medicine, 
      University of Arkansas for Medical Sciences, Little Rock, Arkansas.
LA  - eng
PT  - Journal Article
DEP - 20141120
PL  - United States
TA  - Physiol Rep
JT  - Physiological reports
JID - 101607800
PMC - PMC4255798
OTO - NOTNLM
OT  - Lysosome
OT  - MCP-1
OT  - MMP-9
OT  - portal fibroblast
EDAT- 2014/11/22 06:00
MHDA- 2014/11/22 06:01
PMCR- 2014/11/20
CRDT- 2014/11/22 06:00
PHST- 2014/11/22 06:00 [entrez]
PHST- 2014/11/22 06:00 [pubmed]
PHST- 2014/11/22 06:01 [medline]
PHST- 2014/11/20 00:00 [pmc-release]
AID - 2/11/e12153 [pii]
AID - phy212153 [pii]
AID - 10.14814/phy2.12153 [doi]
PST - epublish
SO  - Physiol Rep. 2014 Nov 20;2(11):e12153. doi: 10.14814/phy2.12153. Print 2014 Nov 
      1.