PMID- 25413962
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20181202
IS  - 1559-0283 (Electronic)
IS  - 1085-9195 (Print)
IS  - 1085-9195 (Linking)
VI  - 71
IP  - 3
DP  - 2015 Apr
TI  - AKT Pathway Affects Bone Regeneration in Nonunion Treated with Umbilical 
      Cord-Derived Mesenchymal Stem Cells.
PG  - 1543-51
LID - 10.1007/s12013-014-0378-6 [doi]
AB  - We have previously grafted human umbilical cord-derived mesenchymal stem cells 
      (hUC-MSCs) with blood plasma to treat rat tibia nonunion. To further examine the 
      biological characteristics of this process, we applied an established 
      hUC-MSCs-treated rat nonunion model with the addition of an inhibitor of AKT. SD 
      rats (80) were randomly divided into four groups: a fracture group (positive 
      control); a nonunion group (negative control); a hUC-MSCs grafting with blood 
      plasma group; and a hUC-MSCs grafting with blood plasma & AKT blocker group. The 
      animals were sacrificed under deep anesthesia at 4 and 8 weeks post fracture for 
      analysis. The fracture line became less defined at 4 weeks and disappeared at 8 
      weeks postoperatively in both the hUC-MSCs grafting with blood plasma and 
      grafting with blood plasma & the AKT blocker, which is similar to the fracture 
      group. Histological immunofluorescence studies showed that the numbers of 
      hUC-MSCs in the calluses were significantly higher in the hUC-MSCs grafting with 
      blood plasma than those in group with the AKT blocker. More bone morphogenetic 
      protein 2 and bone sialoprotein expression and less osteoprotegerin and bone gla 
      protein expression were observed in the AKT blocker group compared to the 
      hUC-MSCs grafting with blood plasma. AKT gene expression in the AKT blocker group 
      was decreased 50% compared to the hUC-MSCs with plasma group and decreased 70% 
      compared to the fracture group, while the elastic modulus was decreased. In 
      summary, our work demonstrates that AKT may play a role in modulating 
      osteogenesis induced by hUC-MSCs.
FAU - Qu, Zhiguo
AU  - Qu Z
AD  - Department of Orthopaedic Surgery, Siping Hospital Affiliated to China Medical 
      University, Siping, Jilin, China.
AD  - Tuhua Bioengineering Company Ltd, Siping, Jilin, China.
FAU - Guo, Shengnan
AU  - Guo S
AD  - Tuhua Bioengineering Company Ltd, Siping, Jilin, China.
AD  - Department of Stem Cell Clinical Application Centre, Siping Hospital Affiliated 
      to China Medical University, No. 89, Nanyingbin Road, Tiexi District, Siping, 
      136000, Jilin, China.
FAU - Fang, Guojun
AU  - Fang G
AD  - Department of Orthopaedic Surgery, Siping Hospital Affiliated to China Medical 
      University, Siping, Jilin, China.
AD  - Tuhua Bioengineering Company Ltd, Siping, Jilin, China.
FAU - Cui, Zhenghong
AU  - Cui Z
AD  - Department of Orthopaedic Surgery, Siping Hospital Affiliated to China Medical 
      University, Siping, Jilin, China.
AD  - Tuhua Bioengineering Company Ltd, Siping, Jilin, China.
FAU - Liu, Ying
AU  - Liu Y
AD  - Department of Stem Cell Clinical Application Centre, Siping Hospital Affiliated 
      to China Medical University, No. 89, Nanyingbin Road, Tiexi District, Siping, 
      136000, Jilin, China. ly3641829@163.com.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cell Biochem Biophys
JT  - Cell biochemistry and biophysics
JID - 9701934
RN  - 0 (Enzyme Inhibitors)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Biomechanical Phenomena/drug effects
MH  - *Bone Regeneration/drug effects
MH  - Cell Adhesion
MH  - Cell Differentiation
MH  - Enzyme Inhibitors/pharmacology
MH  - Gene Expression Regulation, Enzymologic/drug effects
MH  - Humans
MH  - *Mesenchymal Stem Cell Transplantation
MH  - *Mesenchymal Stem Cells/cytology
MH  - Osteogenesis/drug effects
MH  - Proto-Oncogene Proteins c-akt/antagonists & inhibitors/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/drug effects
MH  - Tibial Fractures/pathology/physiopathology/surgery
MH  - Umbilical Cord/*cytology
PMC - PMC4449366
OTO - NOTNLM
OT  - AKT
OT  - BGP
OT  - BMP-2
OT  - BSP
OT  - Nonunion
OT  - OPG
OT  - hUC-MSC
EDAT- 2014/11/22 06:00
MHDA- 2016/12/15 06:00
PMCR- 2014/11/21
CRDT- 2014/11/22 06:00
PHST- 2014/11/22 06:00 [entrez]
PHST- 2014/11/22 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
PHST- 2014/11/21 00:00 [pmc-release]
AID - 10.1007/s12013-014-0378-6 [pii]
AID - 378 [pii]
AID - 10.1007/s12013-014-0378-6 [doi]
PST - ppublish
SO  - Cell Biochem Biophys. 2015 Apr;71(3):1543-51. doi: 10.1007/s12013-014-0378-6.