PMID- 25414029
OWN - NLM
STAT- MEDLINE
DCOM- 20150916
LR  - 20191113
IS  - 1875-5631 (Electronic)
IS  - 1566-5232 (Linking)
VI  - 15
IP  - 1
DP  - 2015
TI  - Transfection of CXCR-4 using microbubble-mediated ultrasound irradiation and 
      liposomes improves the migratory ability of bone marrow stromal cells.
PG  - 21-31
AB  - Bone marrow stromal cells (BMSCs) have proven useful for the treatment of various 
      human diseases and injuries. However, their reparative capacity is limited by 
      their poor migration and homing ability, which are primarily dependent on the 
      SDF-1/CXCR4 axis. Most subcultured BMSCs lack CXCR4 receptor expression on the 
      cell surface and exhibit impaired migratory capacity. To increase responsiveness 
      to SDF-1 and promote cell migration and survival of cultured BMSCs, we used a 
      combination of ultrasound-targeted microbubble destruction (UTMD) and liposomes 
      to increase CXCR4 expression in vitro. We isolated and cultured rat BMSCs to 
      their third passage and transduced them with recombinant plasmid pDsRed-CXCR4 
      using microbubble-mediated ultrasound irradiation and liposomes. Compared to some 
      viral vectors, the method we employed here resulted in significantly better 
      transfection efficiency, CXCR4 expression, and technical reproducibility. The 
      benefits of this approach are likely due to the combination of "sonoporation" 
      caused by shockwaves and microjet flow resulting from UTMD-generated cavitation. 
      Following transfection, we performed a transwell migration assay and found that 
      the migration ability of CXCR4-modified BMSCs was 9-fold higher than controls. 
      The methods we describe here provide an effective, safe, non-viral means to 
      achieve high levels of CXCR4 expression. This is associated with enhanced 
      migration of subcultured BMSCs and may be useful for clinical application as 
      well.
FAU - Wang, Gong
AU  - Wang G
FAU - Zhuo, Zhongxiong
AU  - Zhuo Z
FAU - Zhang, Qian
AU  - Zhang Q
FAU - Xu, Yali
AU  - Xu Y
FAU - Wu, Shengzheng
AU  - Wu S
FAU - Li, Lu
AU  - Li L
FAU - Xia, Hongmei
AU  - Xia H
FAU - Gao, Yunhua
AU  - Gao Y
AD  - Department of Ultrasound, Xinqiao Hospital, The Third Military Medical 
      University, Chongqing, 400037, China. xqgaoyh@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United Arab Emirates
TA  - Curr Gene Ther
JT  - Current gene therapy
JID - 101125446
RN  - 0 (Cxcr4 protein, rat)
RN  - 0 (Liposomes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CXCR4)
SB  - IM
MH  - Animals
MH  - *Cell Movement
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Gene Expression Regulation
MH  - Liposomes
MH  - Mesenchymal Stem Cells/*cytology
MH  - *Microbubbles
MH  - Microscopy, Electron, Scanning
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, CXCR4/*genetics/metabolism
MH  - Reproducibility of Results
MH  - Transfection/*methods
MH  - *Ultrasonics
EDAT- 2014/11/22 06:00
MHDA- 2015/09/17 06:00
CRDT- 2014/11/22 06:00
PHST- 2014/07/11 00:00 [received]
PHST- 2014/09/16 00:00 [revised]
PHST- 2014/10/31 00:00 [accepted]
PHST- 2014/11/22 06:00 [entrez]
PHST- 2014/11/22 06:00 [pubmed]
PHST- 2015/09/17 06:00 [medline]
AID - CGT-EPUB-63502 [pii]
AID - 10.2174/1566523214666141121111220 [doi]
PST - ppublish
SO  - Curr Gene Ther. 2015;15(1):21-31. doi: 10.2174/1566523214666141121111220.