PMID- 25414189
OWN - NLM
STAT- MEDLINE
DCOM- 20150302
LR  - 20220310
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 55
IP  - 12
DP  - 2014 Nov 20
TI  - Monitoring of strain-dependent responsiveness to TLR activation in the mouse 
      anterior segment using SD-OCT.
PG  - 8189-99
LID - 10.1167/iovs.14-15595 [doi]
AB  - PURPOSE: To determine whether spectral-domain optical coherence tomography 
      (SD-OCT) can be used to longitudinally monitor inflammation in the mouse anterior 
      segment and to identify any strain-dependent differences in responsiveness to 
      distinct toll-like receptor (TLR) ligands. METHODS: Corneal inflammation was 
      induced in BALB/c and C57BL/6 mice following central corneal abrasions and 
      topical application of saline, TLR-4 ligand, lipopolysaccharide (LPS), or TLR-9 
      ligand, CpG-oligodeoxynucleotide (CpG-ODN; CpG). Anterior-segment images were 
      captured using SD-OCT at baseline, 24 hours, and 1 week post treatment. Corneal 
      thickness, stromal haze, and the number of keratic precipitates (KP) and anterior 
      chamber (AC) cells were longitudinally compared to determine differences between 
      mouse strains, time points, and TLR activation. RESULTS: In both mouse strains, 
      treatment with CpG, but not saline or LPS, resulted in a similar number of KPs 
      and AC cells. In C57BL/6 mice, central corneal thickness (CCT) increased in CpG- 
      and LPS-treated eyes at 24 hours, which normalized by 1 week. In BALB/c mice, a 
      significant increase in CCT occurred in eyes treated with CpG at 1 week. Stromal 
      haze peaked in C57BL/6 eyes treated with LPS- or CPG-treatment at 24 hours; 
      however, BALB/c eyes showed persistent and marked increases in corneal haze 
      compared with baseline at 1 week post treatment. CONCLUSIONS: Spectral-domain OCT 
      enables high-resolution, longitudinal, in vivo imaging of anterior segment 
      inflammation in mice and revealed novel strain- and time-dependent differences in 
      response to distinct TLR activation in the cornea.
CI  - Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.
FAU - Downie, Laura Elizabeth
AU  - Downie LE
AD  - Department of Optometry and Vision Sciences, University of Melbourne, Parkville, 
      Victoria, Australia.
FAU - Stainer, Matthew James
AU  - Stainer MJ
AD  - Department of Optometry and Vision Sciences, University of Melbourne, Parkville, 
      Victoria, Australia.
FAU - Chinnery, Holly Rose
AU  - Chinnery HR
AD  - Department of Optometry and Vision Sciences, University of Melbourne, Parkville, 
      Victoria, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141120
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (CPG-oligonucleotide)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptor 9)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Anterior Chamber/cytology
MH  - Anterior Eye Segment/*drug effects/pathology
MH  - Corneal Edema/*diagnosis/drug therapy/pathology
MH  - Corneal Stroma/pathology
MH  - Disease Models, Animal
MH  - Female
MH  - Lipopolysaccharides/pharmacology
MH  - Longitudinal Studies
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Oligodeoxyribonucleotides/pharmacology
MH  - Toll-Like Receptor 4/*physiology
MH  - Toll-Like Receptor 9/*physiology
MH  - *Tomography, Optical Coherence
OTO - NOTNLM
OT  - SD-OCT
OT  - cornea
OT  - imaging
OT  - inflammation
OT  - toll-like receptors
EDAT- 2014/11/22 06:00
MHDA- 2015/03/03 06:00
CRDT- 2014/11/22 06:00
PHST- 2014/11/22 06:00 [entrez]
PHST- 2014/11/22 06:00 [pubmed]
PHST- 2015/03/03 06:00 [medline]
AID - iovs.14-15595 [pii]
AID - 10.1167/iovs.14-15595 [doi]
PST - epublish
SO  - Invest Ophthalmol Vis Sci. 2014 Nov 20;55(12):8189-99. doi: 
      10.1167/iovs.14-15595.