PMID- 25414784
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141121
LR  - 20220318
IS  - 2008-2835 (Print)
IS  - 2008-4625 (Electronic)
IS  - 2008-2835 (Linking)
VI  - 6
IP  - 4
DP  - 2014 Oct
TI  - Differentiation of human umbilical cord matrix-derived mesenchymal stem cells 
      into germ-like cells.
PG  - 218-27
AB  - BACKGROUND: Mesenchymal Stem Cells (MSCs) are multipotent cells that can be 
      collected from different sources. Under specific conditions, MSCs can be 
      differentiated to tissue specific cells in vitro. Human Umbilical Cord 
      mesenchymal Stem Cells (hUCMSCs) can easily be harvested and cultured in in vitro 
      conditions. Production of germ cells from mesenchymal stem cells is a very 
      interesting and promising area in the field of reproductive medicine. In the 
      present study, the possible trans-differentiation of hUCMSCs into Primordial like 
      Germ Cell (PGC) was performed in vitro under specific condition. METHODS: Human 
      umbilical cord mesenchymal stem cells were cultured and expanded in DMEM medium 
      containing 10% FBS. The cultured cells were studied for differentiation ability 
      to adipocytes and osteocytes. Furthermore, MSCs related markers were identified 
      by flow cytometry method. For PGC differentiation, hUCMS cells were cultured in 
      differentiation medium containing Bone Morphogenetic Protein 4 (BMP4) and it was 
      followed by retinoic acid (RA). Real time PCR and immunocytochemistry analysis 
      were performed to evaluate the expression of PGC specific genes and proteins, 
      respectively. RESULTS: Our results showed that hUCMSCs cultured in the presence 
      of BMP4 and RA are able to transdifferentiate in to PGC like cells in vitro. Real 
      time PCR and immunocytochemistry results showed that differentiated cells 
      expressed PGC specific markers after 14 days of culture. CONCLUSION: Based on 
      these results, it was concluded that hUCMSC may be considered as a promising 
      alternative cell source in reproductive medicine. More studies including 
      laboratory and also animal models are needed to evaluate the functionality of 
      differentiated PGCs before introducing them to clinical applications.
FAU - Latifpour, Mostafa
AU  - Latifpour M
AD  - Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, 
      Tehran, Iran.
FAU - Shakiba, Yadollah
AU  - Shakiba Y
AD  - Department of Immunology, School of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
FAU - Amidi, Fardin
AU  - Amidi F
AD  - Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, 
      Tehran, Iran.
FAU - Mazaheri, Zohreh
AU  - Mazaheri Z
AD  - Department of Anatomy, School of Medicine, Tarbiat Modares University, Tehran, 
      Iran.
FAU - Sobhani, Aligholi
AU  - Sobhani A
AD  - Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, 
      Tehran, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Avicenna J Med Biotechnol
JT  - Avicenna journal of medical biotechnology
JID - 101511065
PMC - PMC4224661
OTO - NOTNLM
OT  - Bone morphogenetic protein
OT  - Germ cells
OT  - Mesenchymal Stem Cells
OT  - Retinoic acid
EDAT- 2014/11/22 06:00
MHDA- 2014/11/22 06:01
PMCR- 2014/10/01
CRDT- 2014/11/22 06:00
PHST- 2014/03/08 00:00 [received]
PHST- 2014/06/20 00:00 [accepted]
PHST- 2014/11/22 06:00 [entrez]
PHST- 2014/11/22 06:00 [pubmed]
PHST- 2014/11/22 06:01 [medline]
PHST- 2014/10/01 00:00 [pmc-release]
AID - AJMB-6-218 [pii]
PST - ppublish
SO  - Avicenna J Med Biotechnol. 2014 Oct;6(4):218-27.