PMID- 25417716
OWN - NLM
STAT- MEDLINE
DCOM- 20150408
LR  - 20161125
IS  - 1742-4658 (Electronic)
IS  - 1742-464X (Linking)
VI  - 282
IP  - 3
DP  - 2015 Feb
TI  - MicroRNA biogenesis factor DRB1 is a phosphorylation target of mitogen activated 
      protein kinase MPK3 in both rice and Arabidopsis.
PG  - 521-36
LID - 10.1111/febs.13159 [doi]
AB  - MicroRNA (miRNA) biogenesis requires AtDRB1 (double-stranded RNA binding 
      protein)/HYL1 (Hyponastic Leaves1) protein for processing and maturation of miRNA 
      precursors. The AtDRB1/HYL1 protein associates with AtDCL1 (Dicer-Like1) and 
      accurately processes primary-miRNAs (pri-mRNAs) first to precursor-miRNAs 
      (pre-miRNAs) and finally to mature miRNAs. The dephosphorylation of AtDRB1/HYL1 
      protein is very important for the precise processing of miRNA precursors. The 
      monocot model crop plant Oryza sativa encodes four orthologues of AtDRB1/HYL1 
      protein, the only one encoded by Arabidopsis thaliana. The present study focuses 
      on the functionality of the O. sativa DRBs as the orthologues of AtDRB1/HYL1 by 
      using RNA binding assays and in planta protein-protein interaction analysis. 
      Further, mitogen-activated protein kinase MPK3 is established as the kinase 
      phosphorylating DRB1 protein in both the model plants, O. sativa and Arabidopsis. 
      MicroRNA microarray analysis in atmpk3 and atmpk6 mutants indicate the importance 
      of AtMPK3 in maintaining the level of miRNAs in the plant.
CI  - (c) 2014 FEBS.
FAU - Raghuram, Badmi
AU  - Raghuram B
AD  - National Institute of Plant Genome Research, New Delhi, India.
FAU - Sheikh, Arsheed H
AU  - Sheikh AH
FAU - Rustagi, Yashika
AU  - Rustagi Y
FAU - Sinha, Alok K
AU  - Sinha AK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141216
PL  - England
TA  - FEBS J
JT  - The FEBS journal
JID - 101229646
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (HYL1 protein, Arabidopsis)
RN  - 0 (MicroRNAs)
RN  - 0 (Plant Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - EC 2.7.11.24 (AtMPK3 protein, Arabidopsis)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
SB  - IM
MH  - Arabidopsis/*enzymology/*metabolism
MH  - Arabidopsis Proteins/*metabolism
MH  - MicroRNAs/metabolism
MH  - Mitogen-Activated Protein Kinase Kinases/*metabolism
MH  - Oryza/*enzymology/*metabolism
MH  - Phosphorylation
MH  - Plant Proteins/*metabolism
MH  - Protein Binding
MH  - RNA-Binding Proteins/*metabolism
OTO - NOTNLM
OT  - Arabidopsis
OT  - MAP kinase
OT  - double stranded RNA binding proteins
OT  - miRNA biogenesis
OT  - miRNA microarray
OT  - protein-protein interaction
OT  - rice
EDAT- 2014/11/25 06:00
MHDA- 2015/04/09 06:00
CRDT- 2014/11/25 06:00
PHST- 2014/07/14 00:00 [received]
PHST- 2014/11/07 00:00 [revised]
PHST- 2014/11/20 00:00 [accepted]
PHST- 2014/11/25 06:00 [entrez]
PHST- 2014/11/25 06:00 [pubmed]
PHST- 2015/04/09 06:00 [medline]
AID - 10.1111/febs.13159 [doi]
PST - ppublish
SO  - FEBS J. 2015 Feb;282(3):521-36. doi: 10.1111/febs.13159. Epub 2014 Dec 16.