PMID- 2541955
OWN - NLM
STAT- MEDLINE
DCOM- 19890707
LR  - 20190824
IS  - 0305-1870 (Print)
IS  - 0305-1870 (Linking)
VI  - 16
IP  - 3
DP  - 1989 Mar
TI  - Effect of colforsin on human neutrophil superoxide production and intracellular 
      calcium mobilization.
PG  - 199-209
AB  - 1. The effects of dibutyryl cyclic AMP (cAMP), isoproterenol and colforsin 
      (forskolin) were evaluated on respiratory burst in human polymorphonuclear 
      neutrophils (PMN). 2. Dibutyryl cAMP showed a dose-dependent inhibition of 
      n-formyl-methionyl-leucyl-phenylalanine (FMLP)-induced superoxide production by 
      human PMN. 3. Administration of isoproterenol induced a dose-dependent inhibition 
      of FMLP-induced superoxide production by human PMN, and the inhibition was 
      blocked by propranolol. 4. Administration of colforsin induced a dose-dependent 
      inhibition of FMLP-induced superoxide production by human PMN, and the inhibition 
      could not be blocked by propranolol. 5. Incubation with colforsin caused a 
      significant increase in the cAMP level in human PMN. 6. Pretreatment with 
      colforsin caused a dose-dependent inhibition in the elevation of intracellular 
      free calcium (monitored by fura-2 fluorescence), which was observed in human PMN 
      stimulated with FMLP. 7. These results suggest that cAMP is an inhibitory factor 
      of superoxide production and intracellular calcium mobilization in human PMN 
      stimulated with FMLP.
FAU - Furui, H
AU  - Furui H
AD  - 2nd Department of Internal Medicine, Nagoya University School of Medicine, Japan.
FAU - Suzuki, K
AU  - Suzuki K
FAU - Takagi, K
AU  - Takagi K
FAU - Satake, T
AU  - Satake T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Clin Exp Pharmacol Physiol
JT  - Clinical and experimental pharmacology & physiology
JID - 0425076
RN  - 11062-77-4 (Superoxides)
RN  - 1F7A44V6OU (Colforsin)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - 9Y8NXQ24VQ (Propranolol)
RN  - E0399OZS9N (Cyclic AMP)
RN  - L628TT009W (Isoproterenol)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adult
MH  - Bucladesine/pharmacology
MH  - Calcium/*blood/metabolism
MH  - Colforsin/*pharmacology
MH  - Cyclic AMP/blood
MH  - Cytosol/drug effects/metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Isoproterenol/pharmacology
MH  - Male
MH  - Neutrophils/drug effects/*metabolism
MH  - Propranolol/pharmacology
MH  - Superoxides/*blood
MH  - Time Factors
EDAT- 1989/03/01 00:00
MHDA- 1989/03/01 00:01
CRDT- 1989/03/01 00:00
PHST- 1989/03/01 00:00 [pubmed]
PHST- 1989/03/01 00:01 [medline]
PHST- 1989/03/01 00:00 [entrez]
AID - 10.1111/j.1440-1681.1989.tb01545.x [doi]
PST - ppublish
SO  - Clin Exp Pharmacol Physiol. 1989 Mar;16(3):199-209. doi: 
      10.1111/j.1440-1681.1989.tb01545.x.