PMID- 2542181
OWN - NLM
STAT- MEDLINE
DCOM- 19890710
LR  - 20180215
IS  - 0300-5526 (Print)
IS  - 0300-5526 (Linking)
VI  - 30
IP  - 2
DP  - 1989
TI  - Positive and negative modification of dexamethasone-mediated MMTV gp52 release by 
      progesterone pretreatment of tumor cells.
PG  - 111-6
AB  - Murine mammary tumor cells (C3H Mm5mt/cl) were grown in charcoal-stripped serum 
      to examine the effects of hormonal treatments on the expression and release of 
      the mouse mammary tumor virus (MMTV) envelope glycoprotein (gp52). The ability of 
      progesterone to modify extracellular levels of soluble and virion-associated MMTV 
      gp52 was tested singly and as a pretreatment prior to dexamethasone (DXS) 
      stimulation. Single treatments with DXS or progesterone demonstrated that DXS was 
      substantially more effective at elevating gp52 than progesterone; however, a 
      small but significant increase in gp52 levels was noted after 72 h of 
      progesterone treatment. Progesterone pretreatments at 10- to 100-fold excess of 
      DXS altered subsequent DXS-stimulated gp52 levels. Progesterone effectively 
      antagonized DXS and reduced gp52 levels to those of controls but this reduction 
      was accomplished only at the lowest concentrations of DXS (10(-8) M) and shortest 
      treatment interval (24h). While some pretreatment protocols of varied dose 
      slightly reduced gp52 levels at a point in time after treatment, the majority of 
      progesterone pretreatments at 10(-7), 10(-6), and 10(-5) M resulted in MMTV gp52 
      levels which were equal to and in many cases significantly greater than those 
      obtained with DXS alone. The ability to augment DXS-stimulated gp52 levels with 
      progesterone pretreatment suggests that, under certain hormonal conditions, gp52 
      expression and release from mouse cells may be progesterone-dependent as well as 
      glucocorticoid-dependent.
FAU - Ritzi, E M
AU  - Ritzi EM
AD  - Department of Microbiology, Texas Tech University Health Sciences Center, Lubbock 
      79430.
FAU - Walthall, E B
AU  - Walthall EB
LA  - eng
GR  - CA 28305-01/CA/NCI NIH HHS/United States
GR  - CA 34711-02/CA/NCI NIH HHS/United States
GR  - CA 38355-01/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Intervirology
JT  - Intervirology
JID - 0364265
RN  - 0 (Antigens, Viral, Tumor)
RN  - 0 (glycoprotein 52 antigen, Mouse mammary tumor virus)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Antigens, Viral, Tumor/*analysis
MH  - Dexamethasone/*pharmacology
MH  - Mammary Neoplasms, Experimental/*microbiology
MH  - Mammary Tumor Virus, Mouse/*immunology
MH  - Progesterone/*pharmacology
MH  - Tumor Cells, Cultured
EDAT- 1989/01/01 00:00
MHDA- 1989/01/01 00:01
CRDT- 1989/01/01 00:00
PHST- 1989/01/01 00:00 [pubmed]
PHST- 1989/01/01 00:01 [medline]
PHST- 1989/01/01 00:00 [entrez]
AID - 10.1159/000150082 [doi]
PST - ppublish
SO  - Intervirology. 1989;30(2):111-6. doi: 10.1159/000150082.