PMID- 25422556
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141125
LR  - 20200930
IS  - 0973-1296 (Print)
IS  - 0976-4062 (Electronic)
IS  - 0973-1296 (Linking)
VI  - 10
IP  - 40
DP  - 2014 Oct
TI  - Protective effects of methanolic extract form fruits of Lycium ruthenicum Murr on 
      2,2'-azobis (2-amidinopropane) dihydrochloride-induced oxidative stress in 
      LLC-PK1 cells.
PG  - 522-8
LID - 10.4103/0973-1296.141790 [doi]
AB  - BACKGROUND: Fruits of Lycium ruthenicum Murr is a health food and also used as a 
      folk to treat heart disease, abnormal menstruation and menopause in Tibetan, 
      China. However; whether L. ruthenicum Murr fruits methanolic extracts (LFME) 
      protect LLC-PK1 porcine renal tubules cells from AAPH-induced oxidative damage 
      has not been investigated. OBJECTIVE: To investigate the protective effects of L. 
      ruthenicum Murr fruits methanolic extracts (LFME) against 2, 2'- azobis 
      (2-amidinopropane) dihydrochloride (AAPH)-induced oxidative damage in renal 
      proximal tubule LLC-PK1 cells. MATERIALS AND METHODS: LLC-PK1 cells were 
      co-incubated with AAPH (1mM) and different concentrations of LFMW together for 24 
      h. Cell viability was determined by MTT assay. Total intercellular reactive 
      oxygen species (ROS) levels and lipid peroxidation were measured using a 
      fluorescent probe 2', 7'-dichlorfluorescein-diacetate (DCFH-DA) and the TBA 
      reactive substance (TBARS) assay, respectively. The endogenous antioxidant 
      enzymes including catalase (CAT), superoxide dismutase (SOD), glutathione 
      peroxidase (GSH-px) and intercellular glutathione (GSH) levels were determined 
      using commercial assay kits according to the manufacturer's instructions. 
      RESULTS: LFME did not show a significant cytotoxic effect and increased the 
      viability of LLC-PK1 cells in a concentration-dependent manner. LFME also 
      decreased the total intercellular levels of ROS, reduced lipid peroxidation and 
      increased the GSH levels as well as the activities of endogenous antioxidant 
      enzymes to protect LLC-PK1 cells against AAPH-induced oxidative damage. 
      CONCLUSION: The results from the present study indicated that LFME is an 
      effective ROS scavenger to protect LLC-PK1 cells against AAPH-induced oxidative 
      damage through decreasing ROS generation, reducing lipid peroxidation and 
      up-regulation of endogenous GSH levels and antioxidant enzymes.
FAU - Song, Jia-Le
AU  - Song JL
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Guilin Medical 
      University, Guangxi 541004, People's Republic of China ; Department of Food 
      Science and Nutrition, Pusan National University, Busan 609-735, South Korea.
FAU - Gao, Yang
AU  - Gao Y
AD  - Department of Pharmacy, Northern Jiangsu People's Hospital Affiliated to Yangzhou 
      University (Clinical Medical College of Yangzhou University), Yangzhou, Jiangsu 
      225001, People's Republic of China.
FAU - Xu, Jianguo
AU  - Xu J
AD  - Department of Pharmacy, Northern Jiangsu People's Hospital Affiliated to Yangzhou 
      University (Clinical Medical College of Yangzhou University), Yangzhou, Jiangsu 
      225001, People's Republic of China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Pharmacogn Mag
JT  - Pharmacognosy magazine
JID - 101300403
PMC - PMC4239733
OTO - NOTNLM
OT  - Antioxidant enzymes
OT  - LLC-PK1 cells
OT  - Lycium ruthenicum Murr
OT  - oxidative stress
COIS- Conflict of Interest: None declared.
EDAT- 2014/11/26 06:00
MHDA- 2014/11/26 06:01
PMCR- 2014/10/01
CRDT- 2014/11/26 06:00
PHST- 2013/10/26 00:00 [received]
PHST- 2013/12/06 00:00 [revised]
PHST- 2014/09/26 00:00 [accepted]
PHST- 2014/11/26 06:00 [entrez]
PHST- 2014/11/26 06:00 [pubmed]
PHST- 2014/11/26 06:01 [medline]
PHST- 2014/10/01 00:00 [pmc-release]
AID - PM-10-522 [pii]
AID - 10.4103/0973-1296.141790 [doi]
PST - ppublish
SO  - Pharmacogn Mag. 2014 Oct;10(40):522-8. doi: 10.4103/0973-1296.141790.