PMID- 25423836
OWN - NLM
STAT- MEDLINE
DCOM- 20141223
LR  - 20200508
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 39
IP  - 15
DP  - 2014 Aug
TI  - [Catalpol protect diabetic vascular endothelial function by inhibiting NADPH 
      oxidase].
PG  - 2936-41
AB  - The aim of the present study was to evaluate the protective effect of catalpol on 
      vascular endothelial function in STZ-induced type 2 diabetes mellitus (T2DM) 
      rats. 40 high-fat diet with STZ-induced diabetes rats were randomly divided into 
      model group, catalpol low-dose, middle-dose and high-dose group (10, 50, 100 mg x 
      kg(-1) x d(-1)), 10 normal Wistar rats were used as the normal group. The normal 
      and model groups were given an equivalent amount of saline. All reagents were 
      administered by oral gavage for 6 weeks. After 6 weeks, blood glucose and lipids 
      were detected by an automatic biochemical analyzer. The endothelium-dependent 
      vasodilation response of thoracic aortar was detected. The pathological changes 
      of the thoracic aorta were observed by HE staining. Ser- um nitric oxide (NO), 
      8-iso prostaglandin F2alpha (8-iso-PGF2alpha) and superoxide dismutase (SOD) were 
      detected by ELISA. Reactive oxygen species (ROS) level of thoracic aorta was 
      detected by fluorescence method. The expression of Nox4 and p22phox mRNA and 
      protein in aortic tissue were detected by RT-PCR and Western-blot respectively. 
      After catalpol treatment, endothelial damage of thoracic aorta was attenuated 
      significantly; ROS level of thoracic aorta and serum level of 8-iso-PGF2alpha were 
      decreased significantly; serum NO and SOD levels were remarkably elevated; 
      expression of Nox4, p22phox mRNA and protein in thoracic aorta were significantly 
      reduced (P < 0.05). Therefore, catalpol has protective effect on endothelial of 
      T2DM, its mechanism may be associated with the down-regulation of Nox4 and 
      p22phox expression, inhibiting oxidative stress reaction response.
FAU - Liu, Jiang-Yue
AU  - Liu JY
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Blood Glucose)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Iridoid Glucosides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 2415-24-9 (catalpol)
RN  - 27415-26-5 (8-epi-prostaglandin F2alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - B7IN85G1HY (Dinoprost)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.6.3.- (NADPH Oxidase 4)
RN  - EC 1.6.3.- (NADPH Oxidases)
RN  - EC 1.6.3.- (Nox4 protein, rat)
RN  - EC 1.6.3.1 (Cyba protein, rat)
SB  - IM
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - Diabetes Mellitus, Experimental/*pathology
MH  - Diabetes Mellitus, Type 2/*pathology
MH  - Dinoprost/analogs & derivatives/metabolism
MH  - Endothelium, Vascular/*drug effects/metabolism/*pathology
MH  - Enzyme Inhibitors/*pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Iridoid Glucosides/*pharmacology
MH  - Male
MH  - NADPH Oxidase 4
MH  - NADPH Oxidases/*antagonists & inhibitors/genetics/metabolism
MH  - Nitric Oxide/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Reactive Oxygen Species/metabolism
MH  - Superoxide Dismutase/metabolism
EDAT- 2014/11/27 06:00
MHDA- 2014/12/24 06:00
CRDT- 2014/11/27 06:00
PHST- 2014/11/27 06:00 [entrez]
PHST- 2014/11/27 06:00 [pubmed]
PHST- 2014/12/24 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2014 Aug;39(15):2936-41.