PMID- 2542416
OWN - NLM
STAT- MEDLINE
DCOM- 19890713
LR  - 20190723
IS  - 0022-202X (Print)
IS  - 0022-202X (Linking)
VI  - 92
IP  - 6
DP  - 1989 Jun
TI  - GTP-dependent hydrolysis of phosphatidylinositol-4,5-bisphosphate by soluble 
      phospholipase C from adult human epidermis.
PG  - 831-6
AB  - The regulation of soluble phosphoinositide-specific phospholipase C from adult 
      human epidermis by guanine nucleotide was investigated. In the presence of 
      physiologic concentrations of Ca++ (1 microM) and Mg++ (1.5 mM), neither 
      phosphatidylinositol (PI) nor phosphatidylinositol-4,5-bisphosphate (PIP2) were 
      appreciably hydrolyzed. Addition of guanosine-5'-triphosphate (GTP) or 
      guanosine-5'-O-(3-thiotriphosphate) (GTP-gamma-S) significantly stimulated 
      hydrolysis of PIP2, but not PI. Stimulation of PIP2 hydrolysis by GTP was 
      dose-dependent between 1-100 microM GTP. Other nucleoside triphosphates and 
      nucleotide analogues were unable to substitute for GTP or GTP-gamma-S. A 
      GTP-gamma-S-stimulated PIP2 hydrolysis was inhibited by 
      guanosine-5'-O-(2-thiodiphosphate (GDP-beta-S). The phospholipase C preparation 
      specifically bound [35S]GTP-gamma-S and this binding was also inhibited by 
      GDP-beta-S. In addition to a 41,000-dalton pertussis toxin substrate, the 
      phospholipase C preparation contained 3-4 GTP binding proteins with molecular 
      weights between 20,000-30,000. These data demonstrate that human epidermis 
      contains a soluble GTP-dependent phospholipase C activity that specifically 
      hydrolyzes PIP2 and suggest that this reaction is regulated by a GTP-binding 
      protein(s).
FAU - Fisher, G J
AU  - Fisher GJ
AD  - Department of Dermatology, University of Michigan Medical School, Ann Arbor 
      48109.
FAU - Baldassare, J J
AU  - Baldassare JJ
FAU - Voorhees, J J
AU  - Voorhees JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (Phosphatidylinositol 4,5-Diphosphate)
RN  - 0 (Phosphatidylinositols)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adult
MH  - Calcium/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Epidermis/*metabolism
MH  - GTP-Binding Proteins/analysis
MH  - Guanosine Triphosphate/metabolism/*pharmacology
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Hydrolysis
MH  - Phosphatidylinositol 4,5-Diphosphate
MH  - Phosphatidylinositols/*metabolism
MH  - Type C Phospholipases/*metabolism
EDAT- 1989/06/01 00:00
MHDA- 1989/06/01 00:01
CRDT- 1989/06/01 00:00
PHST- 1989/06/01 00:00 [pubmed]
PHST- 1989/06/01 00:01 [medline]
PHST- 1989/06/01 00:00 [entrez]
AID - S0022-202X(89)90321-7 [pii]
AID - 10.1111/1523-1747.ep12696846 [doi]
PST - ppublish
SO  - J Invest Dermatol. 1989 Jun;92(6):831-6. doi: 10.1111/1523-1747.ep12696846.