PMID- 25424467
OWN - NLM
STAT- MEDLINE
DCOM- 20160307
LR  - 20201209
IS  - 1756-0500 (Electronic)
IS  - 1756-0500 (Linking)
VI  - 7
DP  - 2014 Nov 25
TI  - Subtoxic levels of hydrogen peroxide induce brain-derived neurotrophic factor 
      expression to protect PC12 cells.
PG  - 840
LID - 10.1186/1756-0500-7-840 [doi]
LID - 840
AB  - BACKGROUND: Oxidative stress is one of the mechanisms underlying pathogenesis in 
      neurodegenerative diseases such as Alzheimer's disease. Generally, oxidative 
      stress represents cell toxicity; however, we recently found that oxidative stress 
      promotes the expression of growth factor progranulin (PGRN) in HT22 murine 
      hippocampus cells, thereby protecting the HT22 cells. In this study, we attempted 
      to clarify whether a similar system exists in the other neuronal cell model, rat 
      pheochromocytoma (PC12) cells. RESULTS: After confirming that high concentrations 
      of hydrogen peroxide (H2O2; 100-250 muM) initiate PC12 cell death, we analyzed 
      growth factor expressional changes after H2O2 treatment. We found, intriguingly, 
      that gene expression of brain-derived neurotrophic factor (BDNF), but not PGRN 
      was significantly induced by H2O2. Although little expression of the high 
      affinity BDNF receptor tropomyosin-related kinase TrkB was observed in PC12 
      cells, expression of low affinity neurotrophin receptor, p75NTR, was clearly 
      observed. This BDNF signaling appeared to contribute to PC12 cell protection, 
      since PC12 cell death was significantly attenuated by BDNF treatment. 
      CONCLUSIONS: Based on our results, we conclude that the induction of BDNF by 
      subtoxic levels of H2O2 and its signaling may have roles in PC12 cell protection.
FAU - Ogura, Yurina
AU  - Ogura Y
FAU - Sato, Kazunori
AU  - Sato K
FAU - Kawashima, Ken-Ichi
AU  - Kawashima K
FAU - Kobayashi, Nanako
AU  - Kobayashi N
FAU - Imura, Sei
AU  - Imura S
FAU - Fujino, Kotaro
AU  - Fujino K
FAU - Kawaguchi, Hideo
AU  - Kawaguchi H
FAU - Nedachi, Taku
AU  - Nedachi T
AD  - Department of Life Sciences, Graduate School of Life Sciences, Toyo University, 
      1-1-1 Izumino, Itakura-machi, Oura-gun, Gunma 374-0193, Japan. nedachi@toyo.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141125
PL  - England
TA  - BMC Res Notes
JT  - BMC research notes
JID - 101462768
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Grn protein, rat)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Progranulins)
RN  - 0 (Receptors, Growth Factor)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 136958-07-1 (Ngfr protein, rat)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/agonists/*genetics/metabolism/pharmacology
MH  - Cell Death/drug effects
MH  - Dose-Response Relationship, Drug
MH  - *Gene Expression Regulation
MH  - Hydrogen Peroxide/*pharmacology
MH  - Intercellular Signaling Peptides and Proteins/genetics/metabolism
MH  - Nerve Tissue Proteins
MH  - Oxidative Stress
MH  - PC12 Cells
MH  - Progranulins
MH  - Rats
MH  - Receptor, trkB/genetics/metabolism
MH  - Receptors, Growth Factor
MH  - Receptors, Nerve Growth Factor/genetics/metabolism
MH  - Signal Transduction
PMC - PMC4256810
EDAT- 2014/11/27 06:00
MHDA- 2016/03/08 06:00
PMCR- 2014/11/25
CRDT- 2014/11/27 06:00
PHST- 2014/07/08 00:00 [received]
PHST- 2014/11/18 00:00 [accepted]
PHST- 2014/11/27 06:00 [entrez]
PHST- 2014/11/27 06:00 [pubmed]
PHST- 2016/03/08 06:00 [medline]
PHST- 2014/11/25 00:00 [pmc-release]
AID - 1756-0500-7-840 [pii]
AID - 3364 [pii]
AID - 10.1186/1756-0500-7-840 [doi]
PST - epublish
SO  - BMC Res Notes. 2014 Nov 25;7:840. doi: 10.1186/1756-0500-7-840.