PMID- 25426063
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141126
LR  - 20220129
IS  - 1663-3563 (Print)
IS  - 1663-3563 (Electronic)
IS  - 1663-3563 (Linking)
VI  - 6
DP  - 2014
TI  - BDNF enhances spontaneous and activity-dependent neurotransmitter release at 
      excitatory terminals but not at inhibitory terminals in hippocampal neurons.
PG  - 27
LID - 10.3389/fnsyn.2014.00027 [doi]
LID - 27
AB  - Brain-derived neurotrophic factor (BDNF) is widely reported to enhance synaptic 
      vesicle (SV) exocytosis and neurotransmitter release. But it is still unclear 
      whether BDNF enhances SV recycling at excitatory terminals only, or at both 
      excitatory and inhibitory terminals. In the present study, in a direct comparison 
      using cultured rat hippocampal neurons, we demonstrate that BDNF enhances both 
      spontaneous and activity-dependent neurotransmitter release from excitatory 
      terminals, but not from inhibitory terminals. BDNF treatment for 5 min or 48 h 
      increased both spontaneous and activity-induced anti-synaptotagmin1 (SYT1) 
      antibody uptake at excitatory terminals marked with vGluT1. Conversely, BDNF 
      treatment did not enhance spontaneous or activity-induced uptake of anti-SYT1 
      antibodies in inhibitory terminals marked with vGAT. Time-lapse imaging of FM1-43 
      dye destaining in excitatory and inhibitory terminals visualized by post-hoc 
      immunostaining of vGluT1 and vGAT also showed the same result: The rate of 
      spontaneous and activity-induced destaining was increased by BDNF at excitatory 
      synapses, but not at inhibitory synapses. These data demonstrate that BDNF 
      enhances SV exocytosis in excitatory but not inhibitory terminals. Moreover, BDNF 
      enhanced evoked SV exocytosis, even if vesicles were loaded under spontaneous 
      vesicle recycling conditions. Thus, BDNF enhances both spontaneous and 
      activity-dependent neurotransmitter release on both short and long time-scales, 
      by the same mechanism.
FAU - Shinoda, Yo
AU  - Shinoda Y
AD  - Trans-synaptic Signaling Group, European Neuroscience Institute Goettingen, 
      Germany ; Department of Applied Biological Science, Faculty of Science and 
      Technology, Tokyo University of Science Chiba, Japan.
FAU - Ahmed, Saheeb
AU  - Ahmed S
AD  - Trans-synaptic Signaling Group, European Neuroscience Institute Goettingen, 
      Germany.
FAU - Ramachandran, Binu
AU  - Ramachandran B
AD  - Trans-synaptic Signaling Group, European Neuroscience Institute Goettingen, 
      Germany.
FAU - Bharat, Vinita
AU  - Bharat V
AD  - Trans-synaptic Signaling Group, European Neuroscience Institute Goettingen, 
      Germany.
FAU - Brockelt, David
AU  - Brockelt D
AD  - Trans-synaptic Signaling Group, European Neuroscience Institute Goettingen, 
      Germany.
FAU - Altas, Bekir
AU  - Altas B
AD  - Trans-synaptic Signaling Group, European Neuroscience Institute Goettingen, 
      Germany.
FAU - Dean, Camin
AU  - Dean C
AD  - Trans-synaptic Signaling Group, European Neuroscience Institute Goettingen, 
      Germany.
LA  - eng
GR  - 260916/ERC_/European Research Council/International
PT  - Journal Article
DEP - 20141110
PL  - Switzerland
TA  - Front Synaptic Neurosci
JT  - Frontiers in synaptic neuroscience
JID - 101548972
PMC - PMC4226143
OTO - NOTNLM
OT  - brain-derived neurotrophic factor
OT  - excitatory/inhibitory
OT  - exocytosis
OT  - hippocampal neurons
OT  - neurotransmitter release
EDAT- 2014/11/27 06:00
MHDA- 2014/11/27 06:01
PMCR- 2014/01/01
CRDT- 2014/11/27 06:00
PHST- 2014/09/13 00:00 [received]
PHST- 2014/10/21 00:00 [accepted]
PHST- 2014/11/27 06:00 [entrez]
PHST- 2014/11/27 06:00 [pubmed]
PHST- 2014/11/27 06:01 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - 10.3389/fnsyn.2014.00027 [doi]
PST - epublish
SO  - Front Synaptic Neurosci. 2014 Nov 10;6:27. doi: 10.3389/fnsyn.2014.00027. 
      eCollection 2014.