PMID- 25428555
OWN - NLM
STAT- MEDLINE
DCOM- 20150928
LR  - 20181113
IS  - 1471-2229 (Electronic)
IS  - 1471-2229 (Linking)
VI  - 14
DP  - 2014 Nov 27
TI  - Patatin-related phospholipase pPLAIIIdelta influences auxin-responsive cell 
      morphology and organ size in Arabidopsis and Brassica napus.
PG  - 332
LID - 10.1186/s12870-014-0332-1 [doi]
LID - 332
AB  - BACKGROUND: The members of the patatin-related phospholipase subfamily III 
      (pPLAIIIs) have been implicated in the auxin response. However, it is not clear 
      whether and how these genes affect plant and cell morphogenesis. Here, we studied 
      the roles of the patatin-related phospholipase pPLAIIIdelta in auxin-responsive cell 
      morphology and organ size in Arabidopsis and Brassica napus. RESULTS: We show 
      that overexpression of pPLAIIIdelta inhibited longitudinal growth but promoted 
      transverse growth in most organs of Arabidopsis and Brassica napus. Compared to 
      wild-type plants, pPLAIIIdelta-KO plants exhibited enhanced cell elongation in 
      hypocotyls, and pPLAIIIdelta-OE plants displayed broadened radial cell growth of 
      hypocotyl and reduced leaf pavement cell polarity. For the hypocotyl phenotype in 
      pPLAIIIdelta mutants, which resembles the "triple response" to ethylene, we examined 
      the expression of the ACS and ACO genes involved in ethylene biosynthesis and 
      found that ACS4 and ACS5 were up-regulated by 2.5-fold on average in two OE lines 
      compared with WT plants. The endogenous auxin distribution was disturbed in 
      plants with altered pPLAIIIdelta expression. pPLAIIIdelta-OE and KO plants exhibited 
      different sensitivities to indole-3-acetic acid-promoted hypocotyl elongation in 
      both light and dark conditions. Gene expression analysis of auxin-induced genes 
      in the dark showed that OE plants maintained a higher auxin response compared 
      with WT and KO plants after treatment with 1 muM IAA for 12 h. Following treatment 
      with 10 muM IAA for 30 min in the light, early auxin-induced genes were 
      significantly up-regulated in two OE plant lines. CONCLUSIONS: These data suggest 
      that the PLAIIIdelta gene plays an important role in cell morphology and organ size 
      through its involvement in the regulation of auxin distribution in plants.
FAU - Dong, Yanni
AU  - Dong Y
FAU - Li, Maoyin
AU  - Li M
FAU - Zhang, Peng
AU  - Zhang P
FAU - Wang, Xuemin
AU  - Wang X
FAU - Fan, Chuchuan
AU  - Fan C
FAU - Zhou, Yongming
AU  - Zhou Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141127
PL  - England
TA  - BMC Plant Biol
JT  - BMC plant biology
JID - 100967807
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Indoleacetic Acids)
RN  - 0 (Plant Growth Regulators)
RN  - 0 (Plant Proteins)
RN  - EC 3.1.1.- (pPLAIIIdelta protein, Arabidopsis)
RN  - EC 3.1.1.32 (Phospholipases A)
SB  - IM
MH  - Arabidopsis/*enzymology/genetics/growth & development
MH  - Arabidopsis Proteins/genetics/metabolism
MH  - Brassica napus/*enzymology/genetics/growth & development
MH  - Cell Enlargement
MH  - *Gene Expression Regulation, Plant
MH  - Indoleacetic Acids/*metabolism
MH  - Mutation
MH  - Phospholipases A/genetics/*metabolism
MH  - Plant Growth Regulators/*metabolism
MH  - Plant Proteins/genetics/*metabolism
MH  - Signal Transduction
PMC - PMC4253999
EDAT- 2014/11/28 06:00
MHDA- 2015/09/29 06:00
PMCR- 2014/11/27
CRDT- 2014/11/28 06:00
PHST- 2014/09/20 00:00 [received]
PHST- 2014/11/11 00:00 [accepted]
PHST- 2014/11/28 06:00 [entrez]
PHST- 2014/11/28 06:00 [pubmed]
PHST- 2015/09/29 06:00 [medline]
PHST- 2014/11/27 00:00 [pmc-release]
AID - s12870-014-0332-1 [pii]
AID - 332 [pii]
AID - 10.1186/s12870-014-0332-1 [doi]
PST - epublish
SO  - BMC Plant Biol. 2014 Nov 27;14:332. doi: 10.1186/s12870-014-0332-1.