PMID- 25428773
OWN - NLM
STAT- MEDLINE
DCOM- 20150522
LR  - 20161125
IS  - 1554-527X (Electronic)
IS  - 0736-0266 (Linking)
VI  - 33
IP  - 4
DP  - 2015 Apr
TI  - Effect of tenascin-C on the repair of full-thickness osteochondral defects of 
      articular cartilage in rabbits.
PG  - 563-71
LID - 10.1002/jor.22794 [doi]
AB  - The purpose of this study was to examine the effect of tenascin-C (TNC) on the 
      repair of full-thickness osteochondral defects of articular cartilage in vivo. We 
      used a gellan-gellan-sulfate sponge (Gellan-GS) to maintain a TNC-rich 
      environment in the cartilage defects. We implanted Gellan-GS soaked in PBS only 
      (Group 1), Gellan-GS soaked in 10 microg/ml of TNC (Group 2), and Gellan-GS soaked in 
      100 microg/ml of TNC (Group 3) into a full-thickness osteochondral defect of the 
      patellar groove of rabbits. The defect area was examined grossly and 
      histologically 4-12 weeks after surgery. Sections of synovium were also 
      immunohistochemically investigated. Histologically as well as macroscopically, 
      the defects in Group 2 showed better repair than the other groups at 8 and 12 
      weeks after surgery. Inflammation of the synovium tended to diminish over time in 
      all groups, and the degree of synovitis was the same for all three groups at each 
      time point. In conclusion, Gellan-GS soaked in TNC can be used as a novel 
      scaffold for the repair of articular cartilage defects. This study also indicates 
      that TNC promotes the repair of full-thickness osteochondral defects in vivo.
CI  - (c) 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
FAU - Ikemura, Shigeto
AU  - Ikemura S
AD  - Department of Orthopaedic Surgery, Mie University Graduate School of Medicine, 
      Mie, Japan.
FAU - Hasegawa, Masahiro
AU  - Hasegawa M
FAU - Iino, Takahiro
AU  - Iino T
FAU - Miyamoto, Keiichi
AU  - Miyamoto K
FAU - Imanaka-Yoshida, Kyoko
AU  - Imanaka-Yoshida K
FAU - Yoshida, Toshimichi
AU  - Yoshida T
FAU - Sudo, Akihiro
AU  - Sudo A
LA  - eng
PT  - Journal Article
DEP - 20150212
PL  - United States
TA  - J Orthop Res
JT  - Journal of orthopaedic research : official publication of the Orthopaedic 
      Research Society
JID - 8404726
RN  - 0 (Delayed-Action Preparations)
RN  - 0 (Polysaccharides)
RN  - 0 (Polysaccharides, Bacterial)
RN  - 0 (Sulfuric Acid Esters)
RN  - 0 (Tenascin)
RN  - 0 (gellan sulfate)
RN  - 7593U09I4D (gellan gum)
SB  - IM
MH  - Animals
MH  - Cartilage, Articular/*drug effects/*injuries/pathology
MH  - Delayed-Action Preparations
MH  - Disease Models, Animal
MH  - Female
MH  - Patella/injuries
MH  - Polysaccharides/pharmacology
MH  - Polysaccharides, Bacterial/pharmacology
MH  - Rabbits
MH  - Sulfuric Acid Esters/pharmacology
MH  - Surgical Sponges
MH  - Synovial Membrane/pathology
MH  - Tenascin/*pharmacology
MH  - Wound Healing/*drug effects
MH  - Wounds, Penetrating/*drug therapy/pathology/physiopathology
OTO - NOTNLM
OT  - animal model
OT  - cartilage repair
OT  - gellan-gellan-sulfate
OT  - tenascin-C
EDAT- 2014/11/28 06:00
MHDA- 2015/05/23 06:00
CRDT- 2014/11/28 06:00
PHST- 2014/08/13 00:00 [received]
PHST- 2014/11/24 00:00 [accepted]
PHST- 2014/11/28 06:00 [entrez]
PHST- 2014/11/28 06:00 [pubmed]
PHST- 2015/05/23 06:00 [medline]
AID - 10.1002/jor.22794 [doi]
PST - ppublish
SO  - J Orthop Res. 2015 Apr;33(4):563-71. doi: 10.1002/jor.22794. Epub 2015 Feb 12.