PMID- 25430081
OWN - NLM
STAT- MEDLINE
DCOM- 20150825
LR  - 20181113
IS  - 1865-3774 (Electronic)
IS  - 0925-5710 (Linking)
VI  - 101
IP  - 1
DP  - 2015 Jan
TI  - Clonal origin and evolution of myelodysplastic syndrome analyzed by dysplastic 
      morphology and fluorescence in situ hybridization.
PG  - 58-66
LID - 10.1007/s12185-014-1700-1 [doi]
AB  - Myelodysplastic syndromes (MDS) are clonal disorders of hematopoietic 
      stem/progenitor cells. As bone marrow cells are extremely diverse in these 
      disorders, the origin and evolution of MDS clones are difficult to identify and 
      trace. Cellular dysplasia is a distinct morphologic feature; however, whether the 
      dysplastic cells represent abnormal clones or only nonspecific superficial 
      phenomena remains to be clarified. To address this question, 97 patients were 
      examined for dysplasia features, among them bone marrow slides of 16 patients 
      with chromosomal abnormalities were subjected to fluorescence in situ 
      hybridization (FISH) to determine the karyotype of these dysplastic cells. 
      Furthermore, the emerging frequencies of abnormal karyotypes in various 
      differentiated stages of each lineage were also evaluated by a combination of 
      morphological evaluation and FISH karyotyping. Our results indicate that the 
      overall percentage of dysplastic cells does not differ significantly among the 
      WHO subtypes, while the megakaryoid lineage presents the most frequent dysplasia 
      in all subtypes. A positive correlation between dysplastic cells and 
      FISH-detectable abnormal clones was observed, but the dysplastic morphology was 
      not a specific feature of FISH-detectable abnormal clones. FISH-detectable 
      abnormal clones can differentiate into mature granulocytes and erythrocytes, in 
      coexistence with cells originating from the normal clones.
FAU - Fu, Chun-Mei
AU  - Fu CM
AD  - Jiangsu Institute of Hematology, The first Affiliated Hospital of Soochow 
      University, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, 
      188 Shizi Street, Suzhou, 215006, Jiangsu, People's Republic of China.
FAU - Chen, Zi-Xing
AU  - Chen ZX
FAU - Liu, Dan-Dan
AU  - Liu DD
FAU - Zhang, Jun
AU  - Zhang J
FAU - Pan, Jin-Lan
AU  - Pan JL
FAU - Liang, Jian-Ying
AU  - Liang JY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141128
PL  - Japan
TA  - Int J Hematol
JT  - International journal of hematology
JID - 9111627
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bone Marrow/*pathology
MH  - Bone Marrow Cells/metabolism/pathology
MH  - Cell Lineage/genetics
MH  - Chromosome Aberrations
MH  - Clonal Evolution/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/diagnosis/*genetics/*pathology
MH  - Young Adult
EDAT- 2014/11/29 06:00
MHDA- 2015/08/26 06:00
CRDT- 2014/11/29 06:00
PHST- 2013/12/31 00:00 [received]
PHST- 2014/11/11 00:00 [accepted]
PHST- 2014/10/29 00:00 [revised]
PHST- 2014/11/29 06:00 [entrez]
PHST- 2014/11/29 06:00 [pubmed]
PHST- 2015/08/26 06:00 [medline]
AID - 10.1007/s12185-014-1700-1 [doi]
PST - ppublish
SO  - Int J Hematol. 2015 Jan;101(1):58-66. doi: 10.1007/s12185-014-1700-1. Epub 2014 
      Nov 28.