PMID- 25430440 OWN - NLM STAT- MEDLINE DCOM- 20150825 LR - 20161125 IS - 2542-5641 (Electronic) IS - 0366-6999 (Linking) VI - 127 IP - 23 DP - 2014 TI - Hippophae rhamnoides L. leaves extract enhances cell proliferation and neuroblast differentiation through upregulation of intrinsic factors in the dentate gyrus of the aged gerbil. PG - 4006-11 AB - BACKGROUND: Hippophae rhamnoides L. (HL) exerts antioxidant activities against various oxidative stress conditions. In this study, we investigated effects of extract from HL leaves (HLE) on cell proliferation and neuroblast differentiation in the subgranular zone (SGZ) of the dentate gyrus (DG) of aged gerbils. METHODS: Aged gerbils (24 months) were divided into vehicle (saline)-treated- and HLE-treated-groups. The vehicle and HLE were orally administered with 200 mg/kg once a day for 20 days before sacrifice. Cell proliferation and neuroblast differentiation were examined in the DG using Ki67 and doublecortin (DCX), respectively. We also observed changes in immunoreactivities of superoxide dismutase 1 (SOD1) and superoxide dismutase 2 (SOD2), brain-derived neurotrophic factor (BDNF), and phospho-glycogen synthase kinase-3-beta (p-GSK-3beta) to examine their relation with neurogenesis using immunohistochemistry. RESULTS: The administration of HLE significantly increased the number of Ki67-positive cells and DCX-positive neuroblasts with well-developed processes in the SGZ of the DG of the HLE-treated-group. In addition, immunoreactivities of SOD1, SOD2, BDNF, and p-GSK-3beta were significantly increased in granule and polymorphic cells of the DG in the HLE-treated-group compared with those in the vehicle-treated-group. CONCLUSIONS: HLE treatment significantly increased cell proliferation and neuroblast differentiation, showing that immunoreactivities of SOD1, SOD2, BDNF, and p-GSK-3beta were significantly increased in the DG. These indicate that increased neuroblast differentiation neurogenesis may be closely related to upregulation of SOD1, SOD2, BDNF, and p-GSK-3beta in aged gerbils. FAU - Ahn, Ji Hyeon AU - Ahn JH AD - Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea. FAU - Chen, Bai Hui AU - Chen BH AD - Department of Physiology, College of Medicine, Hallym University, Chuncheon 200-702, South Korea. FAU - Park, Joon Ha AU - Park JH AD - Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea. FAU - Kim, In Hye AU - Kim IH AD - Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea. FAU - Cho, Jeong-Hwi AU - Cho JH AD - Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea. FAU - Lee, Jae-Chul AU - Lee JC AD - Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea. FAU - Yan, Bing Chun AU - Yan BC AD - Institute of Integrative Traditional & Western Medicine, Medical College, Yangzhou University, Yangzhou, Jiangsu 225001, China. FAU - Choi, Jung Hoon AU - Choi JH AD - Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, South Korea. FAU - Hwang, In Koo AU - Hwang IK AD - Department of Anatomy and Cell Biology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea. FAU - Park, Ju-Hee AU - Park JH AD - Samsung Herb Medicine Company Limited, 304 BIO-1dong ChunCheon Biotechnology Innovation Center, Chuncheon 200-957, South Korea. FAU - Han, Sang-No AU - Han SN AD - ChunCheon Biotechnology Innovation Center, Chuncheon 200-957, South Korea. FAU - Lee, Yun Lyul AU - Lee YL AD - Department of Physiology, College of Medicine, Hallym University, Chuncheon 200-702, South Korea. FAU - Kim, Myong Jo AU - Kim MJ AD - Oriental Bio-herb Research Institute, Kangwon National University, Chuncheon 200-701, South Korea. Email: kimmjo@kangwon.ac.kr. FAU - Won, Moo-Ho AU - Won MH AD - Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea. Email: mhwon@kangwon.ac.kr. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - China TA - Chin Med J (Engl) JT - Chinese medical journal JID - 7513795 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 9008-12-2 (Intrinsic Factor) RN - EC 1.15.1.1 (Superoxide Dismutase) RN - EC 1.15.1.1 (Superoxide Dismutase-1) RN - EC 1.15.1.1 (superoxide dismutase 2) RN - EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta) RN - EC 2.7.11.26 (Glycogen Synthase Kinase 3) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/metabolism MH - Cell Differentiation/drug effects MH - Cell Proliferation/drug effects MH - Dentate Gyrus/*drug effects/*metabolism MH - Gerbillinae MH - Glycogen Synthase Kinase 3/metabolism MH - Glycogen Synthase Kinase 3 beta MH - Hippophae/*drug effects/*metabolism MH - Immunohistochemistry MH - Intrinsic Factor/*metabolism MH - Male MH - Neurogenesis/drug effects MH - Superoxide Dismutase/metabolism MH - Superoxide Dismutase-1 EDAT- 2014/11/29 06:00 MHDA- 2015/08/26 06:00 CRDT- 2014/11/29 06:00 PHST- 2014/11/29 06:00 [entrez] PHST- 2014/11/29 06:00 [pubmed] PHST- 2015/08/26 06:00 [medline] PST - ppublish SO - Chin Med J (Engl). 2014;127(23):4006-11.