PMID- 25431067
OWN - NLM
STAT- MEDLINE
DCOM- 20150708
LR  - 20141128
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1254
DP  - 2015
TI  - In vitro oxygen-glucose deprivation to study ischemic cell death.
PG  - 197-210
LID - 10.1007/978-1-4939-2152-2_15 [doi]
AB  - Oxygen-glucose deprivation (OGD ) is widely used as an in vitro model for stroke, 
      showing similarities with the in vivo models of brain ischemia. In order to 
      perform OGD, cell or tissue cultures, such as primary neurons or organotypic 
      slices, and acutely prepared tissue slices are usually incubated in a 
      glucose-free medium under a deoxygenated atmosphere, for example in a hypoxic 
      chamber. Here, we describe the step-by-step procedure to expose cultures and 
      acute slices to OGD, focusing on the most suitable methods for assessing cellular 
      death and/or viability. OGD is a simple yet highly useful technique, not only for 
      the elucidation of the role of key cellular and molecular mechanisms underlying 
      brain ischemia, but also for the development of novel neuroprotective strategies.
FAU - Tasca, Carla I
AU  - Tasca CI
AD  - Departamento de Bioquimica, Centro de Ciencias Biologicas, Universidade Federal 
      de Santa Catarina, Campus Universitario - Trindade 88040-900, Florianopolis, SC, 
      Brazil.
FAU - Dal-Cim, Tharine
AU  - Dal-Cim T
FAU - Cimarosti, Helena
AU  - Cimarosti H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - IY9XDZ35W2 (Glucose)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Brain Ischemia/*pathology
MH  - Cell Death/*genetics
MH  - Glucose/metabolism
MH  - Hippocampus/metabolism
MH  - Humans
MH  - Molecular Biology/methods
MH  - Neurons/metabolism/*pathology
MH  - Oxygen/metabolism
EDAT- 2014/11/29 06:00
MHDA- 2015/07/15 06:00
CRDT- 2014/11/29 06:00
PHST- 2014/11/29 06:00 [entrez]
PHST- 2014/11/29 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - 10.1007/978-1-4939-2152-2_15 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2015;1254:197-210. doi: 10.1007/978-1-4939-2152-2_15.