PMID- 25431121
OWN - NLM
STAT- MEDLINE
DCOM- 20160107
LR  - 20191210
IS  - 1099-1565 (Electronic)
IS  - 0958-0344 (Linking)
VI  - 26
IP  - 2
DP  - 2015 Mar-Apr
TI  - Development and validation of liquid chromatography combined with tandem mass 
      spectrometry methods for the quantitation of simalikalactone E in extracts of 
      Quassia amara L. and in mouse blood.
PG  - 111-8
LID - 10.1002/pca.2542 [doi]
AB  - INTRODUCTION: Simalikalactone E (SkE) from Quassia amara, has been proved to be a 
      valuable anti-malarial and anti-cancer compound. As SkE is very scarce, methods 
      of quantitation are needed in order to optimise its isolation process and to 
      determine pharmacokinetic data. OBJECTIVE: To validate methods using liquid 
      chromatography coupled to mass spectrometry for the quantitation of SkE in plant 
      extracts and in biological fluids. METHODS: High- and ultrahigh-performance 
      liquid chromatography (UHPLC) coupled to ion trap mass spectrometry (MS) with 
      single ion monitoring detection and to triple quadrupole-linear ion trap tandem 
      mass spectrometry with multiple reaction monitoring detection methods were 
      developed. Validation procedure was realised according to the International 
      Conference on Harmonisation guideline. Methanol extracts of dried Quassia amara 
      leaves, and mouse-blood samples obtained after various routes of administration, 
      were analysed for SkE. RESULTS: Methods were validated and gave similar results 
      regarding the content of SkE expressed per kilogram of dry leaves in the 
      traditional decoction (160 +/- 12 mg/kg) and in the methanol extract 
      (93 +/- 2 mg/kg). The recovery of the analyte from mouse blood ranged from 80.7 to 
      119.8%. Simalikalactone E was only detected using UHPLC-MS/MS (0.2 +/- 0.03 mg/L) 
      in mouse blood after intravenous injection: none was detected following 
      intraperitoneal or oral gavage administration of SkE. CONCLUSION: The LC-MS 
      methods were used for the quantitation of SkE in plant extracts and in mouse 
      blood. These methods open the way for further protocol optimisation of SkE 
      extraction and the determination of its pharmacokinetic data.
CI  - Copyright (c) 2014 John Wiley & Sons, Ltd.
FAU - Le, Hong Luyen
AU  - Le HL
AD  - Universite de Toulouse, UPS, UMR 152 Pharma-DEV, Universite Toulouse 3, Faculte 
      des Sciences Pharmaceutiques, F-31062, Toulouse cedex 09, France; Institut de 
      Recherche pour le Developpement (IRD), UMR 152 Pharma-DEV, F-31062, Toulouse 
      cedex 09, France.
FAU - Jullian, Valerie
AU  - Jullian V
FAU - Claparols, Catherine
AU  - Claparols C
FAU - Vansteelandt, Marieke
AU  - Vansteelandt M
FAU - Haddad, Mohamed
AU  - Haddad M
FAU - Cabou, Cendrine
AU  - Cabou C
FAU - Deharo, Eric
AU  - Deharo E
FAU - Fabre, Nicolas
AU  - Fabre N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20141127
PL  - England
TA  - Phytochem Anal
JT  - Phytochemical analysis : PCA
JID - 9200492
RN  - 0 (Plant Extracts)
RN  - 0 (Quassins)
RN  - 0 (simalikalactone E)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Male
MH  - Mice
MH  - Plant Extracts/chemistry/*isolation & purification
MH  - Plant Leaves/*chemistry
MH  - Plants, Medicinal
MH  - Quassia/*chemistry
MH  - Quassins/blood/chemistry/*isolation & purification
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - LC-MS
OT  - Quassia amara
OT  - biological fluids
OT  - plant extract
OT  - quantitation
OT  - quassinoids
OT  - simalikalactone E
EDAT- 2014/11/29 06:00
MHDA- 2016/01/08 06:00
CRDT- 2014/11/29 06:00
PHST- 2014/02/28 00:00 [received]
PHST- 2014/09/02 00:00 [revised]
PHST- 2014/09/04 00:00 [accepted]
PHST- 2014/11/29 06:00 [entrez]
PHST- 2014/11/29 06:00 [pubmed]
PHST- 2016/01/08 06:00 [medline]
AID - 10.1002/pca.2542 [doi]
PST - ppublish
SO  - Phytochem Anal. 2015 Mar-Apr;26(2):111-8. doi: 10.1002/pca.2542. Epub 2014 Nov 
      27.