PMID- 25433671
OWN - NLM
STAT- MEDLINE
DCOM- 20150928
LR  - 20181113
IS  - 1471-2229 (Electronic)
IS  - 1471-2229 (Linking)
VI  - 14
DP  - 2014 Nov 30
TI  - Analysis of the transcriptional responses in inflorescence buds of Jatropha 
      curcas exposed to cytokinin treatment.
PG  - 318
LID - 10.1186/s12870-014-0318-z [doi]
LID - 318
AB  - BACKGROUND: Jatropha curcas L. is a potential biofuel plant. Application of 
      exogenous cytokinin (6-benzyladenine, BA) on its inflorescence buds can 
      significantly increase the number of female flowers, thereby improving seed 
      yield. To investigate which genes and signal pathways are involved in the 
      response to cytokinin in J. curcas inflorescence buds, we monitored 
      transcriptional activity in inflorescences at 0, 3, 12, 24, and 48 h after BA 
      treatment using a microarray. RESULTS: We detected 5,555 differentially expressed 
      transcripts over the course of the experiment, which could be grouped into 12 
      distinct temporal expression patterns. We also identified 31 and 131 transcripts 
      in J. curcas whose homologs in model plants function in flowering and 
      phytohormonal signaling pathways, respectively. According to the transcriptional 
      analysis of genes involved in flower development, we hypothesized that BA 
      treatment delays floral organ formation by inhibiting the transcription of the A, 
      B and E classes of floral organ-identity genes, which would allow more time to 
      generate more floral primordia in inflorescence meristems, thereby enhancing 
      inflorescence branching and significantly increasing flower number per 
      inflorescence. BA treatment might also play an important role in maintaining the 
      flowering signals by activating the transcription of GIGANTEA (GI) and 
      inactivating the transcription of CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) and 
      TERMINAL FLOWER 1b (TFL1b). In addition, exogenous cytokinin treatment could 
      regulate the expression of genes involved in the metabolism and signaling of 
      other phytohormones, indicating that cytokinin and other phytohormones jointly 
      regulate flower development in J. curcas inflorescence buds. CONCLUSIONS: Our 
      study provides a framework to better understand the molecular mechanisms 
      underlying changes in flowering traits in response to cytokinin treatment in J. 
      curcas inflorescence buds. The results provide valuable information related to 
      the mechanisms of cross-talk among multiple phytohormone signaling pathways in 
      woody plants.
FAU - Chen, Mao-Sheng
AU  - Chen MS
FAU - Pan, Bang-Zhen
AU  - Pan BZ
FAU - Wang, Gui-Juan
AU  - Wang GJ
FAU - Ni, Jun
AU  - Ni J
FAU - Niu, Longjian
AU  - Niu L
FAU - Xu, Zeng-Fu
AU  - Xu ZF
LA  - eng
SI  - GEO/GSE54366
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141130
PL  - England
TA  - BMC Plant Biol
JT  - BMC plant biology
JID - 100967807
RN  - 0 (Benzyl Compounds)
RN  - 0 (Plant Growth Regulators)
RN  - 0 (Plant Proteins)
RN  - 0 (Purines)
RN  - KXG6A989PS (benzylaminopurine)
RN  - P39Y9652YJ (Kinetin)
SB  - IM
MH  - Benzyl Compounds
MH  - Gene Expression Regulation, Developmental/drug effects
MH  - Gene Expression Regulation, Plant/*drug effects
MH  - Inflorescence/*drug effects/genetics/growth & development/metabolism
MH  - Jatropha/*drug effects/genetics/growth & development/metabolism
MH  - Kinetin/*genetics/metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - Plant Growth Regulators/*genetics/metabolism
MH  - Plant Proteins/*genetics/metabolism
MH  - Purines
PMC - PMC4272566
EDAT- 2014/12/01 06:00
MHDA- 2015/09/29 06:00
PMCR- 2014/11/30
CRDT- 2014/12/01 06:00
PHST- 2014/06/21 00:00 [received]
PHST- 2014/11/06 00:00 [accepted]
PHST- 2014/12/01 06:00 [entrez]
PHST- 2014/12/01 06:00 [pubmed]
PHST- 2015/09/29 06:00 [medline]
PHST- 2014/11/30 00:00 [pmc-release]
AID - s12870-014-0318-z [pii]
AID - 318 [pii]
AID - 10.1186/s12870-014-0318-z [doi]
PST - epublish
SO  - BMC Plant Biol. 2014 Nov 30;14:318. doi: 10.1186/s12870-014-0318-z.