PMID- 25434124
OWN - NLM
STAT- MEDLINE
DCOM- 20150901
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 32
IP  - 8
DP  - 2014 Aug
TI  - [Simultaneous determination of 18 beta-agonist residues in feed using QuEChERS 
      sample preparation and high performance liquid chromatography-tandem mass 
      spectrometry].
PG  - 867-73
AB  - A multi-residue method was developed for the simultaneous determination of 18 
      beta-agonist residues (clenbuterol, ractopamine, penbutolol, tulobuterol, etc) in 
      feed by using QuEChERS sample preparation and high performance liquid 
      chromatography-tandem mass spectrometry (HPLC-MS/MS). The feed samples were 
      dispersed by water, then the analytes were extracted with acetonitrile containing 
      4% (v/v) ammonia and cleaned up by QuEChERS method with 25 mg octadecylsilyl 
      (C18) and 50 mg primary secondary amine (PSA) adsorbents. The separation of 
      compounds was carried on an Agilent ZORBAX Eclipse XDB-C,8 column (50 mm x 4. 6 
      mm, 1. 8 mum) by a gradient elution using methanol-0. 1% (v/v) formic acid aqueous 
      solution as mobile phase. The analytes were detected by tandem mass spectrometry 
      under multiple reaction monitoring (MRM) mode with positive electrospray 
      ionization (ESI+) and quantified by the matrix-matched external standard method. 
      The results showed that the calibration curves of the 18 beta-agonists were linear 
      in the range of 5 - 200 mug/L with correlation coefficients of 0. 9912-0. 9995. 
      The average recoveries of the 18 analytes at three spiked levels of 0.05, 0.1 and 
      0. 5 mg/kg ranged from 78. 4% to 107. 1% with the relative standard deviations 
      (RSDs) of 3.5%-12.3%. The limit of quantification (LOQ, S/N>/=10) was 0. 05 mg/kg 
      for each analyte. The developed method is simple and sensitive, and can be 
      applied as a screen and confirmatory method for the analysis of beta-agonists in 
      feed.
FAU - Zheng, Ling
AU  - Zheng L
FAU - Wu, Yujie
AU  - Wu Y
FAU - Zhao, Yongfeng
AU  - Zhao Y
FAU - Li, Lihua
AU  - Li L
FAU - Ma, Yanjuan
AU  - Ma Y
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Adrenergic beta-Agonists)
RN  - 0 (Phenethylamines)
RN  - 57370OZ3P1 (ractopamine)
RN  - 591I9SU0F7 (tulobuterol)
RN  - 78W62V43DY (Penbutolol)
RN  - N8ONU3L3PG (Terbutaline)
RN  - XTZ6AXU7KN (Clenbuterol)
SB  - IM
MH  - Adrenergic beta-Agonists/*analysis
MH  - Animal Feed/*analysis
MH  - *Chromatography, High Pressure Liquid
MH  - Clenbuterol
MH  - Drug Residues/*analysis
MH  - Penbutolol
MH  - Phenethylamines
MH  - *Tandem Mass Spectrometry
MH  - Terbutaline/analogs & derivatives
EDAT- 2014/12/02 06:00
MHDA- 2015/09/02 06:00
CRDT- 2014/12/02 06:00
PHST- 2014/12/02 06:00 [entrez]
PHST- 2014/12/02 06:00 [pubmed]
PHST- 2015/09/02 06:00 [medline]
AID - 10.3724/sp.j.1123.2014.03029 [doi]
PST - ppublish
SO  - Se Pu. 2014 Aug;32(8):867-73. doi: 10.3724/sp.j.1123.2014.03029.