PMID- 25434962
OWN - NLM
STAT- MEDLINE
DCOM- 20150924
LR  - 20151119
IS  - 1744-8387 (Electronic)
IS  - 1478-9450 (Linking)
VI  - 12
IP  - 1
DP  - 2015 Feb
TI  - Screening and identification of potential predictive biomarkers for Down's 
      syndrome from second trimester maternal serum.
PG  - 97-107
LID - 10.1586/14789450.2015.979796 [doi]
AB  - OBJECTIVE: In this study, we aimed to search for noninvasive predictive 
      biomarkers for prenatal diagnosis of Down's syndrome (DS). METHODS: Maternal 
      serum samples from five DS-affected pregnant women and five DS-unaffected women 
      were analyzed by 2D gel electrophoresis and MALDI-TOF mass spectrometry to screen 
      for potential predictive biomarkers of DS. Then, differential levels of dGTPase, 
      beta2-glycoprotein I (beta2-GPI), complement factor H-related protein 1 precursor 
      (CFHR1) and kininogen 1 isoform 2 were further verified by western blotting tests 
      in another independent group. RESULTS: Statistical analysis results revealed 29 
      protein spots whose levels differed significantly in the DS-affected pregnancies 
      group. Of these, the eight most differentially expressed in DP were identified 
      successfully. Among these, levels of dGTPase, CFHR1 and kininogen 1 were elevated 
      significantly, whereas beta2-GPI was reduced in DP. DISCUSSION: These preliminarily 
      verified proteins might serve as potential predictive biomarkers for DS-affected 
      pregnancies.
FAU - Yao, Yuan
AU  - Yao Y
AD  - Department of Laboratory Medicine, Southwest Hospital, Third Military Medical 
      University, Chongqing 400038, China.
FAU - Jiang, Ying
AU  - Jiang Y
FAU - Han, Mei
AU  - Han M
FAU - Xia, Yu
AU  - Xia Y
FAU - He, Yuchen
AU  - He Y
FAU - Wang, Yonghu
AU  - Wang Y
FAU - Luo, Yang
AU  - Luo Y
FAU - Zhang, Bo
AU  - Zhang B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141129
PL  - England
TA  - Expert Rev Proteomics
JT  - Expert review of proteomics
JID - 101223548
RN  - 0 (Biomarkers)
RN  - 0 (CFHR1 protein, human)
RN  - 0 (Complement C3b Inactivator Proteins)
RN  - 0 (Kininogens)
RN  - 0 (beta 2-Glycoprotein I)
SB  - IM
MH  - Adult
MH  - Biomarkers/blood
MH  - Complement C3b Inactivator Proteins
MH  - Down Syndrome/*blood
MH  - Female
MH  - Humans
MH  - Kininogens/blood
MH  - *Maternal Serum Screening Tests
MH  - Pregnancy
MH  - Pregnancy Trimester, Second/*blood
MH  - beta 2-Glycoprotein I/blood
OTO - NOTNLM
OT  - Down's syndrome
OT  - biomarker
OT  - maternal serum
OT  - prenatal screening
OT  - proteomics
EDAT- 2014/12/02 06:00
MHDA- 2015/09/25 06:00
CRDT- 2014/12/02 06:00
PHST- 2014/12/02 06:00 [entrez]
PHST- 2014/12/02 06:00 [pubmed]
PHST- 2015/09/25 06:00 [medline]
AID - 10.1586/14789450.2015.979796 [doi]
PST - ppublish
SO  - Expert Rev Proteomics. 2015 Feb;12(1):97-107. doi: 10.1586/14789450.2015.979796. 
      Epub 2014 Nov 29.