PMID- 25435229 OWN - NLM STAT- MEDLINE DCOM- 20150715 LR - 20191210 IS - 1873-3573 (Electronic) IS - 0039-9140 (Linking) VI - 133 DP - 2015 Feb TI - Successive determination of urinary bilirubin and creatinine employing simultaneous injection effective mixing flow analysis. PG - 71-6 LID - S0039-9140(14)00532-3 [pii] LID - 10.1016/j.talanta.2014.05.065 [doi] AB - A novel four-channel simultaneous injection effective mixing flow analysis (SIEMA) system has been assembled for successive determination of bilirubin and creatinine in urinary samples. The chemical variables and physical parameters in the flow system were optimized for the enhancement of successive analytical performances. The interferences from urine matrices on the determination of bilirubin and creatinine were eliminated to dilute urine samples. The calibration graphs with the optimum conditions were achieved to be in 0.024-5.0 mg L(-1) for bilirubin and 2-100 mg L(-1) for creatinine. The relative standard deviations (RSDs) at 3 mg L(-1) of bilirubin and at 50 mg L(-1) of creatinine for 11 runs were 1.5 and 1.0%, respectively. The limits of detections (3sigma of blank) for bilirubin and creatinine were 7 microg L(-1) and 0.6 mg L(-1), respectively. The sample throughput for stepwise detection was 22 h(-1). The proposed method was applied to the successive determination of bilirubin and creatinine in urine samples. CI - Copyright (c) 2014 Elsevier B.V. All rights reserved. FAU - Ponhong, Kraingkrai AU - Ponhong K AD - Department of Applied Chemistry, Aichi Institute of Technology, 1247 Yachigusa, Yakusa-cho, Toyota 470-0392, Japan; Department of Chemistry, Faculty of Science and Center of Excellence for Innovation in Analytical Science and Technology, Chiang Mai University, Chiang Mai 50200, Thailand. FAU - Teshima, Norio AU - Teshima N AD - Department of Applied Chemistry, Aichi Institute of Technology, 1247 Yachigusa, Yakusa-cho, Toyota 470-0392, Japan. Electronic address: teshima@aitech.ac.jp. FAU - Grudpan, Kate AU - Grudpan K AD - Department of Chemistry, Faculty of Science and Center of Excellence for Innovation in Analytical Science and Technology, Chiang Mai University, Chiang Mai 50200, Thailand. FAU - Vichapong, Jitlada AU - Vichapong J AD - Department of Applied Chemistry, Aichi Institute of Technology, 1247 Yachigusa, Yakusa-cho, Toyota 470-0392, Japan; Department of Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 4002, Thailand. FAU - Motomizu, Shoji AU - Motomizu S AD - Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan. FAU - Sakai, Tadao AU - Sakai T AD - Department of Applied Chemistry, Aichi Institute of Technology, 1247 Yachigusa, Yakusa-cho, Toyota 470-0392, Japan. LA - eng PT - Evaluation Study PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140708 PL - Netherlands TA - Talanta JT - Talanta JID - 2984816R RN - AYI8EX34EU (Creatinine) RN - RFM9X3LJ49 (Bilirubin) SB - IM MH - Bilirubin/*urine MH - Calibration MH - Creatinine/*urine MH - Equipment Design MH - Flow Injection Analysis/instrumentation MH - Humans MH - Limit of Detection MH - Urinalysis/*instrumentation OTO - NOTNLM OT - Bilirubin OT - Creatinine OT - Simultaneous injection effective mixing flow analysis OT - Urinalysis EDAT- 2014/12/02 06:00 MHDA- 2015/07/16 06:00 CRDT- 2014/12/02 06:00 PHST- 2014/01/21 00:00 [received] PHST- 2014/05/11 00:00 [revised] PHST- 2014/05/26 00:00 [accepted] PHST- 2014/12/02 06:00 [entrez] PHST- 2014/12/02 06:00 [pubmed] PHST- 2015/07/16 06:00 [medline] AID - S0039-9140(14)00532-3 [pii] AID - 10.1016/j.talanta.2014.05.065 [doi] PST - ppublish SO - Talanta. 2015 Feb;133:71-6. doi: 10.1016/j.talanta.2014.05.065. Epub 2014 Jul 8.