PMID- 25435788
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141201
LR  - 20181113
IS  - 1303-2968 (Print)
IS  - 1303-2968 (Electronic)
IS  - 1303-2968 (Linking)
VI  - 13
IP  - 4
DP  - 2014 Dec
TI  - Calcineurin-NFAT Signaling and Neurotrophins Control Transformation of Myosin 
      Heavy Chain Isoforms in Rat Soleus Muscle in Response to Aerobic Treadmill 
      Training.
PG  - 934-44
AB  - This study elucidated the role of CaN-NFAT signaling and neurotrophins on the 
      transformation of myosin heavy chain isoforms in the rat soleus muscle fiber 
      following aerobic exercise training. To do so, we examined the content and 
      distribution of myosin heavy chain (MyHC) isoforms in the rat soleus muscle 
      fiber, the activity of CaN and expression of NFATc1 in these fibers, and changes 
      in the expression of nerve growth factor (NGF), brain-derived neurotrophic factor 
      (BDNF) and neutrophin-3 (NT-3) in the soleus and striatum following high-and 
      medium-intensity aerobic treadmill training. Specific pathogen-free 2 month old 
      male Sprague-Dawley (SD) rats were randomly divided into three groups: Control 
      group (Con, n = 8), moderate-intensity aerobic exercise group (M-Ex, n = 8) and 
      high-intensity aerobic exercise group (H-Ex, n = 8). We used ATPase staining to 
      identify the muscle fiber type I and II, SDS-PAGE to separate and analyze the 
      isoforms MyHCI, MyHCIIA, MyHCIIB and MyHCIIx, and performed western blots to 
      determine the expression of NFATc1, NGF, BDNF and NT-3. CaN activity was measured 
      using a colorimetric assay. In the soleus muscle, 8 weeks of moderate-intensity 
      exercise can induce transformation of MyHC IIA and MyHC IIB to MyHC IIX and MyHC 
      I (p < 0.01), while high-intensity treadmill exercise can induce transform MyHC 
      IIx to MyHC IIB, MyHC IIA and MyHC I (p < 0.01). In comparison to the control 
      group, CaN activity and NFATcl protein level were significantly increased in both 
      the M-Ex and H-Ex groups (p < 0.05, p < 0.01), with a more pronounced 
      upregulation in the M-Ex group (p < 0.05). Eight weeks of moderate- and 
      high-intensity aerobic exercise induced the expression of NGF, BDNF and NT-3 in 
      the soleus muscle and the striatum (p < 0.01), with the most significant increase 
      in the H-Ex group (p < 0.01). In the rat soleus muscle, (1) CaN-NFATcl signaling 
      contributes to the conversion of MyHC I isoform in response to moderate-intensity 
      exercise; (2) Neurotrophins NGF, BDNF and NT-3 might play a role in the 
      conversion of MyHC II isoform in response to high-intensity treadmill exercise. 
      Key pointsEight weeks of moderate-intensity treadmill training induces the 
      transformation MyHC IIA and MyHC IIB to MyHC IIX and MyHC I in the soleus 
      muscles, while high-intensity exercise leads to transformation of MyHC IIX to 
      MyHC IIA, MyHC IIB and MyHC I.MyHC I conversion in response to moderate-intensity 
      aerobic exercise is mediated by calcineurin-NFATcl signaling.Eight weeks of 
      moderate- and high-ntensity aerobic exercise induces the expression of NGF, BDNF 
      and NT-3 in expression noted in rats subjected to high-intensity training. NGF 
      and NT-3 expression in the striatum is lower than in the soleus muscle, while 
      BDNF levels are similar. Neurotrophins may be involved in mediating MyHC II 
      conversion in response to high-intensity aerobic exercise.
FAU - Liu, Wenfeng
AU  - Liu W
AD  - Hunan Provincial Key Laboratory of Physical Fitness and Sports Rehabilitation, 
      Hunan Normal University , Qingyuan, Guangdong, China ; The Key Laboratory of 
      Protein Chemistry and Developmental Biology of Ministry of Education and Hunan 
      Normal University , Qingyuan, Guangdong, China.
FAU - Chen, Gan
AU  - Chen G
AD  - Hunan Provincial Key Laboratory of Physical Fitness and Sports Rehabilitation, 
      Hunan Normal University , Qingyuan, Guangdong, China.
FAU - Li, Fanling
AU  - Li F
AD  - Hunan Provincial People's Hospital, Hunan Normal University , Qingyuan, 
      Guangdong, China.
FAU - Tang, Changfa
AU  - Tang C
AD  - Hunan Provincial Key Laboratory of Physical Fitness and Sports Rehabilitation, 
      Hunan Normal University , Qingyuan, Guangdong, China.
FAU - Yin, Dazhong
AU  - Yin D
AD  - The Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of 
      Education and Hunan Normal University , Qingyuan, Guangdong, China ; Qingyuan 
      People's Hospital of Jinan University , Qingyuan, Guangdong, China.
LA  - eng
PT  - Journal Article
DEP - 20141201
PL  - Turkey
TA  - J Sports Sci Med
JT  - Journal of sports science & medicine
JID - 101174629
PMC - PMC4234965
OTO - NOTNLM
OT  - Aerobic treadmill training
OT  - Calcineurin/Nuclear factor of activated T cells c1
OT  - Myosin heavy chain isoforms transformation
OT  - neurotrophins
OT  - the muscle fiber type
EDAT- 2014/12/02 06:00
MHDA- 2014/12/02 06:01
PMCR- 2014/12/01
CRDT- 2014/12/02 06:00
PHST- 2014/07/26 00:00 [received]
PHST- 2014/09/08 00:00 [accepted]
PHST- 2014/12/02 06:00 [entrez]
PHST- 2014/12/02 06:00 [pubmed]
PHST- 2014/12/02 06:01 [medline]
PHST- 2014/12/01 00:00 [pmc-release]
AID - jssm-13-934 [pii]
PST - epublish
SO  - J Sports Sci Med. 2014 Dec 1;13(4):934-44. eCollection 2014 Dec.