PMID- 25436429
OWN - NLM
STAT- MEDLINE
DCOM- 20150310
LR  - 20141224
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 456
IP  - 1
DP  - 2015 Jan 2
TI  - Trans-Golgi protein p230/golgin-245 is involved in phagophore formation.
PG  - 275-81
LID - S0006-291X(14)02102-0 [pii]
LID - 10.1016/j.bbrc.2014.11.071 [doi]
AB  - p230/golgin-245 is a trans-Golgi coiled-coil protein that is known to participate 
      in regulatory transport from the trans-Golgi network (TGN) to the cell surface. 
      We investigated the role of p230 and its interacting protein, microtubule actin 
      crosslinking protein 1 (MACF1), in amino acid starvation-induced membrane 
      transport. p230 or MACF1 knock-down (KD) cells failed to increase the autophagic 
      flow rate and the number of microtubule-associated protein 1 light chain 3 
      (LC3)-positive puncta under starvation conditions. Loss of p230 or MACF1 impaired 
      mAtg9 recruitment to peripheral phagophores from the TGN, which was observed in 
      the early step of autophagosome formation. Overexpression of the p230-binding 
      domain of MACF1 resulted in the inhibition of mAtg9 trafficking in starvation 
      conditions as in p230-KD or MACF1-KD cells. These results indicate that p230 and 
      MACF1 cooperatively play an important role in the formation of phagophore through 
      starvation-induced transport of mAtg9-containing membranes from the TGN. In 
      addition, p230 itself was detected in autophagosomes/autolysosome with p62 or LC3 
      during autophagosome biogenesis. Thus, p230 is an important molecule in 
      phagophore formation, although it remains unclear whether p230 has any role in 
      late steps of autophagy.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Sohda, Miwa
AU  - Sohda M
AD  - Division of Oral Biochemistry, Niigata University Graduate School of Medical and 
      Dental Sciences, Chuo-ku, Niigata 951-8514, Japan.
FAU - Misumi, Yoshio
AU  - Misumi Y
AD  - Department of Cell Biology, Fukuoka University School of Medicine, Jonan-ku, 
      Fukuoka 814-0180, Japan. Electronic address: misumi@fukuoka-u.ac.jp.
FAU - Ogata, Shigenori
AU  - Ogata S
AD  - Joint Laboratory for Frontier Medical Science, Fukuoka University School of 
      Medicine, Jonan-ku, Fukuoka 814-0180, Japan.
FAU - Sakisaka, Shotaro
AU  - Sakisaka S
AD  - Department of Internal Medicine, Fukuoka University School of Medicine, Jonan-ku, 
      Fukuoka 814-0180, Japan.
FAU - Hirose, Shinichi
AU  - Hirose S
AD  - Department of Pediatrics, Fukuoka University School of Medicine, Jonan-ku, 
      Fukuoka 814-0180, Japan.
FAU - Ikehara, Yukio
AU  - Ikehara Y
AD  - Fukuoka Tenjin Medical Rehabilitation Academy, Chuo-ku, Fukuoka 810-0001, Japan.
FAU - Oda, Kimimitsu
AU  - Oda K
AD  - Division of Oral Biochemistry, Niigata University Graduate School of Medical and 
      Dental Sciences, Chuo-ku, Niigata 951-8514, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141128
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Actins)
RN  - 0 (Autoantigens)
RN  - 0 (GOLGA4 protein, human)
RN  - 0 (MACF1 protein, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Actins/metabolism
MH  - Autoantigens/*metabolism
MH  - *Autophagy
MH  - Biological Transport
MH  - Cell Membrane/metabolism
MH  - Golgi Apparatus/metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Membrane Proteins/*metabolism
MH  - Microfilament Proteins/*metabolism
MH  - Microtubules/metabolism
MH  - Phagosomes/metabolism
MH  - Plasmids/metabolism
MH  - Protein Transport
MH  - RNA, Small Interfering/metabolism
OTO - NOTNLM
OT  - Autophagy
OT  - Golgi
OT  - Immunochemistry
OT  - Membrane trafficking
OT  - siRNA
EDAT- 2014/12/02 06:00
MHDA- 2015/03/11 06:00
CRDT- 2014/12/02 06:00
PHST- 2014/11/07 00:00 [received]
PHST- 2014/11/19 00:00 [accepted]
PHST- 2014/12/02 06:00 [entrez]
PHST- 2014/12/02 06:00 [pubmed]
PHST- 2015/03/11 06:00 [medline]
AID - S0006-291X(14)02102-0 [pii]
AID - 10.1016/j.bbrc.2014.11.071 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2015 Jan 2;456(1):275-81. doi: 
      10.1016/j.bbrc.2014.11.071. Epub 2014 Nov 28.