PMID- 25437179
OWN - NLM
STAT- MEDLINE
DCOM- 20151130
LR  - 20181202
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Print)
IS  - 1547-3287 (Linking)
VI  - 24
IP  - 5
DP  - 2015 Mar 1
TI  - Umbilical cord mesenchymal stromal cells affected by gestational diabetes 
      mellitus display premature aging and mitochondrial dysfunction.
PG  - 575-86
LID - 10.1089/scd.2014.0349 [doi]
AB  - Human umbilical cord mesenchymal stromal cells (hUC-MSCs) of Wharton's jelly 
      origin undergo adipogenic, osteogenic, and chondrogenic differentiation in vitro. 
      Recent studies have consistently shown their therapeutic potential in various 
      human disease models. However, the biological effects of major pregnancy 
      complications on the cellular properties of hUC-MSCs remain to be studied. In 
      this study, we compared the basic properties of hUC-MSCs obtained from 
      gestational diabetes mellitus (GDM) patients (GDM-UC-MSCs) and normal pregnant 
      women (N-UC-MSCs). Assessments of cumulative cell growth, MSC marker expression, 
      cellular senescence, and mitochondrial function-related gene expression were 
      performed using a cell count assay, senescence-associated beta-galactosidase 
      staining, quantitative real-time reverse transcription-polymerase chain reaction, 
      immunoblotting, and cell-based mitochondrial functional assay system. When 
      compared with N-UC-MSCs, GDM-UC-MSCs showed decreased cell growth and earlier 
      cellular senescence with accumulation of p16 and p53, even though they expressed 
      similar levels of CD105, CD90, and CD73 MSC marker proteins. GDM-UC-MSCs also 
      displayed significantly lower osteogenic and adipogenic differentiation 
      potentials than N-UC-MSCs. Furthermore, GDM-UC-MSCs exhibited a low mitochondrial 
      activity and significantly reduced expression of the mitochondrial function 
      regulatory genes ND2, ND9, COX1, PGC-1alpha, and TFAM. Here, we report intriguing and 
      novel evidence that maternal metabolic derangement during gestation affects the 
      biological properties of fetal cells, which may be a component of fetal 
      programming. Our findings also underscore the importance of the critical 
      assessment of the biological impact of maternal-fetal conditions in biological 
      studies and clinical applications of hUC-MSCs.
FAU - Kim, Jooyeon
AU  - Kim J
AD  - 1 Departments of Pathology, Asan Medical Center, University of Ulsan College of 
      Medicine , Seoul, Korea.
FAU - Piao, Ying
AU  - Piao Y
FAU - Pak, Youngmi Kim
AU  - Pak YK
FAU - Chung, Dalhee
AU  - Chung D
FAU - Han, Yu Mi
AU  - Han YM
FAU - Hong, Joon Seok
AU  - Hong JS
FAU - Jun, Eun Jeong
AU  - Jun EJ
FAU - Shim, Jae-Yoon
AU  - Shim JY
FAU - Choi, Jene
AU  - Choi J
FAU - Kim, Chong Jai
AU  - Kim CJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150114
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
SB  - IM
EIN - Stem Cells Dev. 2015 May 15;24(10):1263. PMID: 25928834
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cellular Senescence
MH  - Diabetes, Gestational/metabolism/*pathology
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Mesenchymal Stem Cells/*physiology
MH  - Mitochondria/*metabolism
MH  - Oxidative Phosphorylation
MH  - Pregnancy
MH  - Umbilical Cord/pathology
PMC - PMC4333320
EDAT- 2014/12/02 06:00
MHDA- 2015/12/15 06:00
PMCR- 2016/03/01
CRDT- 2014/12/02 06:00
PHST- 2014/12/02 06:00 [entrez]
PHST- 2014/12/02 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
PHST- 2016/03/01 00:00 [pmc-release]
AID - 10.1089/scd.2014.0349 [pii]
AID - 10.1089/scd.2014.0349 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2015 Mar 1;24(5):575-86. doi: 10.1089/scd.2014.0349. Epub 2015 
      Jan 14.