PMID- 25438100
OWN - NLM
STAT- MEDLINE
DCOM- 20151023
LR  - 20141220
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 60
IP  - 2
DP  - 2015 Feb
TI  - Fluoride alters connexin expression in rat incisor pulp.
PG  - 313-9
LID - S0003-9969(14)00283-0 [pii]
LID - 10.1016/j.archoralbio.2014.11.003 [doi]
AB  - OBJECTIVE: Connexins (Cxs) are important to control growth and cell 
      differentiation of dental tissues. The aim of the present study was to assess the 
      impact of chronic exposure to sodium fluoride (NaF) on Cxs expression and 
      alkaline phosphatase (ALP) activity in dental pulp, and on morphometric 
      parameters of adult rat mandible and incisors. DESIGN: Three groups of male 
      Wistar rats (22 days-old) were given water containing: (a) 0.3 mg/L (Control), 
      (b) 10 mg/L and (c) 50 mg/L of NaF for eight weeks. Incisor pulp homogenates were 
      prepared for determination of Cx32, Cx43 and Cx45 gene expression, using 
      semi-quantitative RT-PCR, and of ALP activity. Morphometric parameters of 
      mandible and incisors were determined on radiographs. RESULTS: Cx43 gene 
      expression increased with exposure to NaF in a dose-dependent manner. Cx32 mRNA 
      levels were higher than controls in the 10mg/L NaF group only; Cx45 mRNA levels 
      were lower in groups given 10 and 50mg/L of NaF than in controls. ALP activity 
      was higher in both high-NaF dose groups compared to the control group (p<0.05). 
      Lower incisor diameter was lower in the 50 mg/L NaF than in the control group 
      (p<0.01). None of the mandibular growth parameters were affected by NaF 
      treatment. CONCLUSION: Our results showed that fluorotic alterations in rat 
      incisor were associated with increased Cx43 expression and ALP activity, as well 
      as with changes in the expression pattern of different Cxs in pulp tissue. The 
      observed changes may have a stimulating effect on dentin mineralization.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Centeno, V A
AU  - Centeno VA
AD  - Department of Biological Chemistry, School of Dentistry, National University of 
      Cordoba, Cordoba, Argentina. Electronic address: vcenteno@odo.unc.edu.ar.
FAU - Fontanetti, P A
AU  - Fontanetti PA
AD  - Department of Biological Chemistry, School of Dentistry, National University of 
      Cordoba, Cordoba, Argentina.
FAU - Interlandi, V
AU  - Interlandi V
AD  - Department of Biological Chemistry, School of Dentistry, National University of 
      Cordoba, Cordoba, Argentina.
FAU - Ponce, R H
AU  - Ponce RH
AD  - Department of Biological Chemistry, School of Dentistry, National University of 
      Cordoba, Cordoba, Argentina.
FAU - Gallara, R V
AU  - Gallara RV
AD  - Department of Biological Chemistry, School of Dentistry, National University of 
      Cordoba, Cordoba, Argentina.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141111
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Connexins)
RN  - 8ZYQ1474W7 (Sodium Fluoride)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Connexins/genetics/*metabolism
MH  - Dental Pulp/*drug effects/*metabolism
MH  - Gene Expression
MH  - Incisor
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Real-Time Polymerase Chain Reaction
MH  - Sodium Fluoride/*pharmacology
OTO - NOTNLM
OT  - Connexins
OT  - Dental pulp tissue
OT  - Rat dental fluorosis
OT  - Sodium fluoride
EDAT- 2014/12/02 06:00
MHDA- 2015/10/24 06:00
CRDT- 2014/12/02 06:00
PHST- 2014/06/04 00:00 [received]
PHST- 2014/09/18 00:00 [revised]
PHST- 2014/11/04 00:00 [accepted]
PHST- 2014/12/02 06:00 [entrez]
PHST- 2014/12/02 06:00 [pubmed]
PHST- 2015/10/24 06:00 [medline]
AID - S0003-9969(14)00283-0 [pii]
AID - 10.1016/j.archoralbio.2014.11.003 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2015 Feb;60(2):313-9. doi: 10.1016/j.archoralbio.2014.11.003. 
      Epub 2014 Nov 11.