PMID- 25438246
OWN - NLM
STAT- MEDLINE
DCOM- 20150803
LR  - 20141216
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 975
DP  - 2015 Jan 15
TI  - Selective adsorption of antiphospholipid and anti-dsDNA autoantibodies on 
      histidine based pseudobioaffinity adsorbent from sera of patients with systemic 
      lupus erythematosus (SLE).
PG  - 77-83
LID - S1570-0232(14)00694-1 [pii]
LID - 10.1016/j.jchromb.2014.11.005 [doi]
AB  - Systemic lupus erythematosus (SLE) is an autoimmune diseases characterized by the 
      presence of antiphospholipid and anti-dsDNA autoantibodies in the sera of 
      patients. These autoantibodies and their subclasses have received increasing 
      attention by medical community due to their association with recurrent venous 
      thrombosis, fetal loss and thrombocytopenia. In particular, attention has been 
      paid to IgG subclasses in SLE. The biological and functional properties together 
      with the subclass distribution might therefore influence the course of SLE. The 
      separation and elimination of these autoantibodies from sera of patients can be 
      effective in clinical therapy. In the present study, histidine based 
      pseudobioaffinity adsorbents have been used for the selective adsorption and 
      separation of anti-double stranded DNA (anti-dsDNA), anticardiolipin (aCL) and 
      anti-beta2-glycoprotein-I (anti-beta2-GPI) antibodies from sera of patients with SLE. 
      For this purpose histidine acting as a pseudobiospecific ligand has been coupled 
      to bisoxirane activated sepharose CL-6B for the adsorption and separation of 
      these autoantibodies. The removal of autoantibodies was carried out under gentle 
      adsorption and elution chromatographic conditions at pH values 7.0 and 8.0. 
      Autoantibodies isotypes and subclasses distribution in the separated fractions 
      were studied by enzyme-linked immune-sorbent assay. The obtained results showed 
      that the separated anticardiolipin and anti-beta2-glycoprotein-I autoantibodies 
      belong to IgG1, IgG2 and IgG3subclasses, while those of anti-dsDNA belong to IgM 
      isotype and were shown to have a DNA hydrolyzing activity that hydrolyzes plasmid 
      DNA. The results also indicate a total IgM and IgG recovery superior to 90% of 
      the fraction loaded at pH 7.4 and pH 8.0 respectively.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Zeinab, Mcheik
AU  - Zeinab M
AD  - Laboratoire de Valorisation des Ressources Naturelles et Produits de Sante 
      (VRNPS), EDST, Lebanese University, Rafic Hariri University Campus, Hadath, 
      Lebanon.
FAU - Hiam, Khoury-Matta
AU  - Hiam KM
AD  - El Arz Hospital, Department of Rheumatology, Zalka, Metn, Lebanon.
FAU - Edmond, Cheble
AU  - Edmond C
AD  - Department of Chemistry, Faculty of Pharmacy, Lebanese University, Rafic Hariri 
      University Campus, Hadath, Lebanon.
FAU - Assem, Elkak
AU  - Assem E
AD  - Laboratoire de Valorisation des Ressources Naturelles et Produits de Sante 
      (VRNPS), EDST, Lebanese University, Rafic Hariri University Campus, Hadath, 
      Lebanon. Electronic address: elkak@hotmail.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141115
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Autoantibodies)
RN  - 0 (Epoxy Compounds)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (bisoxirane)
RN  - 4QD397987E (Histidine)
RN  - 9007-49-2 (DNA)
RN  - 9012-36-6 (Sepharose)
SB  - IM
MH  - Adsorption
MH  - Adult
MH  - Aged
MH  - Autoantibodies/blood/*immunology/*isolation & purification
MH  - Chromatography, Liquid/*methods
MH  - DNA/*immunology
MH  - Epoxy Compounds/chemistry
MH  - Female
MH  - Histidine/chemistry
MH  - Humans
MH  - Immunoglobulin G/blood/immunology/isolation & purification
MH  - Immunoglobulin M/blood/immunology/isolation & purification
MH  - Lupus Erythematosus, Systemic/*blood/*immunology
MH  - Male
MH  - Middle Aged
MH  - Sepharose/chemistry
MH  - Young Adult
OTO - NOTNLM
OT  - Autoantibodies
OT  - Autoantibodies subclasses
OT  - DNA hydrolysis
OT  - Histidine
OT  - Pseudobioaffinity chromatography
OT  - SLE
EDAT- 2014/12/02 06:00
MHDA- 2015/08/04 06:00
CRDT- 2014/12/02 06:00
PHST- 2014/08/05 00:00 [received]
PHST- 2014/10/29 00:00 [revised]
PHST- 2014/11/08 00:00 [accepted]
PHST- 2014/12/02 06:00 [entrez]
PHST- 2014/12/02 06:00 [pubmed]
PHST- 2015/08/04 06:00 [medline]
AID - S1570-0232(14)00694-1 [pii]
AID - 10.1016/j.jchromb.2014.11.005 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Jan 15;975:77-83. doi: 
      10.1016/j.jchromb.2014.11.005. Epub 2014 Nov 15.