PMID- 25442284
OWN - NLM
STAT- MEDLINE
DCOM- 20150330
LR  - 20220317
IS  - 1618-095X (Electronic)
IS  - 0944-7113 (Linking)
VI  - 21
IP  - 12
DP  - 2014 Oct 15
TI  - Oroxylin A, a constituent of Oroxylum indicum inhibits adipogenesis and induces 
      apoptosis in 3T3-L1 cells.
PG  - 1733-41
LID - S0944-7113(14)00329-8 [pii]
LID - 10.1016/j.phymed.2014.08.014 [doi]
AB  - Oroxylin A (OA) is a flavonoid found in Oroxylum indicum, a medicinal plant with 
      multiple biological activities. This study was taken up to investigate the effect 
      of OA, on adipogenesis, lipolysis and apoptosis in 3T3 L1 cells. Pre-adipocytes 
      were treated with 10-40 muM OA on various days of adipogenesis treatment schedule. 
      Mature adipocytes were treated with OA for lipolysis and apoptosis studies. In 
      maturing pre-adipocytes, 10 muM OA suppressed intracellular lipid accumulation by 
      42.19% which was confirmed by lipidTox imaging of cells. In addition, OA 
      decreased the nuclear translocation of PPARgamma and mRNA expression of its 
      downstream genes (FAS and LPL) along with adiponectin secretion. In mature 
      adipocytes, 40 muM of OA decreased cell viability by 30% of control. Annexin V/PI 
      staining showed induction of apoptosis which was further confirmed by enhanced 
      levels of pro-apoptotic proteins Bax, cyt c, AIF and chromatin condensation. OA 
      enhanced TNF-alpha secretion, lipolysis and decreased Akt phosphorylation in mature 
      adipocytes. Findings suggest that OA possibly exerts its anti-obesity effect by 
      affecting adipocyte life cycle at critical points of differentiation and 
      maturity. When we compared the potency of OA with non-methoxylated flavonoids 
      morin, naringenin and kaempferol on adipocyte life cycle OA was far more potent. 
      Thus, study clearly indicates a new role for oroxylin A as regulator of adipocyte 
      life cycle. In addition, study also suggested a specific role of methoxylated 
      group in exerting lipolysis and cytotoxic effects in mature adipocytes.
CI  - Copyright (c) 2014 Elsevier GmbH. All rights reserved.
FAU - Singh, Jyotsna
AU  - Singh J
AD  - Herbal Research Section, CSIR-Indian Institute of Toxicology Research, Post Box 
      No. 80, Mahatma Gandhi Marg, Lucknow 226001, India.
FAU - Kakkar, Poonam
AU  - Kakkar P
AD  - Herbal Research Section, CSIR-Indian Institute of Toxicology Research, Post Box 
      No. 80, Mahatma Gandhi Marg, Lucknow 226001, India. Electronic address: 
      pkakkar@iitr.res.in.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140917
PL  - Germany
TA  - Phytomedicine
JT  - Phytomedicine : international journal of phytotherapy and phytopharmacology
JID - 9438794
RN  - 0 (Flavonoids)
RN  - 53K24Z586G (5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/*drug effects
MH  - Adipogenesis/*drug effects
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Bignoniaceae/chemistry
MH  - Flavonoids/*pharmacology
MH  - Lipolysis/drug effects
MH  - Mice
MH  - Plants, Medicinal/chemistry
OTO - NOTNLM
OT  - Adipogenesis
OT  - Apoptosis
OT  - Lipolysis
OT  - Oroxylin A
OT  - Oroxylum indicum
OT  - PPARgamma
EDAT- 2014/12/03 06:00
MHDA- 2015/03/31 06:00
CRDT- 2014/12/03 06:00
PHST- 2014/02/14 00:00 [received]
PHST- 2014/07/30 00:00 [revised]
PHST- 2014/08/24 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2015/03/31 06:00 [medline]
AID - S0944-7113(14)00329-8 [pii]
AID - 10.1016/j.phymed.2014.08.014 [doi]
PST - ppublish
SO  - Phytomedicine. 2014 Oct 15;21(12):1733-41. doi: 10.1016/j.phymed.2014.08.014. 
      Epub 2014 Sep 17.