PMID- 25444360
OWN - NLM
STAT- MEDLINE
DCOM- 20170327
LR  - 20191210
IS  - 1578-1852 (Electronic)
IS  - 0213-005X (Linking)
VI  - 33
IP  - 6
DP  - 2015 Jun-Jul
TI  - [Evaluation of mass spectrometry for the identification of clinically interesting 
      yeasts].
PG  - 372-8
LID - S0213-005X(14)00340-1 [pii]
LID - 10.1016/j.eimc.2014.10.003 [doi]
AB  - INTRODUCTION: Identification of yeasts is based on morphological, biochemical and 
      nutritional characteristics, and using molecular methods. Matrix-assisted laser 
      desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, a new method 
      for the identification of microorganisms, has demonstrated to be very useful. The 
      aim of this study is to evaluate this new method in the identification of yeasts. 
      METHODS: A total of 600 strains of yeasts isolated from clinical specimens 
      belonging to 9 genera and 43 species were tested. Identification was made by 
      sequencing of the ITS regions of ribosomal DNA, assimilation of carbon compounds 
      (ID 32C), and mass spectrometry on a Microflex spectrometer (Bruker Daltonics 
      GmbH, Germany). RESULTS: A total of 569 strains (94.8%) were identified to 
      species level by ID 32C, and 580 (96.7%) by MALDI-TOF. Concordance between both 
      methods was observed for 553 strains (92.2%), with 100% in clinically relevant 
      species: C. albicans, C. glabrata, C. parapsilosis, C. tropicalis, and almost 
      100% in C. krusei. MALDI-TOF identified species requiring molecular methods: 
      Candida dubliniensis, C. nivariensis, C. metapsilosis and C. orthopsilosis. Some 
      irregularities were observed in the identification of arthroconidia yeast and 
      basidiomycetes. CONCLUSION: MALDI-TOF is a rapid, effective and economic method, 
      which enables the identification of most clinically important yeasts and the 
      differentiation of closely related species. It would be desirable to include more 
      species in its database to expand its performance.
CI  - Copyright (c) 2014 Elsevier Espana, S.L.U. y Sociedad Espanola de Enfermedades 
      Infecciosas y Microbiologia Clinica. All rights reserved.
FAU - Galan, Fatima
AU  - Galan F
AD  - Servicio de Microbiologia, Hospital Puerta del Mar, Cadiz, Espana.
FAU - Garcia-Agudo, Lidia
AU  - Garcia-Agudo L
AD  - Servicio de Microbiologia, Hospital Virgen de la Salud, Toledo, Espana.
FAU - Guerrero, Inmaculada
AU  - Guerrero I
AD  - Servicio de Microbiologia, Hospital Puerta del Mar, Cadiz, Espana.
FAU - Marin, Pilar
AU  - Marin P
AD  - Servicio de Microbiologia, Hospital Puerta del Mar, Cadiz, Espana.
FAU - Garcia-Tapia, Ana
AU  - Garcia-Tapia A
AD  - Servicio de Microbiologia, Hospital Puerta del Mar, Cadiz, Espana.
FAU - Garcia-Martos, Pedro
AU  - Garcia-Martos P
AD  - Servicio de Microbiologia, Hospital Puerta del Mar, Cadiz, Espana. Electronic 
      address: pedromartos@hotmail.com.
FAU - Rodriguez-Iglesias, Manuel
AU  - Rodriguez-Iglesias M
AD  - Servicio de Microbiologia, Hospital Puerta del Mar, Cadiz, Espana.
LA  - spa
PT  - Evaluation Study
PT  - Journal Article
TT  - Evaluacion de la espectrometria de masas en la identificacion de levaduras de 
      interes clinico.
DEP - 20141129
PL  - Spain
TA  - Enferm Infecc Microbiol Clin
JT  - Enfermedades infecciosas y microbiologia clinica
JID - 9104081
RN  - 0 (Carbon Isotopes)
RN  - 0 (DNA, Fungal)
RN  - 0 (DNA, Ribosomal Spacer)
SB  - IM
MH  - Candida/classification/isolation & purification
MH  - Carbon Isotopes/metabolism
MH  - DNA, Fungal/analysis
MH  - DNA, Ribosomal Spacer
MH  - Humans
MH  - Mycological Typing Techniques/*methods
MH  - Mycoses/*microbiology
MH  - Reproducibility of Results
MH  - Species Specificity
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
MH  - Yeasts/classification/*isolation & purification/metabolism
OTO - NOTNLM
OT  - Candida
OT  - Espectrometria de masas MALDI-TOF
OT  - Levaduras
OT  - Levaduras de interes clinico
OT  - MALDI-TOF mass spectrometry
OT  - Medically-important yeasts
OT  - Yeasts
EDAT- 2014/12/03 06:00
MHDA- 2017/03/28 06:00
CRDT- 2014/12/03 06:00
PHST- 2014/03/11 00:00 [received]
PHST- 2014/07/29 00:00 [revised]
PHST- 2014/10/01 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2017/03/28 06:00 [medline]
AID - S0213-005X(14)00340-1 [pii]
AID - 10.1016/j.eimc.2014.10.003 [doi]
PST - ppublish
SO  - Enferm Infecc Microbiol Clin. 2015 Jun-Jul;33(6):372-8. doi: 
      10.1016/j.eimc.2014.10.003. Epub 2014 Nov 29.