PMID- 25445346 OWN - NLM STAT- MEDLINE DCOM- 20150916 LR - 20150113 IS - 1872-7492 (Electronic) IS - 0168-1702 (Linking) VI - 196 DP - 2015 Jan 22 TI - Transcription analysis on response of porcine alveolar macrophages to co-infection of the highly pathogenic porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae. PG - 60-9 LID - S0168-1702(14)00450-X [pii] LID - 10.1016/j.virusres.2014.11.006 [doi] AB - Porcine respiratory disease complex (PRDC) is of great concern economically, for swine producers worldwide. Co-infections with porcine reproductive and respiratory syndrome virus (PRRSV) and Mycoplasma hyopneumoniae (Mhp) are considered the major causative agents of PRDC, and responsible for mass mortality in pigs. Nevertheless, the molecular mechanisms underlying the host factors involved in pathogenesis and persistent infection have not been clearly established because of a lack of information regarding host responses following co-infection. In the current study, high throughput cDNA microarray assays were employed to evaluate host responses of porcine alveolar macrophages (PAM) to co-infection with highly pathogenic PRRSV (HP-PRRSV) and Mhp. A total of 2152 and 1760 genes were identified as being differentially expressed between the control group and PRRSV+Mhp co-infected group at 6 and 15 h post infection, respectively. The DE genes were involved in many vital functional classes, including inflammatory response, immune response, apoptosis, defense response, signal transduction. The pathway analysis demonstrated that the most significant pathways were associated with chemokine signaling pathway, cytokine, TLR, RLR and NLR signaling pathways and Jak-STAT signaling pathway. STRING analysis demonstrated that IL-1beta is an integral gene in co-infections with PRRSV and Mhp. The present study is the first to document the response of PAMs to co-infection with HP-PRRSV and Mhp. The observed gene expression profile could help with the screening of potential host agents for reducing the prevalence of co-infections, and to further develop our understanding of the molecular pathogenesis associated with PRRSV and Mhp co-infection in pigs. CI - Copyright (c) 2014 Elsevier B.V. All rights reserved. FAU - Li, Bin AU - Li B AD - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, Jiangsu Province, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. FAU - Du, Luping AU - Du L AD - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, Jiangsu Province, China. FAU - Xu, Xiangwei AU - Xu X AD - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, Jiangsu Province, China. FAU - Sun, Bing AU - Sun B AD - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, Jiangsu Province, China. FAU - Yu, Zhengyu AU - Yu Z AD - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, Jiangsu Province, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. FAU - Feng, Zhixin AU - Feng Z AD - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, Jiangsu Province, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. FAU - Liu, Maojun AU - Liu M AD - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, Jiangsu Province, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. FAU - Wei, Yanna AU - Wei Y AD - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, Jiangsu Province, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. FAU - Wang, Haiyan AU - Wang H AD - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, Jiangsu Province, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. FAU - Shao, Guoqing AU - Shao G AD - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, Jiangsu Province, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. Electronic address: 84391973@163.com. FAU - He, Kongwang AU - He K AD - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, Jiangsu Province, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. Electronic address: kwh2003@263.net. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20141113 PL - Netherlands TA - Virus Res JT - Virus research JID - 8410979 RN - 0 (Interleukin-1beta) SB - IM MH - Animals MH - Cluster Analysis MH - Coinfection MH - Computational Biology MH - Gene Expression Profiling MH - Host-Pathogen Interactions/genetics MH - Interleukin-1beta/genetics/metabolism MH - Macrophages, Alveolar/immunology/*metabolism MH - Molecular Sequence Annotation MH - *Mycoplasma hyopneumoniae MH - Pneumonia of Swine, Mycoplasmal/*genetics/immunology/metabolism MH - Porcine Reproductive and Respiratory Syndrome/*genetics/immunology/metabolism MH - *Porcine respiratory and reproductive syndrome virus MH - Reproducibility of Results MH - Signal Transduction MH - Swine MH - *Transcriptome OTO - NOTNLM OT - Co-infection OT - Microarray analysis OT - Mycoplasma hyopneumoniae OT - PRRSV EDAT- 2014/12/03 06:00 MHDA- 2015/09/17 06:00 CRDT- 2014/12/03 06:00 PHST- 2014/09/02 00:00 [received] PHST- 2014/11/05 00:00 [revised] PHST- 2014/11/06 00:00 [accepted] PHST- 2014/12/03 06:00 [entrez] PHST- 2014/12/03 06:00 [pubmed] PHST- 2015/09/17 06:00 [medline] AID - S0168-1702(14)00450-X [pii] AID - 10.1016/j.virusres.2014.11.006 [doi] PST - ppublish SO - Virus Res. 2015 Jan 22;196:60-9. doi: 10.1016/j.virusres.2014.11.006. Epub 2014 Nov 13.