PMID- 25445909
OWN - NLM
STAT- MEDLINE
DCOM- 20150825
LR  - 20231213
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 49
IP  - 1
DP  - 2015 Mar
TI  - Characterization of surface phenotypic molecules of teleost dendritic cells.
PG  - 38-43
LID - S0145-305X(14)00274-2 [pii]
LID - 10.1016/j.dci.2014.11.010 [doi]
AB  - Dendritic cells (DCs) are among the most important professional 
      antigen-presenting cells (APCs) that participate in various biological activities 
      in mammals. However, evidence of the existence of DCs in teleost fish and other 
      lower vertebrates remains limited. In this study, phenotypic and functional 
      characteristics of teleost DCs were described in a zebrafish model. An improved 
      method to efficiently enrich DCs was established. Immunofluorescence staining 
      revealed that the surface phenotypic hallmarks of mammalian DCs, including 
      MHC-II, CD80/86, CD83, and CD209, were distributed on the surfaces of zebrafish 
      DCs (DrDCs). Functional analysis results showed that DrDCs could initiate 
      antigen-specific CD4(+) T cell activation, in which MHC-II, CD80/86, CD83, and 
      CD209 are implicated. Hence, teleost DCs exhibit conserved immunophenotypes and 
      functions similar to those of their mammalian counterparts. Our findings 
      contributed to the current understanding of the evolutionary history of DCs and 
      the DC-regulatory mechanisms of adaptive immunity.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Shao, Tong
AU  - Shao T
AD  - College of Life Sciences, Zhejiang University, Hangzhou 310058, China; Key 
      Laboratory for Cell and Gene Engineering of Zhejiang Province, Hangzhou 310058, 
      China.
FAU - Zhu, Lv-Yun
AU  - Zhu LY
AD  - College of Science, National University of Defense Technology, Changsha, Hunan 
      410073, China.
FAU - Nie, Li
AU  - Nie L
AD  - College of Life Sciences, Zhejiang University, Hangzhou 310058, China; Key 
      Laboratory for Cell and Gene Engineering of Zhejiang Province, Hangzhou 310058, 
      China.
FAU - Shi, Wei
AU  - Shi W
AD  - College of Life Sciences, Zhejiang University, Hangzhou 310058, China; Key 
      Laboratory for Cell and Gene Engineering of Zhejiang Province, Hangzhou 310058, 
      China.
FAU - Dong, Wei-Ren
AU  - Dong WR
AD  - College of Life Sciences, Zhejiang University, Hangzhou 310058, China; Key 
      Laboratory for Cell and Gene Engineering of Zhejiang Province, Hangzhou 310058, 
      China.
FAU - Xiang, Li-Xin
AU  - Xiang LX
AD  - College of Life Sciences, Zhejiang University, Hangzhou 310058, China; Key 
      Laboratory for Cell and Gene Engineering of Zhejiang Province, Hangzhou 310058, 
      China. Electronic address: xianglx@zju.edu.cn.
FAU - Shao, Jian-Zhong
AU  - Shao JZ
AD  - College of Life Sciences, Zhejiang University, Hangzhou 310058, China; Key 
      Laboratory for Cell and Gene Engineering of Zhejiang Province, Hangzhou 310058, 
      China. Electronic address: shaojz@zju.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141113
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (Antigens, CD)
RN  - 0 (B7-1 Antigen)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (GATA2 Transcription Factor)
RN  - 0 (Gata2a protein, zebrafish)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Immunoglobulins)
RN  - 0 (Interleukin-12 Subunit p40)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Zebrafish Proteins)
RN  - EC 2.7.10.1 (Receptor, Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Antigen-Presenting Cells/*immunology/metabolism
MH  - Antigens, CD/genetics/immunology/metabolism
MH  - B7-1 Antigen/genetics/immunology/metabolism
MH  - Cell Adhesion Molecules/genetics/immunology/metabolism
MH  - Cell Membrane/*immunology/metabolism
MH  - Dendritic Cells/*immunology/metabolism
MH  - Female
MH  - Flow Cytometry
MH  - GATA2 Transcription Factor/genetics/immunology/metabolism
MH  - Gene Expression/immunology
MH  - Histocompatibility Antigens Class II/genetics/immunology/metabolism
MH  - Immunoglobulins/genetics/immunology/metabolism
MH  - Immunophenotyping
MH  - Interleukin-12 Subunit p40/genetics/immunology/metabolism
MH  - Lectins, C-Type/genetics/immunology/metabolism
MH  - Lymphocyte Activation/immunology
MH  - Male
MH  - Membrane Glycoproteins/genetics/immunology/metabolism
MH  - Microscopy, Confocal
MH  - Receptor, Macrophage Colony-Stimulating Factor/genetics/immunology/metabolism
MH  - Receptors, Antigen, T-Cell/genetics/immunology/metabolism
MH  - Receptors, Cell Surface/genetics/immunology/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Zebrafish/genetics/*immunology/metabolism
MH  - Zebrafish Proteins/genetics/immunology/metabolism
MH  - CD83 Antigen
OTO - NOTNLM
OT  - Antigen presentation
OT  - Co-stimulatory signals
OT  - Surface phenotypic molecules
OT  - Zebrafish dendritic cells
EDAT- 2014/12/03 06:00
MHDA- 2015/08/26 06:00
CRDT- 2014/12/03 06:00
PHST- 2014/10/10 00:00 [received]
PHST- 2014/11/09 00:00 [revised]
PHST- 2014/11/10 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2015/08/26 06:00 [medline]
AID - S0145-305X(14)00274-2 [pii]
AID - 10.1016/j.dci.2014.11.010 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2015 Mar;49(1):38-43. doi: 10.1016/j.dci.2014.11.010. Epub 2014 
      Nov 13.