PMID- 25446127
OWN - NLM
STAT- MEDLINE
DCOM- 20150310
LR  - 20161125
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 456
IP  - 1
DP  - 2015 Jan 2
TI  - Fibroblast growth factor 10 protects neuron against oxygen-glucose deprivation 
      injury through inducing heme oxygenase-1.
PG  - 225-31
LID - S0006-291X(14)02093-2 [pii]
LID - 10.1016/j.bbrc.2014.11.063 [doi]
AB  - Fibroblast growth factors (FGFs) are a family of structurally related 
      heparin-binding proteins with diverse biological functions. FGFs participate in 
      mitogenesis, angiogenesis, cell proliferation, development, differentiation and 
      cell migration. Here, we investigated the potential effect of FGF10, a member of 
      FGFs, on neuron survival in oxygen-glucose deprivation (OGD) model. In primary 
      cultured mouse cortical neurons upon OGD, FGF10 treatment (100 and 1000 ng/ml) 
      attenuated the decrease of cell viability and rescued the LDH release. Tuj-1 
      immunocytochemistry assay showed that FGF10 promoted neuronal survival. Apoptosis 
      assay with Annexin V+PI by flow cytometry demonstrated that FGF10 treatment 
      reduced apoptotic cell proportion. Moreover, immunoblotting showed that FGF10 
      alleviated the cleaved caspase-3 upregulation caused by OGD. FGF10 treatment also 
      depressed the OGD-induced increase of caspase-3, -8 and -9 activities. At last, 
      we found FGF10 triggered heme oxygenase-1 (HO-1) protein expression rather than 
      hypoxia-inducible factor-1 (HIF-1), AMP-activated protein kinase (AMPK) signaling 
      and extracellular signal-regulated kinases 1/2 (ERK1/2) signaling. Knockdown of 
      HO-1 by siRNA partly abolished the neuroprotection of FGF10 in OGD model. In 
      summary, our observations provide the first evidence for the neuroprotective 
      function of FGF10 against ischemic neuronal injury and suggest that FGF10 may be 
      a promising agent for treatment of ischemic stroke.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Li, Yong-Hua
AU  - Li YH
AD  - Department of Anesthesiology, Changzheng Hospital, 415 Fengyang Road, Second 
      Military Medical University, Shanghai, China.
FAU - Yang, Li-Ye
AU  - Yang LY
AD  - Department of Anesthesiology, Changzheng Hospital, 415 Fengyang Road, Second 
      Military Medical University, Shanghai, China.
FAU - Chen, Wei
AU  - Chen W
AD  - Department of Anesthesiology, Changzheng Hospital, 415 Fengyang Road, Second 
      Military Medical University, Shanghai, China.
FAU - Li, Ying-Ke
AU  - Li YK
AD  - Department of Anesthesiology, Changzheng Hospital, 415 Fengyang Road, Second 
      Military Medical University, Shanghai, China. Electronic address: 
      liyingke6f@126.com.
FAU - Yuan, Hong-Bin
AU  - Yuan HB
AD  - Department of Anesthesiology, Changzheng Hospital, 415 Fengyang Road, Second 
      Military Medical University, Shanghai, China. Electronic address: 
      yuanhongbin6f@126.com.
LA  - eng
PT  - Journal Article
DEP - 20141122
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Blood Glucose)
RN  - 0 (FGF10 protein, human)
RN  - 0 (Fibroblast Growth Factor 10)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 1.14.14.18 (Hmox1 protein, mouse)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Blood Glucose/*metabolism
MH  - Brain Ischemia/pathology
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Cerebral Cortex/metabolism
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Fibroblast Growth Factor 10/*metabolism
MH  - Flow Cytometry
MH  - Heme Oxygenase-1/*metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Membrane Proteins/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neurons/*metabolism
MH  - Oxygen/*metabolism
MH  - PC12 Cells
MH  - RNA, Small Interfering/metabolism
MH  - Rats
MH  - Reactive Oxygen Species/metabolism
MH  - Stroke/pathology
OTO - NOTNLM
OT  - Cerebral ischemia
OT  - FGF10
OT  - HO-1
OT  - Neuroprotection
EDAT- 2014/12/03 06:00
MHDA- 2015/03/11 06:00
CRDT- 2014/12/03 06:00
PHST- 2014/11/12 00:00 [received]
PHST- 2014/11/14 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2015/03/11 06:00 [medline]
AID - S0006-291X(14)02093-2 [pii]
AID - 10.1016/j.bbrc.2014.11.063 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2015 Jan 2;456(1):225-31. doi: 
      10.1016/j.bbrc.2014.11.063. Epub 2014 Nov 22.