PMID- 25446360
OWN - NLM
STAT- MEDLINE
DCOM- 20151230
LR  - 20210109
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 284
DP  - 2015 Jan 22
TI  - Energy status determines hindbrain signal transduction pathway transcriptional 
      reactivity to AMPK in the estradiol-treated ovariectomized female rat.
PG  - 888-899
LID - S0306-4522(14)00949-X [pii]
LID - 10.1016/j.neuroscience.2014.10.068 [doi]
AB  - Dorsal vagal complex (DVC) AMPK regulation of food intake in the 
      estradiol-treated ovariectomized (OVX) female rat is energy state-dependent. 
      Here, RT-PCR array technology was used to identify estradiol-sensitive 
      AMPK-regulated DVC signal transduction pathways that exhibit differential 
      reactivity to sensor activation during energy balance versus imbalance. The AMP 
      mimetic AICAR correspondingly reduced or stimulated cDVC phosphoAMPK (pAMPK) and 
      estrogen receptor-beta (ERbeta) proteins in full-fed (F) versus 12-h food-deprived 
      (D) estradiol-treated ovariectomized (OVX) rats, but elevated ER-alpha (ERalpha) in F 
      only. Estradiol suppressed DVC ERbeta protein and hypoxia, NFkappaB, STAT3, STAT6, and 
      Hedgehog signaling pathway marker genes against oil-implanted OVX controls. 
      F+(A)ICAR and D+(S)aline groups each exhibited further inhibition of NFkappaB, STAT3, 
      and Hedgehog pathway genes, and diminished PPAR, Notch, and STAT5 transcripts 
      versus F+S. Conversely, genes in these six pathways were up-regulated by AICAR 
      treatment of D. Results show that in this animal model, acute AMP augmentation or 
      feeding cessation each inhibit both pAMPK and ERbeta expression, but in combination 
      increase these protein profiles. pAMPK protein and DVC TNF (NFkappaB), SOCS3 
      (JAK/STAT), WNT6 (Hedgehog), and FABP1 (PPAR) mRNAs were down- or upregulated in 
      parallel by AICAR in F versus D states, respectively. Further research is needed 
      to determine the impact of ERbeta on opposing directionality of these responses, and 
      to characterize the role of the aforementioned signaling pathways in hyperphagic 
      responses in the female to AICAR-induced DVC AMPK activation during acute 
      interruption of feeding.
CI  - Copyright (c) 2014 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Ibrahim, B A
AU  - Ibrahim BA
AD  - Department of Basic Pharmaceutical Sciences, School of Pharmacy, University of 
      Louisiana at Monroe, Monroe, LA 71201, United States.
FAU - Alenazi, F S H
AU  - Alenazi FSH
AD  - Department of Basic Pharmaceutical Sciences, School of Pharmacy, University of 
      Louisiana at Monroe, Monroe, LA 71201, United States.
FAU - Briski, K P
AU  - Briski KP
AD  - Department of Basic Pharmaceutical Sciences, School of Pharmacy, University of 
      Louisiana at Monroe, Monroe, LA 71201, United States. Electronic address: 
      briski@ulm.edu.
LA  - eng
GR  - R56 DK085122/DK/NIDDK NIH HHS/United States
PT  - Journal Article
DEP - 20141113
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Estrogens)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/*metabolism
MH  - Animals
MH  - Eating/drug effects/*physiology
MH  - Estradiol/administration & dosage/*metabolism
MH  - Estrogen Receptor alpha/metabolism
MH  - Estrogen Receptor beta/metabolism
MH  - Estrogens/*metabolism/pharmacology
MH  - Female
MH  - Food Deprivation/*physiology
MH  - Ovariectomy
MH  - Rats, Inbred SHR
MH  - Rats, Sprague-Dawley
MH  - Rhombencephalon/drug effects/*physiology
MH  - Signal Transduction/drug effects/physiology
PMC - PMC4267537
MID - NIHMS642422
OTO - NOTNLM
OT  - AICAR
OT  - dorsal vagal complex
OT  - estradiol
OT  - estrogen receptor-alpha
OT  - estrogen receptor-beta
OT  - hedgehog
EDAT- 2014/12/03 06:00
MHDA- 2015/12/31 06:00
PMCR- 2016/01/22
CRDT- 2014/12/03 06:00
PHST- 2014/08/12 00:00 [received]
PHST- 2014/10/13 00:00 [revised]
PHST- 2014/10/31 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2015/12/31 06:00 [medline]
PHST- 2016/01/22 00:00 [pmc-release]
AID - S0306-4522(14)00949-X [pii]
AID - 10.1016/j.neuroscience.2014.10.068 [doi]
PST - ppublish
SO  - Neuroscience. 2015 Jan 22;284:888-899. doi: 10.1016/j.neuroscience.2014.10.068. 
      Epub 2014 Nov 13.