PMID- 25447771
OWN - NLM
STAT- MEDLINE
DCOM- 20150826
LR  - 20141220
IS  - 1879-3150 (Electronic)
IS  - 0041-0101 (Linking)
VI  - 93
DP  - 2015 Jan
TI  - Nicotinic acetylcholine receptor alpha7 subunit is involved in the cobratoxin-induced 
      antinociception in an animal model of neuropathic pain.
PG  - 31-6
LID - S0041-0101(14)00583-2 [pii]
LID - 10.1016/j.toxicon.2014.11.222 [doi]
AB  - In this study we report that cobratoxin (CbTX), a long-chain postsynaptic 
      alpha-neurotoxin isolated from the Thailand cobra, Naja naja kaouthia, has 
      antinociceptive effect in rats with neuropathic pain. The neuropathic pain model 
      was established in rats with partial sciatic nerve ligature (PSNL) method. The 
      pain response was examined behaviorally with mechanical paw withdrawal and 
      thermal paw withdrawal method. Different doses (0.56, 1.12 and 4.50 mug/kg) of 
      CbTX were injected intrathecally. Injection of CbTX resulted in a significant 
      dose-dependent antinociception as evidenced by increased mechanical withdrawal 
      threshold and thermal withdrawal latency. CbTX also induces a significant 
      dose-dependent inhibition of pain-evoked unit discharges of thalamic 
      parafascicular neurons. Both the behavioral mechanical and thermal 
      antinociception and the inhibition of pain-evoked discharges of neurons in 
      thalamic parafascicular nucleus in PSNL model could be mimicked by PUN282987, 
      selective alpha7 nicotinic AChR (alpha7 nAChR) agonist and reversed by methyllycaconitine 
      (MLA) selective alpha7 nAChR antagonist. In summary, these results suggested that 
      AChR alpha7 subunit was involved in the antinociceptive action of CbTX for 
      neuropathic pain and might be the candidate target for analgesic drug design.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Gong, Shan
AU  - Gong S
AD  - Department of Physiology and Neurobiology, Key Laboratory of Pain Research and 
      Therapy, Medical College of Soochow University, PR China.
FAU - Liang, Qian
AU  - Liang Q
AD  - Department of Physiology and Neurobiology, Key Laboratory of Pain Research and 
      Therapy, Medical College of Soochow University, PR China.
FAU - Zhu, Qi
AU  - Zhu Q
AD  - Department of Physiology and Neurobiology, Key Laboratory of Pain Research and 
      Therapy, Medical College of Soochow University, PR China.
FAU - Ding, Dayong
AU  - Ding D
AD  - Department of Physiology and Neurobiology, Key Laboratory of Pain Research and 
      Therapy, Medical College of Soochow University, PR China.
FAU - Yin, Qizhang
AU  - Yin Q
AD  - Department of Physiology and Neurobiology, Key Laboratory of Pain Research and 
      Therapy, Medical College of Soochow University, PR China.
FAU - Tao, Jin
AU  - Tao J
AD  - Department of Physiology and Neurobiology, Key Laboratory of Pain Research and 
      Therapy, Medical College of Soochow University, PR China.
FAU - Jiang, Xinghong
AU  - Jiang X
AD  - Department of Physiology and Neurobiology, Key Laboratory of Pain Research and 
      Therapy, Medical College of Soochow University, PR China. Electronic address: 
      jiangxinghong@suda.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141106
PL  - England
TA  - Toxicon
JT  - Toxicon : official journal of the International Society on Toxinology
JID - 1307333
RN  - 0 (Analgesics)
RN  - 0 (Cobra Neurotoxin Proteins)
RN  - 0 (alpha7 Nicotinic Acetylcholine Receptor)
SB  - IM
MH  - Analgesics/*pharmacology
MH  - Analysis of Variance
MH  - Animals
MH  - Cobra Neurotoxin Proteins/administration & dosage/*metabolism/*pharmacology
MH  - *Disease Models, Animal
MH  - Injections, Spinal
MH  - Male
MH  - Neuralgia/*drug therapy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - alpha7 Nicotinic Acetylcholine Receptor/*metabolism
OTO - NOTNLM
OT  - Antinociception
OT  - Cobratoxin
OT  - Thalamic parafascicular nucleus
OT  - alpha7 nicotinic acetylcholine receptor
EDAT- 2014/12/03 06:00
MHDA- 2015/08/27 06:00
CRDT- 2014/12/03 06:00
PHST- 2014/01/27 00:00 [received]
PHST- 2014/05/19 00:00 [revised]
PHST- 2014/11/05 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2015/08/27 06:00 [medline]
AID - S0041-0101(14)00583-2 [pii]
AID - 10.1016/j.toxicon.2014.11.222 [doi]
PST - ppublish
SO  - Toxicon. 2015 Jan;93:31-6. doi: 10.1016/j.toxicon.2014.11.222. Epub 2014 Nov 6.