PMID- 25448704
OWN - NLM
STAT- MEDLINE
DCOM- 20150916
LR  - 20141229
IS  - 1879-0542 (Electronic)
IS  - 0165-2478 (Linking)
VI  - 163
IP  - 1
DP  - 2015 Jan
TI  - Single-chain anti-idiotypic antibody retains its specificity to porcine 
      reproductive and respiratory syndrome virus GP5.
PG  - 8-13
LID - S0165-2478(14)00273-9 [pii]
LID - 10.1016/j.imlet.2014.11.011 [doi]
AB  - Monoclonal anti-idiotypic antibody (Mab2-5G2) raised against idiotypic antibodies 
      to membrane glycoprotein GP5 of porcine reproductive and respiratory syndrome 
      virus (PRRSV). The variable regions of the heavy chain (VH) and light chain (VL) 
      of Mab2-5G2 were cloned and connected with a (Gly4Ser)3 linker. The recombinant 
      scFv gene was cloned into the pEasy-E1 vector and expressed in E. coli as 
      inclusion bodies. The expressed scFv-His proteins renatured in a pH and urea 
      gradient buffer retained the same immunological properties as that of Mab2-5G2. 
      Renatured scFv-His protein retained the same characteristics as that of Mab2-5G2 
      by recognizing and binding to Marc-145 cells. Furthermore, renatured scFv-His 
      along with Mab2-5G2 were used to immunize rabbits to produce anti-anti-idiotypic 
      antibodies (Ab3) that neutralized PRRSV infection of Marc-145 cells. These 
      results demonstrated that the expressed scFv-His protein possessed the same 
      characteristics of Mab2-5G2 and will be suitable for future investigations of 
      Mab2-5G2 antibody structure and its ability to interact with potential PRRSV 
      cellular receptor as well as immunological properties against PRRSV infection.
CI  - Copyright (c) 2014 European Federation of Immunological Societies. Published by 
      Elsevier B.V. All rights reserved.
FAU - Yu, Ying
AU  - Yu Y
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station 
      of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of 
      Agriculture, Yangling, Shaanxi 712100, China.
FAU - Wang, Gang
AU  - Wang G
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agriculture Science, Harbin 150001, China.
FAU - Li, Qiongyi
AU  - Li Q
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station 
      of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of 
      Agriculture, Yangling, Shaanxi 712100, China.
FAU - Du, Yongkun
AU  - Du Y
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station 
      of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of 
      Agriculture, Yangling, Shaanxi 712100, China.
FAU - Du, Taofeng
AU  - Du T
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station 
      of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of 
      Agriculture, Yangling, Shaanxi 712100, China.
FAU - Mu, Yang
AU  - Mu Y
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station 
      of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of 
      Agriculture, Yangling, Shaanxi 712100, China.
FAU - Xiao, Shuqi
AU  - Xiao S
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station 
      of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of 
      Agriculture, Yangling, Shaanxi 712100, China.
FAU - Zhao, Qin
AU  - Zhao Q
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station 
      of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of 
      Agriculture, Yangling, Shaanxi 712100, China.
FAU - Wang, Chengbao
AU  - Wang C
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station 
      of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of 
      Agriculture, Yangling, Shaanxi 712100, China.
FAU - Sun, Yani
AU  - Sun Y
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station 
      of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of 
      Agriculture, Yangling, Shaanxi 712100, China.
FAU - Xu, Xingang
AU  - Xu X
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station 
      of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of 
      Agriculture, Yangling, Shaanxi 712100, China.
FAU - Zhang, Gaiping
AU  - Zhang G
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Henan Agricultural University, Zhengzhou, Henan 450002, China.
FAU - Hsu, Walter H
AU  - Hsu WH
AD  - Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State 
      University, Ames, IA, USA.
FAU - Cai, Xuehui
AU  - Cai X
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agriculture Science, Harbin 150001, China. 
      Electronic address: cai139@hvri.ac.cn.
FAU - Zhou, En-Min
AU  - Zhou EM
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station 
      of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of 
      Agriculture, Yangling, Shaanxi 712100, China. Electronic address: 
      zhouem@nwsuaf.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141124
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Single-Chain Antibodies)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - *Antibodies, Neutralizing/genetics/immunology/pharmacology
MH  - *Antibodies, Viral/genetics/immunology/pharmacology
MH  - Antibody Specificity/*genetics
MH  - Cell Line
MH  - Mice
MH  - Porcine Reproductive and Respiratory Syndrome/drug 
      therapy/genetics/immunology/pathology
MH  - *Porcine respiratory and reproductive syndrome virus/genetics/immunology
MH  - Rabbits
MH  - *Single-Chain Antibodies/genetics/immunology/pharmacology
MH  - Swine
MH  - *Viral Envelope Proteins/genetics/immunology
OTO - NOTNLM
OT  - Anti-idiotype
OT  - PRRSV
OT  - Single-chain antibody
EDAT- 2014/12/03 06:00
MHDA- 2015/09/17 06:00
CRDT- 2014/12/03 06:00
PHST- 2014/09/06 00:00 [received]
PHST- 2014/10/30 00:00 [revised]
PHST- 2014/11/14 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2015/09/17 06:00 [medline]
AID - S0165-2478(14)00273-9 [pii]
AID - 10.1016/j.imlet.2014.11.011 [doi]
PST - ppublish
SO  - Immunol Lett. 2015 Jan;163(1):8-13. doi: 10.1016/j.imlet.2014.11.011. Epub 2014 
      Nov 24.