PMID- 25449571
OWN - NLM
STAT- MEDLINE
DCOM- 20150723
LR  - 20200407
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 195
DP  - 2015 Jan 2
TI  - The DEAD-box RNA helicase 5 positively regulates the replication of porcine 
      reproductive and respiratory syndrome virus by interacting with viral Nsp9 in 
      vitro.
PG  - 217-24
LID - S0168-1702(14)00438-9 [pii]
LID - 10.1016/j.virusres.2014.10.021 [doi]
AB  - The nonstructural protein 9 (Nsp9) of porcine reproductive and respiratory 
      syndrome virus (PRRSV) has been recognized to play important roles in viral 
      replication. The present study first screened that the DEAD-box RNA helicase 5 
      (DDX5) was a cellular protein interacting with the Nsp9 of PRRSV by a yeast 
      two-hybrid method in a pulmonary alveolar macrophages (PAMs) cDNA library. Next, 
      DDX5 was shown to interact with viral Nsp9 in the co-transfected HEK293 cells 
      with the DDX5- and Nsp9-expressing plasmids, and the interaction between 
      endogenous DDX5 and Nsp9 was also confirmed in MARC-145 cells infected with the 
      Nsp9-expressing lentiviruses. Then, the interacting domains between DDX5 and Nsp9 
      were determined to be the DEXDc and HELICc domains in DDX5 and the RdRp domain in 
      Nsp9, respectively. Moreover, in the HEK293 cells, MARC-145 cells and PAM cell 
      lines co-transfected with the DDX5- and Nsp9-expressing plasmids, Nsp9 was shown 
      to co-localize with DDX5 in the cytoplasm with a perinuclear pattern, and 
      meanwhile in PRRSV-infected MARC-145 cells and PAMs, endogenous DDX5 was also 
      found to co-localize with Nsp9. Finally, silencing the DDX5 gene in MARC-145 
      cells significantly impacted the replication of PRRSV, and while the 
      over-expression of DDX5 could slightly enhance viral replication. These findings 
      indicate that DDX5 positively regulates the replication of PRRSV via its 
      interaction with viral Nsp9 in vitro.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Zhao, Shuangcheng
AU  - Zhao S
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing 100193, People's Republic of China.
FAU - Ge, Xinna
AU  - Ge X
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing 100193, People's Republic of China.
FAU - Wang, Xiaolong
AU  - Wang X
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing 100193, People's Republic of China.
FAU - Liu, Aijing
AU  - Liu A
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing 100193, People's Republic of China.
FAU - Guo, Xin
AU  - Guo X
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing 100193, People's Republic of China.
FAU - Zhou, Lei
AU  - Zhou L
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing 100193, People's Republic of China.
FAU - Yu, Kangzhen
AU  - Yu K
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing 100193, People's Republic of China; The 
      Ministry of Agriculture of the People's Republic of China, Beijing 100026, 
      People's Republic of China.
FAU - Yang, Hanchun
AU  - Yang H
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing 100193, People's Republic of China. 
      Electronic address: yanghanchun1@cau.edu.cn.
LA  - eng
SI  - GENBANK/KM434334
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141101
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Viral Nonstructural Proteins)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - DEAD-box RNA Helicases/*metabolism
MH  - Humans
MH  - Macrophages, Alveolar/virology
MH  - Molecular Sequence Data
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Protein Binding
MH  - Protein Interaction Domains and Motifs
MH  - Protein Interaction Mapping
MH  - Sequence Analysis, DNA
MH  - Swine
MH  - Two-Hybrid System Techniques
MH  - Viral Nonstructural Proteins/*metabolism
MH  - *Virus Replication
PMC - PMC7114378
OTO - NOTNLM
OT  - DEAD (Asp-Glu-Ala-Asp)-box RNA helicase 5 (DDX5)
OT  - Interaction
OT  - Nonstructural protein 9 (Nsp9)
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - Replication
EDAT- 2014/12/03 06:00
MHDA- 2015/07/24 06:00
PMCR- 2014/11/01
CRDT- 2014/12/03 06:00
PHST- 2014/09/01 00:00 [received]
PHST- 2014/10/24 00:00 [revised]
PHST- 2014/10/27 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2015/07/24 06:00 [medline]
PHST- 2014/11/01 00:00 [pmc-release]
AID - S0168-1702(14)00438-9 [pii]
AID - 10.1016/j.virusres.2014.10.021 [doi]
PST - ppublish
SO  - Virus Res. 2015 Jan 2;195:217-24. doi: 10.1016/j.virusres.2014.10.021. Epub 2014 
      Nov 1.