PMID- 25449888 OWN - NLM STAT- MEDLINE DCOM- 20150804 LR - 20141209 IS - 1872-6240 (Electronic) IS - 0006-8993 (Linking) VI - 1594 DP - 2015 Jan 12 TI - Human umbilical cord mesenchymal stem cells protect against ischemic brain injury in mouse by regulating peripheral immunoinflammation. PG - 293-304 LID - S0006-8993(14)01487-5 [pii] LID - 10.1016/j.brainres.2014.10.065 [doi] AB - Current treatments for ischemic stroke are limited, stem cell transplantation offers great potential as a therapeutic strategy. The present study was undertaken to determine whether human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) could improve brain injury after middle cerebral artery occlusion (MCAO) through modulating peripheral immunoinflammation. The study showed that neurological deficit was ameliorated and brain edema, infarct volume was significantly decreased from 72 h to 1 week post-MCAO with hUC-MSCs treatment via tail vein injection within 30 mins after stroke; hUC-MSCs attenuated the levels of inflammatory factors including IL-1, TNF-alpha, IL-23, IL-17 and IL-10 in peripheral blood serum and ischemia hemisphere after stroke; hUC-MSCs significantly decreased the level of Th17 cells at 24h and increased the level of Tregs at 72 h post-MCAO in peripheral immune system; the level of TGF-beta in blood serum was enhanced by hUC-MSCs. In conclusion, our findings suggested that hUC-MSCs had neuroprotection in MCAO mice by TGF-beta modulating peripheral immune and hUC-MSCs may be as a potential therapy for ischemic stroke. CI - Copyright (c) 2014 Elsevier B.V. All rights reserved. FAU - Cheng, Qiantao AU - Cheng Q AD - Department of Neurology, Affiliated Drum Tower Hopital, Nanjing University Medical School, PR China; Department of Neurology, Affiliated Huai׳an First People׳s Hospital of Nanjing Medical University, Huai׳an, Jiangsu Province, PR China. FAU - Zhang, Zhuo AU - Zhang Z AD - Department of Neurology, Affiliated Drum Tower Hopital, Nanjing University Medical School, PR China; Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing, PR China; Jiangsu Province Stroke Center for Diagnosis and Therapy, PR China. FAU - Zhang, Shenyang AU - Zhang S AD - Department of Neurology, Affiliated Drum Tower Hopital, Nanjing University Medical School, PR China; Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing, PR China; Jiangsu Province Stroke Center for Diagnosis and Therapy, PR China. FAU - Yang, Hui AU - Yang H AD - Department of Neurology, Affiliated Drum Tower Hopital, Nanjing University Medical School, PR China; Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing, PR China; Jiangsu Province Stroke Center for Diagnosis and Therapy, PR China. FAU - Zhang, Xin AU - Zhang X AD - Department of Neurology, Affiliated Drum Tower Hopital, Nanjing University Medical School, PR China; Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing, PR China; Jiangsu Province Stroke Center for Diagnosis and Therapy, PR China. FAU - Pan, Jie AU - Pan J AD - Department of Neurology, Affiliated Drum Tower Hopital, Nanjing University Medical School, PR China; Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing, PR China; Jiangsu Province Stroke Center for Diagnosis and Therapy, PR China. FAU - Weng, Leihua AU - Weng L AD - Department of Neurology, Affiliated Drum Tower Hopital, Nanjing University Medical School, PR China; Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing, PR China; Jiangsu Province Stroke Center for Diagnosis and Therapy, PR China. FAU - Sha, Dujuan AU - Sha D AD - Department of Neurology, Affiliated Drum Tower Hopital, Nanjing University Medical School, PR China; Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing, PR China; Jiangsu Province Stroke Center for Diagnosis and Therapy, PR China. FAU - Zhu, Min AU - Zhu M AD - Department of Neurology, Affiliated Drum Tower Hopital, Nanjing University Medical School, PR China; Department of Neurology and Rehabilitation, Nanjing Children׳s Hospital of Nanjing Medical University, Nanjing, PR China. FAU - Hu, Xiang AU - Hu X AD - Shenzhen Beike Stem cell Engineering Institute, PR China. FAU - Xu, Yun AU - Xu Y AD - Department of Neurology, Affiliated Drum Tower Hopital, Nanjing University Medical School, PR China; Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing, PR China; Jiangsu Province Stroke Center for Diagnosis and Therapy, PR China; Nanjing Neuropsychiatry Clinic Medical Center, PR China. Electronic address: xuyun20042001@aliyun.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20141106 PL - Netherlands TA - Brain Res JT - Brain research JID - 0045503 SB - IM MH - Animals MH - Brain Ischemia/*immunology/*pathology MH - Cell Differentiation/immunology MH - *Cord Blood Stem Cell Transplantation MH - Disease Models, Animal MH - Enzyme-Linked Immunosorbent Assay MH - Flow Cytometry MH - Humans MH - Inflammation/immunology/pathology MH - Male MH - *Mesenchymal Stem Cell Transplantation MH - Mice MH - Neuroimmunomodulation/*immunology MH - Real-Time Polymerase Chain Reaction MH - T-Lymphocyte Subsets/cytology/immunology OTO - NOTNLM OT - Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) OT - Neuroimmunology OT - Stroke OT - Transplantation EDAT- 2014/12/03 06:00 MHDA- 2015/08/05 06:00 CRDT- 2014/12/03 06:00 PHST- 2014/08/06 00:00 [received] PHST- 2014/10/21 00:00 [revised] PHST- 2014/10/28 00:00 [accepted] PHST- 2014/12/03 06:00 [entrez] PHST- 2014/12/03 06:00 [pubmed] PHST- 2015/08/05 06:00 [medline] AID - S0006-8993(14)01487-5 [pii] AID - 10.1016/j.brainres.2014.10.065 [doi] PST - ppublish SO - Brain Res. 2015 Jan 12;1594:293-304. doi: 10.1016/j.brainres.2014.10.065. Epub 2014 Nov 6.