PMID- 25450314 OWN - NLM STAT- MEDLINE DCOM- 20151120 LR - 20220408 IS - 1872-8111 (Electronic) IS - 0168-0102 (Linking) VI - 91 DP - 2015 Feb TI - BDNF promoter I methylation correlates between post-mortem human peripheral and brain tissues. PG - 1-7 LID - S0168-0102(14)00226-0 [pii] LID - 10.1016/j.neures.2014.10.003 [doi] AB - Several psychiatric disorders have been associated with CpG methylation changes in CG rich promoters of the brain-derived neurotrophic factor (BDNF) mainly by extracting DNA from peripheral blood cells. Whether changes in peripheral DNA methylation can be used as a proxy for brain-specific alterations remains an open question. In this study we aimed to compare DNA methylation levels in BDNF promoter regions in human blood cells, muscle and brain regions using bisulfite-pyrosequencing. We found a significant correlation between the levels of BDNF promoter I methylation measured in quadriceps and vPFC tissues extracted from the same individuals (n = 98, Pearson, r = 0.48, p = 4.5 x 10(-7)). In the hippocampus, BDNF promoter I and IV methylation levels were strongly correlated (Pearson, n = 37, r = 0.74, p = 1.4 x 10(-7)). We found evidence for sex-dependent effect on BDNF promoter methylation levels in the various tissues and blood samples. Taken together, these data indicate a strong intra-individual correlation between peripheral and brain tissue. They also suggest that sex determines methylation patterns in BDNF promoter region across different types of tissue, including muscle, brain, and blood. CI - Copyright (c) 2014 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved. FAU - Stenz, Ludwig AU - Stenz L AD - Department of Psychiatry, University of Geneva, Switzerland; Department of Basic Neurosciences, University of Geneva Medical School, Geneva, Switzerland. Electronic address: ludwig.stenz@unige.ch. FAU - Zewdie, Seblewongel AU - Zewdie S AD - Department of Psychiatry, University of Geneva, Switzerland; Department of Basic Neurosciences, University of Geneva Medical School, Geneva, Switzerland. FAU - Laforge-Escarra, Terese AU - Laforge-Escarra T AD - Department of Genetic and Laboratory Medicine, Geneva University Hospital, 1211 Geneva 14, Switzerland. FAU - Prados, Julien AU - Prados J AD - Department of Psychiatry, University of Geneva, Switzerland; Department of Basic Neurosciences, University of Geneva Medical School, Geneva, Switzerland. FAU - La Harpe, Romano AU - La Harpe R AD - Institute of Forensic Medicine, University of Geneva, Switzerland. FAU - Dayer, Alexandre AU - Dayer A AD - Department of Mental Health and Psychiatry, University Hospital of Geneva, Geneva, Switzerland; Department of Basic Neurosciences, University of Geneva Medical School, Geneva, Switzerland. FAU - Paoloni-Giacobino, Ariane AU - Paoloni-Giacobino A AD - Department of Genetic and Laboratory Medicine, Geneva University Hospital, 1211 Geneva 14, Switzerland. FAU - Perroud, Nader AU - Perroud N AD - Department of Psychiatry, University of Geneva, Switzerland; Department of Mental Health and Psychiatry, University Hospital of Geneva, Geneva, Switzerland. FAU - Aubry, Jean-Michel AU - Aubry JM AD - Department of Psychiatry, University of Geneva, Switzerland; Department of Mental Health and Psychiatry, University Hospital of Geneva, Geneva, Switzerland. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20141017 PL - Ireland TA - Neurosci Res JT - Neuroscience research JID - 8500749 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 7171WSG8A2 (BDNF protein, human) SB - IM MH - Autopsy MH - Bipolar Disorder/genetics/metabolism MH - Blood Cells/metabolism MH - Brain/*metabolism MH - Brain-Derived Neurotrophic Factor/blood/genetics/*metabolism MH - *DNA Methylation MH - Female MH - Humans MH - Male MH - Muscle, Skeletal/*metabolism MH - Organ Specificity MH - *Promoter Regions, Genetic MH - Sex Factors OTO - NOTNLM OT - BDNF OT - Epigenetics OT - Methylation OT - Prefrontal cortex OT - Promoter OT - Quadriceps EDAT- 2014/12/03 06:00 MHDA- 2015/12/15 06:00 CRDT- 2014/12/03 06:00 PHST- 2014/06/19 00:00 [received] PHST- 2014/08/25 00:00 [revised] PHST- 2014/10/06 00:00 [accepted] PHST- 2014/12/03 06:00 [entrez] PHST- 2014/12/03 06:00 [pubmed] PHST- 2015/12/15 06:00 [medline] AID - S0168-0102(14)00226-0 [pii] AID - 10.1016/j.neures.2014.10.003 [doi] PST - ppublish SO - Neurosci Res. 2015 Feb;91:1-7. doi: 10.1016/j.neures.2014.10.003. Epub 2014 Oct 17.