PMID- 25450462
OWN - NLM
STAT- MEDLINE
DCOM- 20150915
LR  - 20141216
IS  - 1878-5875 (Electronic)
IS  - 1357-2725 (Linking)
VI  - 57
DP  - 2014 Dec
TI  - Power frequency magnetic fields induced reactive oxygen species-related autophagy 
      in mouse embryonic fibroblasts.
PG  - 108-14
LID - S1357-2725(14)00324-0 [pii]
LID - 10.1016/j.biocel.2014.10.013 [doi]
AB  - Power frequency magnetic fields (PFMF) have been reported to affect several 
      cellular functions, such as cell proliferation and apoptosis. In this study, we 
      investigated the effects of PFMF on mouse embryonic fibroblasts (MEF) autophagy. 
      After cells were exposed to 50 Hz PFMF at 2 mT for 0.5 h, 2 h, 6 h, 12 h, and 24 
      h, we observed a significant increase in autophagic markers at 6 h, including (i) 
      higher microtubule-associated protein 1 light chain 3-II (LC3-II), (ii) the 
      increased formation of GFP-LC3 puncta, and (iii) increased numbers of autophagic 
      vacuoles under transmission electron microscope. Moreover, we provide convincing 
      evidence using chloroquine (CQ) that the increase of autophagic markers was the 
      result of enhanced autophagic flux and not the suppression of lysosomal function. 
      In a search for molecular mechanisms underlying PFMF-mediated autophagy, we 
      observe that the autophagic process involved reactive oxygen species (ROS) and 
      was independent of the mammalian target of rapamycin (mTOR) signaling pathway.
CI  - Copyright (c) 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Chen, Yanfeng
AU  - Chen Y
AD  - Bioelectromagnetics Laboratory, School of Medicine, Zhejiang University, 866 
      Yuhangtang Road, Hangzhou, 310058, China.
FAU - Hong, Ling
AU  - Hong L
AD  - Bioelectromagnetics Laboratory, School of Medicine, Zhejiang University, 866 
      Yuhangtang Road, Hangzhou, 310058, China.
FAU - Zeng, Ying
AU  - Zeng Y
AD  - Bioelectromagnetics Laboratory, School of Medicine, Zhejiang University, 866 
      Yuhangtang Road, Hangzhou, 310058, China.
FAU - Shen, Yunyun
AU  - Shen Y
AD  - Bioelectromagnetics Laboratory, School of Medicine, Zhejiang University, 866 
      Yuhangtang Road, Hangzhou, 310058, China.
FAU - Zeng, Qunli
AU  - Zeng Q
AD  - Bioelectromagnetics Laboratory, School of Medicine, Zhejiang University, 866 
      Yuhangtang Road, Hangzhou, 310058, China. Electronic address: zengql@zju.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141019
PL  - Netherlands
TA  - Int J Biochem Cell Biol
JT  - The international journal of biochemistry & cell biology
JID - 9508482
RN  - 0 (Reactive Oxygen Species)
SB  - IM
MH  - Animals
MH  - Apoptosis/*radiation effects
MH  - Autophagy/*drug effects
MH  - Cells, Cultured
MH  - Embryo, Mammalian/cytology
MH  - Fibroblasts/cytology/*metabolism/*radiation effects
MH  - *Magnetic Fields
MH  - Mice
MH  - Reactive Oxygen Species/*metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - Autophagy
OT  - Mouse embryonic fibroblasts
OT  - Power frequency magnetic fields
OT  - Reactive oxygen species
EDAT- 2014/12/03 06:00
MHDA- 2015/09/16 06:00
CRDT- 2014/12/03 06:00
PHST- 2014/06/24 00:00 [received]
PHST- 2014/09/30 00:00 [revised]
PHST- 2014/10/08 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2015/09/16 06:00 [medline]
AID - S1357-2725(14)00324-0 [pii]
AID - 10.1016/j.biocel.2014.10.013 [doi]
PST - ppublish
SO  - Int J Biochem Cell Biol. 2014 Dec;57:108-14. doi: 10.1016/j.biocel.2014.10.013. 
      Epub 2014 Oct 19.