PMID- 25451069
OWN - NLM
STAT- MEDLINE
DCOM- 20150723
LR  - 20141208
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 195
DP  - 2015 Jan 2
TI  - Porcine reproductive and respiratory syndrome virus replication and quasispecies 
      evolution in pigs that lack adaptive immunity.
PG  - 246-9
LID - S0168-1702(14)00411-0 [pii]
LID - 10.1016/j.virusres.2014.10.006 [doi]
AB  - The replication of porcine reproductive and respiratory syndrome virus (PRRSV) 
      was studied in a line of pigs possessing a severe combined immunodeficiency 
      (SCID). Real-time RT-PCR revealed a unique course of infection for the SCID 
      group. During the course of infection, viremia was initially significantly lower 
      than normal littermates, but by 21 days was significantly elevated. Deep 
      sequencing of the viral structural genes at days 11 and 21 identified seven amino 
      acid substitutions in both normal and SCID pigs. The most significant change was 
      a W99R substitution in GP2, which was present in the inoculum at a frequency of 
      35%, but eventually disappeared from all pigs regardless of immune status. 
      Therefore, amino acid substitutions that appear during acute infection are likely 
      the result of the adaptation of the virus to replication in pigs and not immune 
      selection.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Chen, Nanhua
AU  - Chen N
AD  - Department of Diagnostic Medicine and Pathobiology, College of Veterinary 
      Medicine, Kansas State University, Manhattan, KS 66506, United States.
FAU - Dekkers, Jack C M
AU  - Dekkers JC
AD  - Department of Animal Science, Iowa State University, Ames, IA 50011-3150, United 
      States.
FAU - Ewen, Catherine L
AU  - Ewen CL
AD  - Department of Diagnostic Medicine and Pathobiology, College of Veterinary 
      Medicine, Kansas State University, Manhattan, KS 66506, United States.
FAU - Rowland, Raymond R R
AU  - Rowland RR
AD  - Department of Diagnostic Medicine and Pathobiology, College of Veterinary 
      Medicine, Kansas State University, Manhattan, KS 66506, United States. Electronic 
      address: browland@vet.ksu.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141014
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Adaptive Immunity
MH  - Amino Acid Substitution
MH  - Animals
MH  - *Genetic Variation
MH  - High-Throughput Nucleotide Sequencing
MH  - Porcine respiratory and reproductive syndrome 
      virus/classification/*genetics/*physiology
MH  - RNA, Viral/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Severe Combined Immunodeficiency
MH  - Swine
MH  - Time Factors
MH  - Viral Load
MH  - Viremia
MH  - *Virus Replication
OTO - NOTNLM
OT  - Deep sequencing
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - Quasispecies
OT  - Severe combined immunodeficiency (SCID)
EDAT- 2014/12/03 06:00
MHDA- 2015/07/24 06:00
CRDT- 2014/12/03 06:00
PHST- 2014/08/28 00:00 [received]
PHST- 2014/10/03 00:00 [revised]
PHST- 2014/10/06 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2015/07/24 06:00 [medline]
AID - S0168-1702(14)00411-0 [pii]
AID - 10.1016/j.virusres.2014.10.006 [doi]
PST - ppublish
SO  - Virus Res. 2015 Jan 2;195:246-9. doi: 10.1016/j.virusres.2014.10.006. Epub 2014 
      Oct 14.