PMID- 25451226
OWN - NLM
STAT- MEDLINE
DCOM- 20150217
LR  - 20181113
IS  - 1873-3468 (Electronic)
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 588
IP  - 24
DP  - 2014 Dec 20
TI  - Identification of the WNT1 residues required for palmitoylation by Porcupine.
PG  - 4815-24
LID - S0014-5793(14)00821-7 [pii]
LID - 10.1016/j.febslet.2014.11.016 [doi]
AB  - The post-translational palmitoylation of WNT morphogens is critical for proper 
      signaling during embryogenesis and adult homeostasis. The addition of palmitoyl 
      groups to WNT proteins is catalyzed by Porcupine (PORCN). However, the Wnt amino 
      acid residues required for recognition and palmitoylation by PORCN have not been 
      fully characterized. We show that WNT1 residues 214-234 are sufficient for 
      PORCN-dependent palmitoylation of Ser224. Substitution of Ser224 with Thr, but 
      not Cys, is tolerated in palmitoylation and biological assays. Our data highlight 
      the importance of palmitoylation for WNT1 activity and establish PORCN as an 
      O-acyl transferase for WNT1.
CI  - Copyright (c) 2014 Federation of European Biochemical Societies. Published by 
      Elsevier B.V. All rights reserved.
FAU - Miranda, M
AU  - Miranda M
AD  - Department of Biology, San Francisco State University, 1600 Holloway Avenue, San 
      Francisco, CA 94132, USA.
FAU - Galli, L M
AU  - Galli LM
AD  - Department of Biology, San Francisco State University, 1600 Holloway Avenue, San 
      Francisco, CA 94132, USA.
FAU - Enriquez, M
AU  - Enriquez M
AD  - Department of Biology, San Francisco State University, 1600 Holloway Avenue, San 
      Francisco, CA 94132, USA.
FAU - Szabo, L A
AU  - Szabo LA
AD  - Department of Biology, San Francisco State University, 1600 Holloway Avenue, San 
      Francisco, CA 94132, USA.
FAU - Gao, X
AU  - Gao X
AD  - Department of Early Discovery Biochemistry, Genentech, 1 DNA Way, South San 
      Francisco, CA 94080, USA.
FAU - Hannoush, R N
AU  - Hannoush RN
AD  - Department of Early Discovery Biochemistry, Genentech, 1 DNA Way, South San 
      Francisco, CA 94080, USA.
FAU - Burrus, L W
AU  - Burrus LW
AD  - Department of Biology, San Francisco State University, 1600 Holloway Avenue, San 
      Francisco, CA 94132, USA. Electronic address: LBurrus@sfsu.edu.
LA  - eng
GR  - 1R15HD070206-01A1/HD/NICHD NIH HHS/United States
GR  - P20MD000262/MD/NIMHD NIH HHS/United States
GR  - 2S06 GM52588/GM/NIGMS NIH HHS/United States
GR  - S06 GM052588/GM/NIGMS NIH HHS/United States
GR  - P20 MD000262/MD/NIMHD NIH HHS/United States
GR  - R15 HD070206/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20141120
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Membrane Proteins)
RN  - 0 (Wnt1 Protein)
RN  - 2V16EO95H1 (Palmitic Acid)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.3.1.- (PORCN protein, human)
SB  - IM
MH  - Acyltransferases/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chick Embryo
MH  - Chickens
MH  - HEK293 Cells
MH  - Humans
MH  - Membrane Proteins/*metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Palmitic Acid/*metabolism
MH  - *Protein Processing, Post-Translational
MH  - Substrate Specificity
MH  - Wnt1 Protein/*chemistry/*metabolism
PMC - PMC4268045
MID - NIHMS643860
OTO - NOTNLM
OT  - Chick
OT  - Membrane-bound O-acyl transferase
OT  - Palmitoylation
OT  - Porcupine
OT  - Spinal cord
OT  - WNT1
EDAT- 2014/12/03 06:00
MHDA- 2015/02/18 06:00
PMCR- 2015/12/20
CRDT- 2014/12/03 06:00
PHST- 2014/09/27 00:00 [received]
PHST- 2014/10/27 00:00 [revised]
PHST- 2014/11/12 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2015/02/18 06:00 [medline]
PHST- 2015/12/20 00:00 [pmc-release]
AID - S0014-5793(14)00821-7 [pii]
AID - 10.1016/j.febslet.2014.11.016 [doi]
PST - ppublish
SO  - FEBS Lett. 2014 Dec 20;588(24):4815-24. doi: 10.1016/j.febslet.2014.11.016. Epub 
      2014 Nov 20.