PMID- 25451288
OWN - NLM
STAT- MEDLINE
DCOM- 20151230
LR  - 20231213
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 284
DP  - 2015 Jan 22
TI  - Effect of actual long-term spaceflight on BDNF, TrkB, p75, BAX and BCL-XL genes 
      expression in mouse brain regions.
PG  - 730-736
LID - S0306-4522(14)00917-8 [pii]
LID - 10.1016/j.neuroscience.2014.10.045 [doi]
AB  - Mice of C57BL/6J strain were exposed to 1-month spaceflight on Russian 
      biosatellite Bion-M1 to determine the effect of long-term actual spaceflight on 
      the expression of genes involved in the processes of neurogenesis and apoptosis. 
      Specifically, we focused on the genes encoding proapoptotic factor BAX, 
      antiapoptotic factor BCL-XL, brain-derived neurotrophic factor (BDNF) and BDNF 
      receptors TrkB and p75. Spaceflight reduced the expression of the antiapoptotic 
      BCL-XL gene in the striatum and hypothalamus, but increased it in the 
      hippocampus. To estimate environmental stress contribution into spaceflight 
      effects we analyzed spaceflight-responsive genes in mice housed for 1 month on 
      Earth in the same shuttle cabins that were used for spaceflight, and in mice of 
      the laboratory control group. It was shown that 1-month shuttle cabin housing 
      decreased BCL-XL gene expression in the striatum but failed to alter BCL-XL mRNA 
      levels in the hippocampus or hypothalamus. Spaceflight failed to alter the 
      expression of the proapoptotic BAX gene in all investigated brain structures, 
      although the insignificant increase of the BAX mRNA level in the hippocampus of 
      spaceflight mice was found. At the same time, shuttle cabin housing produced 
      insignificant decrease in BAX gene expression in the hippocampus. In contrast to 
      the BCL-XL gene, genes encoding BAX, BDNF as well as TrkB and p75 receptors did 
      not respond to 30-day spaceflight. Thus, long-term spaceflight (1) did not affect 
      the expression of genes encoding BDNF as well as TrkB and p75 receptors, (2) 
      produced dysregulation in genetic control of the neuronal apoptosis, (3) 
      implicated BCL-XL as the risk factor for spaceflight-induced behavioral 
      abnormalities.
CI  - Copyright (c) 2014 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Naumenko, V S
AU  - Naumenko VS
AD  - Institute of Cytology and Genetics, Lavrentyeva Avenue, 10, 633090 Novosibirsk, 
      Russia. Electronic address: naumenko2002@bionet.nsc.ru.
FAU - Kulikov, A V
AU  - Kulikov AV
AD  - Institute of Cytology and Genetics, Lavrentyeva Avenue, 10, 633090 Novosibirsk, 
      Russia.
FAU - Kondaurova, E M
AU  - Kondaurova EM
AD  - Institute of Cytology and Genetics, Lavrentyeva Avenue, 10, 633090 Novosibirsk, 
      Russia.
FAU - Tsybko, A S
AU  - Tsybko AS
AD  - Institute of Cytology and Genetics, Lavrentyeva Avenue, 10, 633090 Novosibirsk, 
      Russia.
FAU - Kulikova, E A
AU  - Kulikova EA
AD  - Institute of Cytology and Genetics, Lavrentyeva Avenue, 10, 633090 Novosibirsk, 
      Russia.
FAU - Krasnov, I B
AU  - Krasnov IB
AD  - Institute of Biomedical Problems, Khoroshevskoe Street, 76a, 123007 Moscow, 
      Russia.
FAU - Shenkman, B S
AU  - Shenkman BS
AD  - Institute of Biomedical Problems, Khoroshevskoe Street, 76a, 123007 Moscow, 
      Russia.
FAU - Sychev, V N
AU  - Sychev VN
AD  - Institute of Biomedical Problems, Khoroshevskoe Street, 76a, 123007 Moscow, 
      Russia.
FAU - Bazhenova, E Y
AU  - Bazhenova EY
AD  - Institute of Cytology and Genetics, Lavrentyeva Avenue, 10, 633090 Novosibirsk, 
      Russia.
FAU - Sinyakova, N A
AU  - Sinyakova NA
AD  - Institute of Cytology and Genetics, Lavrentyeva Avenue, 10, 633090 Novosibirsk, 
      Russia.
FAU - Popova, N K
AU  - Popova NK
AD  - Institute of Cytology and Genetics, Lavrentyeva Avenue, 10, 633090 Novosibirsk, 
      Russia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141104
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Bax protein, mouse)
RN  - 0 (Bcl2l1 protein, mouse)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 0 (Ngfr protein, mouse)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 0 (bcl-X Protein)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain/*metabolism
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Gene Expression
MH  - Housing, Animal
MH  - Male
MH  - Mice, Inbred C57BL
MH  - RNA, Messenger/metabolism
MH  - Receptor, trkB/metabolism
MH  - Receptors, Nerve Growth Factor/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Space Flight
MH  - Stress, Physiological/physiology
MH  - bcl-2-Associated X Protein/metabolism
MH  - bcl-X Protein/metabolism
OTO - NOTNLM
OT  - BAX, BCL-X(L), BDNF, TrkB, p75 genes expression
OT  - environmental stress
OT  - mice
OT  - microgravity
OT  - spaceflight
EDAT- 2014/12/03 06:00
MHDA- 2015/12/31 06:00
CRDT- 2014/12/03 06:00
PHST- 2014/09/04 00:00 [received]
PHST- 2014/10/24 00:00 [revised]
PHST- 2014/10/26 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2015/12/31 06:00 [medline]
AID - S0306-4522(14)00917-8 [pii]
AID - 10.1016/j.neuroscience.2014.10.045 [doi]
PST - ppublish
SO  - Neuroscience. 2015 Jan 22;284:730-736. doi: 10.1016/j.neuroscience.2014.10.045. 
      Epub 2014 Nov 4.