PMID- 25451978
OWN - NLM
STAT- MEDLINE
DCOM- 20150812
LR  - 20181202
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Linking)
VI  - 400
DP  - 2015 Jan 15
TI  - In vitro differentiation between oxytocin- and vasopressin-secreting 
      magnocellular neurons requires more than one experimental criterion.
PG  - 102-11
LID - S0303-7207(14)00343-8 [pii]
LID - 10.1016/j.mce.2014.11.004 [doi]
AB  - The phenotypic differentiation between oxytocin (OT)- and vasopressin 
      (VP)-secreting magnocellular neurosecretory cells (MNCs) from the supraoptic 
      nucleus is relevant to understanding how several physiological and 
      pharmacological challenges affect their electrical activity. Although the firing 
      patterns of OT and VP neurons, both in vivo and in vitro, may appear different 
      from each other, much is assumed about their characteristics. These assumptions 
      make it practically impossible to obtain a confident phenotypic differentiation 
      based exclusively on the firing patterns. The presence of a sustained outward 
      rectifying potassium current (SOR) and/or an inward rectifying 
      hyperpolarization-activated current (IR), which are presumably present in OT 
      neurons and absent in VP neurons, has been used to distinguish between the two 
      types of MNCs in the past. In this study, we aimed to analyze the accuracy of the 
      phenotypic discrimination of MNCs based on the presence of rectifying currents 
      using comparisons with the molecular phenotype of the cells, as determined by 
      single-cell RT-qPCR and immunohistochemistry. Our results demonstrated that the 
      phenotypes classified according to the electrophysiological protocol in brain 
      slices do not match their molecular counterparts because vasopressinergic and 
      intermediate neurons also exhibit both outward and inward rectifying currents. In 
      addition, we also show that MNCs can change the relative proportion of each cell 
      phenotype when the system is challenged by chronic hypertonicity (70% water 
      restriction for 7 days). We conclude that for in vitro preparations, the 
      combination of mRNA detection and immunohistochemistry seems to be preferable 
      when trying to characterize a single MNC phenotype.
CI  - Copyright (c) 2014 Elsevier Ireland Ltd. All rights reserved.
FAU - da Silva, M P
AU  - da Silva MP
AD  - Department of Physiology, Ribeirao Preto Medical School, University of Sao Paulo, 
      Av. Bandeirantes, 3900, 14049-900 Ribeirao Preto, SP, Brazil.
FAU - Merino, R M
AU  - Merino RM
AD  - Department of Physiology, Ribeirao Preto Medical School, University of Sao Paulo, 
      Av. Bandeirantes, 3900, 14049-900 Ribeirao Preto, SP, Brazil.
FAU - Mecawi, A S
AU  - Mecawi AS
AD  - Department of Physiology, Faculty of Medicine, University of Malaysia, Malaysia.
FAU - Moraes, D J
AU  - Moraes DJ
AD  - Department of Physiology, Ribeirao Preto Medical School, University of Sao Paulo, 
      Av. Bandeirantes, 3900, 14049-900 Ribeirao Preto, SP, Brazil.
FAU - Varanda, W A
AU  - Varanda WA
AD  - Department of Physiology, Ribeirao Preto Medical School, University of Sao Paulo, 
      Av. Bandeirantes, 3900, 14049-900 Ribeirao Preto, SP, Brazil. Electronic address: 
      wvaranda@fmrp.usp.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141106
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (RNA, Messenger)
RN  - 0 (Sodium, Dietary)
RN  - 11000-17-2 (Vasopressins)
RN  - 50-56-6 (Oxytocin)
SB  - IM
MH  - Action Potentials/drug effects/*physiology
MH  - Animals
MH  - Diet
MH  - Gene Expression
MH  - Male
MH  - Microtomy
MH  - Neurons/classification/cytology/drug effects/*metabolism
MH  - Oxytocin/genetics/*metabolism
MH  - Patch-Clamp Techniques
MH  - Phenotype
MH  - RNA, Messenger/genetics/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Single-Cell Analysis
MH  - Sodium, Dietary/pharmacology
MH  - Supraoptic Nucleus/cytology/drug effects/*metabolism
MH  - Tissue Culture Techniques
MH  - Vasopressins/genetics/*metabolism
MH  - Water Deprivation
OTO - NOTNLM
OT  - Casopressin
OT  - Electrophysiology
OT  - Magnocellular neurons
OT  - Molecular identification
OT  - Rectifying currents
EDAT- 2014/12/03 06:00
MHDA- 2015/08/13 06:00
CRDT- 2014/12/03 06:00
PHST- 2014/08/01 00:00 [received]
PHST- 2014/11/04 00:00 [revised]
PHST- 2014/11/04 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2015/08/13 06:00 [medline]
AID - S0303-7207(14)00343-8 [pii]
AID - 10.1016/j.mce.2014.11.004 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2015 Jan 15;400:102-11. doi: 10.1016/j.mce.2014.11.004. Epub 
      2014 Nov 6.