PMID- 25454695
OWN - NLM
STAT- MEDLINE
DCOM- 20150706
LR  - 20220410
IS  - 1347-4421 (Electronic)
IS  - 1347-4421 (Linking)
VI  - 119
IP  - 4
DP  - 2015 Apr
TI  - Islets immunoisolation using encapsulation and PEGylation, simultaneously, as a 
      novel design.
PG  - 486-91
LID - S1389-1723(14)00359-4 [pii]
LID - 10.1016/j.jbiosc.2014.09.023 [doi]
AB  - The most important obstacle in islets transplantation for the treatment of 
      diabetes is graft rejection by the host immune system. To solve this problem, 
      immunosuppressive drugs should be used, but they may have several side effects. 
      To overcome these problems, islets immunoisolation systems such as encapsulation 
      and PEGylation have been developed. The aim of this study was to investigate the 
      possibility of using encapsulation and PEGylation techniques simultaneously (as a 
      novel design) for immunocamouflaging the islets of Langerhans. For this purpose, 
      the attachment of poly-L-ornithine (PLO) onto the surface of alginate 
      microcapsules and activated methoxy polyethylene glycol (mPEG) onto alginate-PLO 
      microcapsules was verified by Fourier transform infrared analysis and scanning 
      electron microscopy. Viability of the free and encapsulated islets up to the 7th 
      day was approved by acridine orange (AO)/propidium iodide (PI). The obtained 
      results from lymphocytes co-culturing with free and encapsulated islets (in 
      different designs of microcapsules with one to three layers) showed that 
      encapsulation generally reduces the immune response against the islets. However, 
      the addition of PLO and mPEG as second and third layers to the surface of 
      alginate microcapsules decreased interleukine-2 (IL-2) secretion against the 
      islets more and more. Finally, two different activated mPEG, mPEG-succinimidyl 
      carbonate (mPEG-SC) and mPEG-succinimidylvaleric acid (mPEG-SVA), used separately 
      on the surface of microcapsules were investigated, and the results showed that 
      IL-2 secretion was reduced 14.3% and 37.5% in comparison with the alginate-PLO 
      microcapsules, respectively. On the other hand, mPEG-SVA was more effective than 
      mPEG-SC, so it decreased IL-2 secretion 27.1% more than mPEG-SC.
CI  - Copyright (c) 2014 The Society for Biotechnology, Japan. Published by Elsevier B.V. 
      All rights reserved.
FAU - Nabavimanesh, Mohammad Mahdi
AU  - Nabavimanesh MM
AD  - Biotechnology Group, Faculty of Chemical Engineering, Tarbiat Modares University, 
      P. O. Box 14115-114, Tehran, Islamic Republic of Iran.
FAU - Hashemi-Najafabadi, Sameereh
AU  - Hashemi-Najafabadi S
AD  - Biotechnology Group, Faculty of Chemical Engineering, Tarbiat Modares University, 
      P. O. Box 14115-114, Tehran, Islamic Republic of Iran. Electronic address: 
      s.hashemi@modares.ac.ir.
FAU - Vasheghani-Farahani, Ebrahim
AU  - Vasheghani-Farahani E
AD  - Biotechnology Group, Faculty of Chemical Engineering, Tarbiat Modares University, 
      P. O. Box 14115-114, Tehran, Islamic Republic of Iran.
LA  - eng
PT  - Journal Article
DEP - 20141028
PL  - Japan
TA  - J Biosci Bioeng
JT  - Journal of bioscience and bioengineering
JID - 100888800
RN  - 0 (Alginates)
RN  - 0 (Capsules)
RN  - 0 (Hexuronic Acids)
RN  - 0 (Interleukin-2)
RN  - 0 (Peptides)
RN  - 0 (succinimidyl carbonate poly(ethylene glycol) monomethyl ether)
RN  - 25104-12-5 (polyornithine)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 8A5D83Q4RW (Glucuronic Acid)
RN  - 9004-74-4 (monomethoxypolyethylene glycol)
SB  - IM
MH  - Alginates/chemistry/metabolism
MH  - Animals
MH  - Capsules
MH  - Coculture Techniques
MH  - Glucuronic Acid/chemistry/metabolism
MH  - Hexuronic Acids/chemistry/metabolism
MH  - Interleukin-2/immunology/metabolism
MH  - Islets of Langerhans/cytology/*immunology/*metabolism
MH  - Islets of Langerhans Transplantation/*immunology/*methods
MH  - Lymphocytes/cytology/immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Electron, Scanning
MH  - Peptides/chemistry/metabolism
MH  - Polyethylene Glycols/chemistry/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Spectroscopy, Fourier Transform Infrared
OTO - NOTNLM
OT  - Encapsulation
OT  - Interleukine-2
OT  - Islets of Langerhans
OT  - Methoxy polyethylene glycol
OT  - PEGylation
OT  - Poly-l-ornithine
EDAT- 2014/12/03 06:00
MHDA- 2015/07/07 06:00
CRDT- 2014/12/03 06:00
PHST- 2014/05/12 00:00 [received]
PHST- 2014/09/23 00:00 [revised]
PHST- 2014/09/26 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2015/07/07 06:00 [medline]
AID - S1389-1723(14)00359-4 [pii]
AID - 10.1016/j.jbiosc.2014.09.023 [doi]
PST - ppublish
SO  - J Biosci Bioeng. 2015 Apr;119(4):486-91. doi: 10.1016/j.jbiosc.2014.09.023. Epub 
      2014 Oct 28.