PMID- 25454862
OWN - NLM
STAT- MEDLINE
DCOM- 20150723
LR  - 20141203
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Linking)
VI  - 32
IP  - 50
DP  - 2014 Nov 28
TI  - Enhanced immune response of BMDCs pulsed with H9N2 AIV and CpG.
PG  - 6783-90
LID - S0264-410X(14)01374-7 [pii]
LID - 10.1016/j.vaccine.2014.10.013 [doi]
AB  - Dendritic cells (DCs), professional antigen presenting cells, have demonstrated 
      effective in controlling the initial of innate immune, while CpG could improve 
      the performance of immune system. To explore the mechanism of CpG enhancing the 
      immune response, we compared different stimulated mouse DCs with systemic 
      approach microarrays. Analysis revealed 1840 differentially expressed genes in 
      H9N2 stimulated group, more than 1728 altered genes in inactive H9N2 group. 
      Investigation also proved that CpG/inactive H9N2 co-stimulation changed 2140 
      genes, more than that in H9N2 group, strongly demonstrated that CpG improved the 
      performance of inactive H9N2 vaccination. Pathways analysis founded that DCs 
      response rapid to shift in their maturation state, which involved Toll-like 
      receptor (TLR) pathway significantly. Microarrays results were also verified by 
      qRT-PCR with 14 elected representative genes. Further analysis proved that 
      co-stimulatory molecules (CD40, CD80, CD86 and MHC-II), regulatory protein (IRF-7 
      and TRAF-6) and pro-inflammatory cytokines (IL-1, IL-6 and IL-12) were all 
      changed and involved in DCs maturation. At last we demonstrated TLR signalling 
      pathway in chicken bone marrow-derived dendritic cells (chBM-DCs) stimulated with 
      CpG. The distinct transcriptional profiles of DCs pulsed with various stimuli 
      expanded our understanding of how DCs respond and recognize influenza.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Lin, Jian
AU  - Lin J
AD  - Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing 
      Agricultural University, China; Nanjing Agricultural University, Wei gang 1, 
      Jiangsu, PR China.
FAU - Yin, Yin Y
AU  - Yin YY
AD  - Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing 
      Agricultural University, China; Nanjing Agricultural University, Wei gang 1, 
      Jiangsu, PR China.
FAU - Qin, Tao
AU  - Qin T
AD  - Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing 
      Agricultural University, China; Nanjing Agricultural University, Wei gang 1, 
      Jiangsu, PR China.
FAU - Zhu, Li Q
AU  - Zhu LQ
AD  - Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing 
      Agricultural University, China; Nanjing Agricultural University, Wei gang 1, 
      Jiangsu, PR China.
FAU - Yu, Qing H
AU  - Yu QH
AD  - Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing 
      Agricultural University, China; Nanjing Agricultural University, Wei gang 1, 
      Jiangsu, PR China.
FAU - Yang, Qian
AU  - Yang Q
AD  - Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing 
      Agricultural University, China. Electronic address: zxbyq@njau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141022
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (CPG-oligonucleotide)
RN  - 0 (Influenza Vaccines)
RN  - 0 (Oligodeoxyribonucleotides)
SB  - IM
MH  - Adjuvants, Immunologic/*metabolism
MH  - Animals
MH  - Dendritic Cells/*immunology/*virology
MH  - Gene Expression Profiling
MH  - Influenza A Virus, H9N2 Subtype/*immunology
MH  - Influenza Vaccines/*immunology
MH  - Mice, Inbred C57BL
MH  - Microarray Analysis
MH  - Oligodeoxyribonucleotides/*metabolism
MH  - Real-Time Polymerase Chain Reaction
OTO - NOTNLM
OT  - Active or inactive H9N2
OT  - CpG
OT  - Dendritic cells
OT  - Immune responses
OT  - TLR signal pathway
EDAT- 2014/12/03 06:00
MHDA- 2015/07/24 06:00
CRDT- 2014/12/03 06:00
PHST- 2014/06/19 00:00 [received]
PHST- 2014/09/02 00:00 [revised]
PHST- 2014/10/03 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2015/07/24 06:00 [medline]
AID - S0264-410X(14)01374-7 [pii]
AID - 10.1016/j.vaccine.2014.10.013 [doi]
PST - ppublish
SO  - Vaccine. 2014 Nov 28;32(50):6783-90. doi: 10.1016/j.vaccine.2014.10.013. Epub 
      2014 Oct 22.