PMID- 25455740
OWN - NLM
STAT- MEDLINE
DCOM- 20160321
LR  - 20220409
IS  - 1477-2566 (Electronic)
IS  - 1465-3249 (Linking)
VI  - 17
IP  - 2
DP  - 2015 Feb
TI  - Influence of inflammation on the immunological profile of adult-derived human 
      liver mesenchymal stromal cells and stellate cells.
PG  - 174-85
LID - S1465-3249(14)00801-9 [pii]
LID - 10.1016/j.jcyt.2014.10.001 [doi]
AB  - BACKGROUND AIMS: Stem cell therapy for liver diseases has recently emerged as a 
      promising alternative to liver transplantation. Eligible cells should have an 
      appropriate immunophenotype. The aim of the present study was to define the 
      immunological profile of two human liver-derived mesenchymal stromal cell 
      populations, namely, stem cells (ADHLSC) and hepatic stellate cells (HSC). 
      METHODS: The study was conducted under normal and inflammatory conditions with 
      the use of human bone marrow mesenchymal stromal cells (BM-MSC) as reference. 
      RESULTS: Like BM-MSC and ADHLSC, HSC were negative for hematopoietic (CD45) and 
      endothelial (CD34) markers but positive for stromal markers. All cell types were 
      constitutively positive for HLA class I and negative for human leukocyte antigen 
      (HLA) class II and co-stimulatory molecules (CD80, CD86, CD134 and CD252). 
      Inflammation induced the expression of CD40 in all cell types, but the highest 
      values were observed on HSCs; high CD252 expression was only observed on HSC as 
      compared with ADHLSC and BM-MSC. The expression of various adhesion molecules 
      (CD54, CD58, CD106 and CD166) was dissimilar in these three cell types and was 
      differentially influenced by inflammation as well. ADHLSC and HSC constitutively 
      expressed the immunosuppressive molecule HLA-G, whereas CD274 expression was 
      induced by inflammation, as in the case of BM-MSC. Moreover, all cell types 
      expressed the two major natural killer ligands CD112 and CD115. CONCLUSIONS: 
      Toll-like receptors (TLR) 1, 3, 4 and 6 messenger RNA was expressed by both cell 
      types, whereas TLR 2, 5, 7, 9 and 10 were only expressed by ADHLSC. Inflammation 
      increased the expression of TLR 2 and 3 by ADHLSC and HSC. Finally, both 
      liver-derived cell types were immunosuppressive because they inhibited the 
      proliferation of mitogen-activated T cells.
CI  - Copyright (c) 2015 International Society for Cellular Therapy. Published by 
      Elsevier Inc. All rights reserved.
FAU - Raicevic, Gordana
AU  - Raicevic G
AD  - Laboratory of Clinical Cell Therapy, Institut Jules Bordet, Universite Libre de 
      Bruxelles, Brussels, Belgium. Electronic address: gordana.raicevic@ulb.ac.be.
FAU - Najar, Mehdi
AU  - Najar M
AD  - Laboratory of Clinical Cell Therapy, Institut Jules Bordet, Universite Libre de 
      Bruxelles, Brussels, Belgium.
FAU - Najimi, Mustapha
AU  - Najimi M
AD  - Laboratory of Pediatric Hepatology and Cell Therapy, Institute of Experimental & 
      Clinical Research, Universite Catholique de Louvain, Brussels, Belgium.
FAU - El Taghdouini, Adil
AU  - El Taghdouini A
AD  - Liver Cell Biology Laboratory, Vrije Universiteit Brussel, Brussels, Belgium.
FAU - van Grunsven, Leo A
AU  - van Grunsven LA
AD  - Liver Cell Biology Laboratory, Vrije Universiteit Brussel, Brussels, Belgium.
FAU - Sokal, Etienne
AU  - Sokal E
AD  - Laboratory of Pediatric Hepatology and Cell Therapy, Institute of Experimental & 
      Clinical Research, Universite Catholique de Louvain, Brussels, Belgium.
FAU - Toungouz, Michel
AU  - Toungouz M
AD  - Laboratory of Clinical Cell Therapy, Institut Jules Bordet, Universite Libre de 
      Bruxelles, Brussels, Belgium; Hopital Erasme, Universite Libre de Bruxelles, 
      Brussels, Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141121
PL  - England
TA  - Cytotherapy
JT  - Cytotherapy
JID - 100895309
RN  - 0 (Antigens, CD34)
RN  - 0 (B7-H1 Antigen)
RN  - 0 (Biomarkers)
RN  - 0 (HLA-G Antigens)
RN  - 0 (IL2RB protein, human)
RN  - 0 (Interleukin-2 Receptor beta Subunit)
RN  - 0 (Toll-Like Receptors)
RN  - EC 2.7.10.1 (Receptor, Macrophage Colony-Stimulating Factor)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Antigens, CD34/metabolism
MH  - B7-H1 Antigen/metabolism
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/cytology
MH  - Cell- and Tissue-Based Therapy
MH  - HLA-G Antigens/metabolism
MH  - Hematopoietic Stem Cells/immunology
MH  - Hepatic Stellate Cells/cytology/*immunology
MH  - Humans
MH  - Immunomodulation/*immunology
MH  - Immunophenotyping
MH  - Inflammation/*immunology
MH  - Interleukin-2 Receptor beta Subunit/metabolism
MH  - Leukocyte Common Antigens/metabolism
MH  - Liver/cytology
MH  - Liver Diseases/therapy
MH  - Lymphocyte Activation/*immunology
MH  - Mesenchymal Stem Cells/cytology/*immunology
MH  - Receptor, Macrophage Colony-Stimulating Factor/metabolism
MH  - Toll-Like Receptors/biosynthesis
OTO - NOTNLM
OT  - Toll-like receptors
OT  - adult-derived human liver stem cells
OT  - cell adhesion molecules
OT  - inflammation
OT  - stellate cells
EDAT- 2014/12/03 06:00
MHDA- 2016/03/22 06:00
CRDT- 2014/12/03 06:00
PHST- 2014/04/25 00:00 [received]
PHST- 2014/10/02 00:00 [revised]
PHST- 2014/10/03 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2016/03/22 06:00 [medline]
AID - S1465-3249(14)00801-9 [pii]
AID - 10.1016/j.jcyt.2014.10.001 [doi]
PST - ppublish
SO  - Cytotherapy. 2015 Feb;17(2):174-85. doi: 10.1016/j.jcyt.2014.10.001. Epub 2014 
      Nov 21.