PMID- 25457367
OWN - NLM
STAT- MEDLINE
DCOM- 20160502
LR  - 20191210
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 174
IP  - 3-4
DP  - 2014 Dec 5
TI  - GP5 expression in Marc-145 cells inhibits porcine reproductive and respiratory 
      syndrome virus infection by inducing beta interferon activity.
PG  - 409-418
LID - S0378-1135(14)00461-1 [pii]
LID - 10.1016/j.vetmic.2014.09.030 [doi]
AB  - The major neutralizing epitope of porcine reproductive and respiratory syndrome 
      virus (PRRSV) is mainly located on virus glycoprotein 5 (GP5). Immunization with 
      exogenous GP5 or exposure to native GP5 by means of DNA immunization can provide 
      some degree of immune protection to PRRSV infection in pigs. However, during 
      PRRSV infection in pigs, the production of neutralization antibodies induced by 
      GP5 is delayed or suppressed. This suggests that the synthesis of GP5 is late 
      than some PRRSV proteins or other PRRSV proteins interfering with the function of 
      GP5 in inducing host responses during virus infection. Here, to exclude the 
      impacts of the other PRRSV proteins and determine the role of GP5 in the 
      replication of PRRSV in vitro, a Marc-145 cell line stably expressing GP5 
      (Marc-145-GP5(Flag)) was constructed. Cell proliferation and cell apoptosis 
      measurements indicated that the expression of GP5 in Marc-145 cells did not 
      disturb the cells' viability. Following infection with different PRRSV strains 
      PRRSV replication in Marc-145-GP5(Flag) cells was inhibited significantly. Type I 
      interferon assay results showed that beta interferon (IFN-beta) in the 
      Marc-145-GP5(Flag) cells were increased at mRNA and protein levels. When siRNA 
      was introduced into the cells to knock down IFN-beta mRNA, PRRSV infectivity of 
      these cells was recovered. These data suggest that early GP5 expression is not 
      favorable for further infection by PRRSV, because it not only stimulates 
      production of neutralization antibodies in pigs, but also induces IFN-beta 
      production in host cells. Therefore, GP5 is an important protein in the induction 
      of self-protection responses from the host.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Gao, Jiming
AU  - Gao J
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling 712100, China; Experimental Station of 
      Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of 
      the People's Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, 
      China. Electronic address: jimingao2006@163.com.
FAU - Ji, Pengchao
AU  - Ji P
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling 712100, China; Experimental Station of 
      Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of 
      the People's Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, 
      China. Electronic address: tiankonglan246@163.com.
FAU - Zhang, Maodong
AU  - Zhang M
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling 712100, China; Experimental Station of 
      Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of 
      the People's Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, 
      China. Electronic address: maozhangdong@163.com.
FAU - Wang, Xiangpeng
AU  - Wang X
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling 712100, China; Experimental Station of 
      Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of 
      the People's Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, 
      China. Electronic address: wangxiangpeng2003@126.com.
FAU - Li, Na
AU  - Li N
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling 712100, China; Experimental Station of 
      Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of 
      the People's Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, 
      China.
FAU - Wang, Chengbao
AU  - Wang C
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling 712100, China; Experimental Station of 
      Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of 
      the People's Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, 
      China. Electronic address: wangchengbao@126.com.
FAU - Xiao, Shuqi
AU  - Xiao S
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling 712100, China; Experimental Station of 
      Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of 
      the People's Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, 
      China. Electronic address: shqxiaojd@hotmail.com.
FAU - Mu, Yang
AU  - Mu Y
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling 712100, China; Experimental Station of 
      Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of 
      the People's Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, 
      China. Electronic address: muyang@nwsuaf.edu.cn.
FAU - Zhao, Qin
AU  - Zhao Q
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling 712100, China; Experimental Station of 
      Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of 
      the People's Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, 
      China. Electronic address: qinzhao_2004@sina.com.
FAU - Du, Taofeng
AU  - Du T
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling 712100, China; Experimental Station of 
      Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of 
      the People's Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, 
      China. Electronic address: taofengdu@126.com.
FAU - Sun, Yani
AU  - Sun Y
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling 712100, China; Experimental Station of 
      Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of 
      the People's Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, 
      China. Electronic address: yanisun_2005@sina.com.
FAU - Hiscox, Julian A
AU  - Hiscox JA
AD  - Department of Infection Biology, Institute of Infection and Global Health, 
      University of Liverpool, Liverpool L3 5RF, UK. Electronic address: 
      julian.hiscox@liverpool.ac.uk.
FAU - Zhang, Gaiping
AU  - Zhang G
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling 712100, China; College of Animal Science and 
      Veterinary Medicine, Henan Agriculture University, Zhengzhou 450002, China. 
      Electronic address: zhanggaiping2003@yahoo.com.cn.
FAU - Zhou, En-Min
AU  - Zhou EM
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling 712100, China; Experimental Station of 
      Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of 
      the People's Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100, 
      China. Electronic address: zhouem@nwsuaf.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141014
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Epitopes)
RN  - 0 (Glycoproteins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
RN  - 77238-31-4 (Interferon-beta)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Cell Line
MH  - Cell Survival
MH  - Chlorocebus aethiops
MH  - Epitopes
MH  - Glycoproteins
MH  - Interferon-beta/*metabolism
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - RNA, Small Interfering
MH  - Swine
MH  - Viral Envelope Proteins/*genetics
OTO - NOTNLM
OT  - Cell apoptosis
OT  - Cell viability
OT  - GP5
OT  - Interferon
OT  - PRRSV
EDAT- 2014/12/03 06:00
MHDA- 2016/05/03 06:00
CRDT- 2014/12/03 06:00
PHST- 2014/05/13 00:00 [received]
PHST- 2014/09/24 00:00 [revised]
PHST- 2014/09/25 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2016/05/03 06:00 [medline]
AID - S0378-1135(14)00461-1 [pii]
AID - 10.1016/j.vetmic.2014.09.030 [doi]
PST - ppublish
SO  - Vet Microbiol. 2014 Dec 5;174(3-4):409-418. doi: 10.1016/j.vetmic.2014.09.030. 
      Epub 2014 Oct 14.