PMID- 2545892
OWN - NLM
STAT- MEDLINE
DCOM- 19890822
LR  - 20190510
IS  - 0027-8874 (Print)
IS  - 0027-8874 (Linking)
VI  - 81
IP  - 15
DP  - 1989 Aug 2
TI  - mRNA expression of transforming growth factor alpha in human breast carcinomas 
      and its activity in effusions of breast cancer patients.
PG  - 1165-71
AB  - Transforming growth factor alpha (TGF alpha) mRNA expression was measured by 
      Northern blot analysis in 18 human, primary, infiltrating, ductal breast 
      carcinomas. Expression of a 4.8-kilobase TGF alpha mRNA transcript was detected 
      in nine of 18 tumors. No evidence was observed of any gross amplifications or 
      major rearrangements of the TGF alpha gene in the breast carcinoma specimens. 
      Biologically active and immunoreactive TGF alpha was measured in the pleural 
      effusions or in the ascitic fluids from 37 noncancer and 63 cancer patients. The 
      TGF alpha activity detected ranged from 0.2 to 26 ng/mL in most effusions from 
      both groups. However, 29 of 63 (46%) of the effusions from cancer patients 
      exhibited TGF alpha levels that were 6 ng/mL or higher, whereas only seven of 37 
      (19%) of those from noncancer patients exceeded this level (P less than .03). In 
      particular, effusions obtained from breast cancer patients showed a significantly 
      higher level of TGF alpha, compared with those from noncancer patients (P less 
      than .001). Effusions from 14 cancer patients also contained elevated levels of 
      two tumor-associated antigens, CEA and/or TAG-72, and within this group, nine 
      also had elevated levels of TGF alpha.
FAU - Ciardiello, F
AU  - Ciardiello F
AD  - Laboratory of Tumor Immunology and Biology, National Cancer Institute, Bethesda, 
      MD 20892.
FAU - Kim, N
AU  - Kim N
FAU - Liscia, D S
AU  - Liscia DS
FAU - Bianco, C
AU  - Bianco C
FAU - Lidereau, R
AU  - Lidereau R
FAU - Merlo, G
AU  - Merlo G
FAU - Callahan, R
AU  - Callahan R
FAU - Greiner, J
AU  - Greiner J
FAU - Szpak, C
AU  - Szpak C
FAU - Kidwell, W
AU  - Kidwell W
AU  - et al.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Natl Cancer Inst
JT  - Journal of the National Cancer Institute
JID - 7503089
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 76057-06-2 (Transforming Growth Factors)
SB  - IM
MH  - Ascitic Fluid/metabolism
MH  - Blotting, Northern
MH  - Breast Neoplasms/*metabolism
MH  - Carcinoma, Intraductal, Noninfiltrating/*metabolism/secondary
MH  - Female
MH  - Humans
MH  - Lymphatic Metastasis
MH  - Pleural Effusion/metabolism
MH  - RNA, Messenger/*analysis
MH  - RNA, Neoplasm/*analysis
MH  - Transforming Growth Factors/*biosynthesis/genetics
EDAT- 1989/08/02 00:00
MHDA- 1989/08/02 00:01
CRDT- 1989/08/02 00:00
PHST- 1989/08/02 00:00 [pubmed]
PHST- 1989/08/02 00:01 [medline]
PHST- 1989/08/02 00:00 [entrez]
AID - 10.1093/jnci/81.15.1165 [doi]
PST - ppublish
SO  - J Natl Cancer Inst. 1989 Aug 2;81(15):1165-71. doi: 10.1093/jnci/81.15.1165.