PMID- 25460834
OWN - NLM
STAT- MEDLINE
DCOM- 20150727
LR  - 20181113
IS  - 1872-7298 (Electronic)
IS  - 1567-133X (Print)
IS  - 1567-133X (Linking)
VI  - 16
IP  - 2
DP  - 2014 Nov
TI  - Temporal and spatial expression of tight junction genes during zebrafish 
      pronephros development.
PG  - 104-13
LID - S1567-133X(14)00091-X [pii]
LID - 10.1016/j.gep.2014.11.001 [doi]
AB  - The kidney is comprised of nephrons - epithelial tubes with specialized segments 
      that reabsorb and secrete solutes, perform osmoregulation, and produce urine. 
      Different nephron segments exhibit unique combinations of ion channels, 
      transporter proteins, and cell junction proteins that govern permeability between 
      neighboring cells. The zebrafish pronephros is a valuable model to study the 
      mechanisms of vertebrate nephrogenesis, but many basic features of segment gene 
      expression in renal progenitors and mature nephrons have not been characterized. 
      Here, we analyzed the temporal and spatial expression pattern of tight junction 
      components during zebrafish kidney ontogeny. During nephrogenesis, renal 
      progenitors show discrete expression domains of claudin (cldn) 15a, cldn8, 
      occludin (ocln) a, oclnb, tight junction protein (tjp) 2a, tjp2b, and tjp3. 
      Interestingly, transcripts encoding these genes exhibit dynamic spatiotemporal 
      domains during the time when pronephros segment domains are established. These 
      data provide a useful gene expression map of cell junction components during 
      zebrafish nephrogenesis. As such, this information complements the existing 
      molecular map of nephron segment characteristics, and can be used to characterize 
      kidney development mutants as well as various disease models, in addition to 
      aiding in the elucidation of mechanisms governing epithelial regeneration after 
      acute nephron injury.
CI  - Copyright (c) 2014 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - McKee, Robert
AU  - McKee R
AD  - Department of Biological Sciences and Center for Zebrafish Research, University 
      of Notre Dame, Notre Dame, IN 46556, USA.
FAU - Gerlach, Gary F
AU  - Gerlach GF
AD  - Department of Biological Sciences and Center for Zebrafish Research, University 
      of Notre Dame, Notre Dame, IN 46556, USA.
FAU - Jou, Jonathan
AU  - Jou J
AD  - Department of Biological Sciences and Center for Zebrafish Research, University 
      of Notre Dame, Notre Dame, IN 46556, USA.
FAU - Cheng, Christina N
AU  - Cheng CN
AD  - Department of Biological Sciences and Center for Zebrafish Research, University 
      of Notre Dame, Notre Dame, IN 46556, USA.
FAU - Wingert, Rebecca A
AU  - Wingert RA
AD  - Department of Biological Sciences and Center for Zebrafish Research, University 
      of Notre Dame, Notre Dame, IN 46556, USA. Electronic address: rwingert@nd.edu.
LA  - eng
GR  - DP2 OD008470/OD/NIH HHS/United States
GR  - K01 DK083512/DK/NIDDK NIH HHS/United States
GR  - R01 DK100237/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20141107
PL  - Netherlands
TA  - Gene Expr Patterns
JT  - Gene expression patterns : GEP
JID - 101167473
RN  - 0 (Zebrafish Proteins)
SB  - IM
MH  - Animals
MH  - *Gene Expression Regulation, Developmental
MH  - In Situ Hybridization
MH  - Nephrons/embryology/metabolism
MH  - Organogenesis/genetics
MH  - Pronephros/*embryology
MH  - Tight Junctions/*genetics/metabolism
MH  - Transcription, Genetic
MH  - Zebrafish/*embryology/*genetics
MH  - Zebrafish Proteins/*genetics/metabolism
PMC - PMC4262568
MID - NIHMS641388
OTO - NOTNLM
OT  - Claudin
OT  - Kidney
OT  - Occludin
OT  - Renal progenitor
OT  - Tight junction protein
OT  - Zebrafish
EDAT- 2014/12/03 06:00
MHDA- 2015/07/28 06:00
PMCR- 2015/11/07
CRDT- 2014/12/03 06:00
PHST- 2014/07/24 00:00 [received]
PHST- 2014/11/03 00:00 [revised]
PHST- 2014/11/04 00:00 [accepted]
PHST- 2014/12/03 06:00 [entrez]
PHST- 2014/12/03 06:00 [pubmed]
PHST- 2015/07/28 06:00 [medline]
PHST- 2015/11/07 00:00 [pmc-release]
AID - S1567-133X(14)00091-X [pii]
AID - 10.1016/j.gep.2014.11.001 [doi]
PST - ppublish
SO  - Gene Expr Patterns. 2014 Nov;16(2):104-13. doi: 10.1016/j.gep.2014.11.001. Epub 
      2014 Nov 7.